KCNK2 (NM_001017425) Human Untagged Clone

CAT#: SC317688

KCNK2 (untagged)-Human potassium channel, subfamily K, member 2 (KCNK2), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KCNK2
• Synonyms: hTREK-1c; hTREK-1e; K2p2.1; TPKC1; TREK; TREK-1; TREK1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001017425, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCTTCCCAGCGCCTCGCGGGAGAGACCCGGCTATAGAGCAGGAGTGGCGGCACCTGACTTGCTGGATC
  CTAAATCTGCCGCTCAGAACTCCAAACCGAGGCTCTCGTTTTCCACGAAACCCACAGTGCTTGCTTCCCG
  GGTGGAGAGTGACACGACCATTAATGTTATGAAATGGAAGACGGTCTCCACGATATTCCTGGTGGTTGTC
  CTCTATCTGATCATCGGAGCCACCGTGTTCAAAGCATTGGAGCAGCCTCATGAGATTTCACAGAGGACCA
  CCATTGTGATCCAGAAGCAAACATTCATATCCCAACATTCCTGTGTCAATTCGACGGAGCTGGATGAACT
  CATTCAGCAAATAGTGGCAGCAATAAATGCAGGGATTATACCGTTAGGAAACACCTCCAATCAAATCAGT
  CACTGGGATTTGGGAAGTTCCTTCTTCTTTGCTGGCACTGTTATTACAACCATAGGATTTGGAAACATCT
  CACCACGCACAGAAGGCGGCAAAATATTCTGTATCATCTATGCCTTACTGGGAATTCCCCTCTTTGGTTT
  TCTCTTGGCTGGAGTTGGAGATCAGCTAGGCACCATATTTGGAAAAGGAATTGCCAAAGTGGAAGATACG
  TTTATTAAGTGGAATGTTAGTCAGACCAAGATTCGCATCATCTCAACAATCATATTTATACTATTTGGCT
  GTGTACTCTTTGTGGCTCTGCCTGCGATCATATTCAAACACATAGAAGGCTGGAGTGCCCTGGACGCCAT
  TTATTTTGTGGTTATCACTCTAACAACTATTGGATTTGGTGACTACGTTGCAGGTGGATCCGATATTGAA
  TATCTGGACTTCTATAAGCCTGTCGTGTGGTTCTGGATCCTTGTAGGGCTTGCTTACTTTGCTGCTGTCC
  TGAGCATGATTGGAGATTGGCTCCGAGTGATATCTAAAAAGACAAAAGAAGAGGTGGGAGAGTTCAGAGC
  ACACGCTGCTGAGTGGACAGCCAACGTCACAGCCGAATTCAAAGAAACCAGGAGGCGACTGAGTGTGGAG
  ATTTATGACAAGTTCCAGCGGGCCACCTCCATCAAGCGGAAGCTCTCGGCAGAACTGGCTGGAAACCACA
  ATCAGGAGCTGACTCCTTGTAGGAGGACCCTGTCAGTGAACCACCTGACCAGCGAGAGGGATGTCTTGCC
  TCCCTTACTGAAGACTGAGAGTATCTATCTGAATGGTTTGACGCCACACTGTGCTGGTGAAGAGATTGCT
  GTGATTGAGAACATCAAATAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001017425
• Insert Size: 1281 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001017425.2, NP_001017425.2
• RefSeq Size: 3398 bp
• RefSeq ORF: 1281 bp
• Locus ID: 3776
• Cytogenetics: 1q41
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• Gene Summary: 'This gene encodes one of the members of the two-pore-domain background potassium channel protein family. This type of potassium channel is formed by two homodimers that create a channel that leaks potassium out of the cell to control resting membrane potential. The channel can be opened, however, by certain anesthetics, membrane stretching, intracellular acidosis, and heat. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (3) differs in the 5' UTR and beginning of the coding region compared to variant 1. The resulting isoform (c) is slightly shorter and has a distinct N-terminus compared to isoform a.