FUCA1 (NM_000147) Human Untagged Clone

CAT#: SC317765

FUCA1 (untagged)-Human fucosidase, alpha-L- 1, tissue (FUCA1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FUCA1
• Synonyms: FUCA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000147, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCGGGCTCCGGGGATGAGGTCGCGGCCGGCGGGTCCCGCGCTGTTGCTGCTGCTGCTCTTCCTCGGAG
  CGGCCGAGTCGGTGCGTCGGGCCCAGCCTCCGCGCCGCTACACCCCAGACTGGCCGAGCCTGGATTCTCG
  GCCGCTGCCGGCCTGGTTCGACGAAGCCAAGTTCGGGGTGTTCATCCACTGGGGCGTGTTCTCGGTGCCC
  GCCTGGGGCAGCGAGTGGTTCTGGTGGCACTGGCAGGGCGAGGGGCGGCCGCAGTACCAGCGCTTCATGC
  GCGACAACTACCCGCCCGGCTTCAGCTACGCCGACTTCGGACCGCAGTTCACTGCGCGCTTCTTCCACCC
  GGAGGAGTGGGCCGACCTCTTCCAGGCCGCGGGCGCCAAGTATGTAGTTTTGACGACAAAGCATCACGAA
  GGCTTCACAAACTGGCCGAGTCCTGTGTCTTGGAACTGGAACTCCAAAGACGTGGGGCCTCATCGGGATT
  TGGTTGGTGAATTGGGAACAGCTCTCCGGAAGAGGAACATCCGCTATGGACTATACCACTCACTCTTAGA
  GTGGTTCCATCCACTCTATCTACTTGATAAGAAAAATGGCTTCAAAACACAGCATTTTGTCAGTGCAAAA
  ACAATGCCAGAGCTGTACGACCTTGTTAACAGCTATAAACCTGATCTGATCTGGTCTGATGGGGAGTGGG
  AATGTCCTGATACTTACTGGAACTCCACAAATTTTCTTTCATGGCTCTACAATGACAGCCCTGTCAAGGA
  TGAGGTGGTAGTAAATGACCGATGGGGTCAGAACTGTTCCTGTCACCATGGAGGATACTATAACTGTGAA
  GATAAATTCAAGCCACAGAGCTTGCCAGATCACAAGTGGGAGATGTGCACCAGCATTGACAAGTTTTCCT
  GGGGCTATCGTCGTGACATGGCATTGTCTGATGTTACAGAAGAATCTGAAATCATTTCGGAACTGGTTCA
  GACAGTAAGTTTGGGAGGCAACTATCTTCTGAACATTGGACCAACTAAAGATGGACTGATTGTTCCCATC
  TTCCAAGAAAGGCTTCTTGCTGTTGGGAAATGGCTGAGCATCAATGGGGAGGCTATCTATGCCTCCAAAC
  CATGGCGGGTGCAATGGGAAAAGAACACAACATCTGTATGGTATACCTCAAAGGGATCGGCTGTTTATGC
  CATTTTTCTGCACTGGCCAGAAAATGGAGTCTTAAACCTTGAATCCCCCATAACTACCTCAACTACAAAG
  ATAACAATGCTGGGAATTCAAGGAGATCTGAAGTGGTCCACAGATCCAGATAAAGGTCTCTTCATCTCTC
  TACCCCAGTTGCCACCCTCTGCTGTCCCCGCAGAGTTTGCTTGGACTATAAAGCTGACAGGAGTGAAGTA
  A
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_000147
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000147.4, NP_000138.2
• RefSeq Size: 2133 bp
• RefSeq ORF: 1401 bp
• Locus ID: 2517
• Cytogenetics: 1p36.11
• Domains: Alpha_L_fucos
• Protein Families: Druggable Genome
• Protein Pathways: Lysosome, Other glycan degradation
• Gene Summary: 'The protein encoded by this gene is a lysosomal enzyme involved in the degradation of fucose-containing glycoproteins and glycolipids. Mutations in this gene are associated with fucosidosis (FUCA1D), which is an autosomal recessive lysosomal storage disease. A pseudogene of this locus is present on chr 2.[provided by RefSeq, Oct 2009]'