Esa1 (FLOT2) (NM_004475) Human Untagged Clone

CAT#: SC317689

FLOT2 (untagged)-Human flotillin 2 (FLOT2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FLOT2
• Synonyms: ECS-1; ECS1; ESA; ESA1; M17S1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004475, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGCAATTGCCACACGGTGGGGCCCAACGAGGCGCTGGTGGTTTCAGGGGGCTGTTGTGGTTCCGACT
  ATAAACAGTACGTGTTTGGCGGCTGGGCCTGGGCCTGGTGGTGTATCTCCGACACTCAGAGGATTTCCCT
  AGAGATTATGACGTTGCAGCCCCGCTGCGAGGACGTAGAGACGGCCGAGGGGGTAGCTTTAACTGTGACG
  GGTGTCGCCCAGGTGAAGATCATGACGGAGAAGGAACTCCTGGCCGTGGCTTGTGAGCAGTTTCTGGGTA
  AGAATGTGCAGGACATCAAAAACGTCGTCCTGCAGACCCTGGAGGGACATCTGCGCTCCATCCTCGGGAC
  CCTGACAGTGGAGCAGATTTATCAGGACCGGGACCAGTTTGCCAAGCTGGTGCGGGAGGTGGCAGCCCCT
  GATGTTGGCCGCATGGGCATTGAGATCCTCAGCTTCACCATCAAGGACGTGTATGACAAAGTGGACTATC
  TGAGCTCCCTGGGCAAGACGCAGACTGCCGTGGTGCAGAGAGATGCTGACATTGGCGTGGCCGAGGCTGA
  ACGGGACGCAGGCATCCGGGAAGCTGAGTGCAAGAAGGAGATGCTGGATGTGAAGTTCATGGCAGACACC
  AAGATTGCTGACTCTAAGCGAGCCTTCGAGCTGCAAAAGTCAGCCTTCAGTGAGGAGGTTAACATCAAGA
  CAGCTGAGGCCCAGTTGGCCTATGAGCTGCAGGGGGCCCGTGAACAGCAGAAGATCCGGCAGGAAGAGAT
  TGAGATTGAGGTTGTGCAGCGCAAGAAACAGATTGCCGTGGAGGCACAGGAGATCCTGCGTACGGACAAG
  GAGCTCATCGCTACAGTGCGCCGGCCTGCCGAGGCCGAGGCCCACCGCATCCAGCAGATTGCCGAGGGTG
  AAAAGGTGAAGCAGGTCCTCTTGGCACAGGCAGAGGCTGAGAAGATCCGCAAAATCGGGGAGGCGGAAGC
  GGCAGTCATCGAGGCGATGGGCAAGGCAGAGGCTGAGCGGATGAAGCTCAAGGCAGAAGCCTACCAGAAA
  TACGGGGATGCAGCCAAGATGGCCTTGGTGCTAGAGGCCCTGCCCCAGATTGCTGCCAAAATCGCTGCCC
  CACTTACCAAGGTCGATGAGATTGTGGTCCTCAGTGGAGACAACAGTAAGGTCACATCAGAAGTGAACCG
  ACTGCTGGCCGAGCTGCCTGCCTCTGTGCATGCCCTCACAGGCGTGGACCTGTCTAAGATACCCCTGATC
  AAGAAGGCCACTGGTGTGCAGGTGTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_004475
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004475.2, NP_004466.2
• RefSeq Size: 2659 bp
• RefSeq ORF: 1287 bp
• Locus ID: 2319
• Cytogenetics: 17q11.2
• Domains: Band_7, Flotillin
• Protein Families: Druggable Genome
• Protein Pathways: Insulin signaling pathway
• Gene Summary: 'Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. This gene encodes a caveolae-associated, integral membrane protein, which is thought to function in neuronal signaling. [provided by RefSeq, Jul 2008]'