PIGA (NM_020473) Human Untagged Clone
CAT#: SC317265
PIGA (untagged)-Human phosphatidylinositol glycan anchor biosynthesis, class A (PIGA), transcript variant 3
"NM_020473" in other vectors (5)
Product Images
Specifications
Product Data | |
Type | Human Untagged Clone |
Tag | Tag Free |
Symbol | PIGA |
Synonyms | GPI3; MCAHS2; PIG-A; PNH1 |
Vector | pCMV6 series |
Sequence Data |
>NCBI ORF sequence for NM_020473, the custom clone sequence may differ by one or more nucleotides
ATGGCGATCGTGGAAGCAGCCAGTTGTGGTTTACAGGTTGTAAGTACCAGAGTTGGTGGA ATTCCTGAGGTGCTTCCAGAAAACCTTATTATTTTATGTGAGCCTTCAGTAAAATCTTTG TGTGAAGGATTGGAAAAGGCTATTTTCCAACTGAAGTCAGGGACATTGCCAGCTCCAGAA AACATCCATAACATAGTAAAGACTTTCTACACCTGGAGGAATGTTGCAGAAAGAACTGAA AAGGTATATGACCGGGTATCAGTGGAAGCTGTGTTGCCAATGGACAAACGACTGGACAGA CTTATTTCTCACTGCGGCCCAGTAACAGGCTACATCTTTGCTTTGTTGGCAGTTTTCAAC TTCCTCTTCCTCATTTTCTTGAGATGGATGACTCCAGATTCTATCATTGATGTTGCAATA GATGCCACTGGGCCACGGGGTGCCTGGACTAATAACTATTCTCACAGTAAAAGAGGGGGT GAGAATAATGAGATATCTGAAACCAGG |
Restriction Sites | Please inquire |
ACCN | NM_020473 |
Insert Size | 2867 bp |
OTI Disclaimer | Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP). |
OTI Annotation | This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA. |
Product Components | The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_020473.2, NP_065206.2 |
RefSeq Size | 2867 bp |
RefSeq ORF | 510 bp |
Locus ID | 5277 |
Cytogenetics | Xp22.2 |
Protein Families | Transmembrane |
Protein Pathways | Glycosylphosphatidylinositol(GPI)-anchor biosynthesis, Metabolic pathways |
Gene Summary | 'This gene encodes a protein required for synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the biosynthetic pathway of GPI anchor. The GPI anchor is a glycolipid found on many blood cells and which serves to anchor proteins to the cell surface. Paroxysmal nocturnal hemoglobinuria, an acquired hematologic disorder, has been shown to result from mutations in this gene. Alternate splice variants have been characterized. A related pseudogene is located on chromosome 12. [provided by RefSeq, Jun 2010]' Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region and uses an alternate start codon, compared to variant 1. The resulting isoform (3) has a distinct and shorter N-terminus, compared to isoform 1. |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC109568 | PIGA (untagged)-Human phosphatidylinositol glycan anchor biosynthesis, class A (PIGA), transcript variant 3 |
USD 420.00 |
|
RC207733 | PIGA (Myc-DDK-tagged)-Human phosphatidylinositol glycan anchor biosynthesis, class A (PIGA), transcript variant 3 |
USD 420.00 |
|
RG207733 | PIGA (GFP-tagged) - Human phosphatidylinositol glycan anchor biosynthesis, class A (PIGA), transcript variant 3 |
USD 460.00 |
|
RC207733L3 | Lenti-ORF clone of PIGA (Myc-DDK-tagged)-Human phosphatidylinositol glycan anchor biosynthesis, class A (PIGA), transcript variant 3 |
USD 620.00 |
|
RC207733L4 | Lenti-ORF clone of PIGA (mGFP-tagged)-Human phosphatidylinositol glycan anchor biosynthesis, class A (PIGA), transcript variant 3 |
USD 620.00 |
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