KRT35 (NM_002280) Human Untagged Clone

CAT#: SC317748

KRT35 (untagged)-Human keratin 35 (KRT35)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KRT35
• Synonyms: Ha-5; HA5; hHa5; K35; KRTHA5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_002280, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCTTCCAAATGCCTCAAGGCCGGCTTCTCTTCTGGGTCTCTCAAGAGCCCAGGAGGGGCCAGTGGGG
  GCTCCACTCGTGTGTCCGCAATGTACTCCAGCAGCTCTTGCAAGCTTCCAAGTCTCTCCCCTGTGGCCAG
  AAGTTTCTCTGCCTGCTCAGTGGGTCTGGGCAGAAGCAGCTACAGGGCCACCAGCTGCCTCCCTGCTCTC
  TGCCTCCCTGCTGGAGGCTTCGCTACCAGCTACAGTGGGGGTGGGGGCTGGTTTGGGGAGGGCATCCTCA
  CTGGCAATGAGAAGGAGACCATGCAATCCCTGAACGACCGCCTGGCCGGCTACCTGGAGAAGGTGCGTCA
  GCTGGAGCAGGAGAACGCCAGCCTGGAGAGCCGCATCCGTGAGTGGTGTGAGCAGCAGGTCCCCTACATG
  TGCCCTGACTACCAGTCCTACTTCCGGACCATCGAGGAGCTCCAGAAGAAGACTCTATGCAGCAAGGCTG
  AGAATGCCAGGCTGGTGGTGGAGATTGACAATGCCAAATTGGCTGCAGATGACTTCAGGACCAAGTATGA
  GACGGAGGTGTCCCTGCGGCAGCTGGTGGAGTCAGACATCAACGGCCTGCGCAGGATCCTGGATGACCTG
  ACCCTGTGCAAGTCTGACCTGGAGGCCCAGGTGGAGTCCCTGAAGGAGGAGCTGCTCTGCCTGAAGAAGA
  ACCATGAGGAGGAAGTGAACTCACTGCGCTGCCAACTTGGTGACCGCCTCAATGTTGAGGTGGACGCTGC
  CCCACCTGTTGACCTGAACCGAGTTCTGGAGGAGATGAGGTGCCAGTATGAAACCCTGGTGGAGAATAAC
  CGCCGGGATGCTGAAGACTGGTTGGACACCCAGAGTGAGGAGCTGAACCAGCAGGTGGTGTCCAGCTCAG
  AGCAGTTGCAGTCCTGCCAGGCAGAGATCATCGAGCTGAGACGCACGGTCAACGCCCTGGAGATTGAGCT
  GCAGGCTCAGCACAGCATGAGAGATGCTTTGGAATCCACCCTGGCAGAGACGGAGGCCCGCTATAGCTCC
  CAGCTGGCCCAGATGCAGTGCATGATCACCAACGTGGAGGCCCAGCTGGCCGAGATCCGGGCTGACCTGG
  AGCGGCAGAACCAGGAGTACCAGGTGCTGCTGGACGTCCGGGCCCGGCTGGAGTGTGAGATCAACACGTA
  CCGGGGCCTGCTGGAGAGTGAGGACAGCAAGCTCCCCTGTAACCCATGTGCACCTGACTACTCACCCTCC
  AAGTCATGCCTTCCCTGTCTTCCTGCGGCCTCCTGCGGTCCTAGTGCAGCCCGCACAAACTGCAGCCCCC
  GCCCCATTTGTGTGCCCTGCCCAGGGGGTCGGTTCTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_002280
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002280.4, NP_002271.3
• RefSeq Size: 1688 bp
• RefSeq ORF: 1368 bp
• Locus ID: 3886
• Cytogenetics: 17q21.2
• Gene Summary: 'The protein encoded by this gene is a member of the keratin gene family. This type I hair keratin is an acidic protein which heterodimerizes with type II keratins to form hair and nails. The type I hair keratins are clustered in a region of chromosome 17q12-q21 and have the same direction of transcription. [provided by RefSeq, Jul 2008]'