PROX1 (NM_002763) Human Untagged Clone

CAT#: SC318217

PROX1 (untagged)-Human prospero homeobox 1 (PROX1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PROX1
• Synonyms: prospero-related homeobox 1; prospero homeobox 1
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002763 edited
  GAATCGGCACGAGGATAATACACCATTGCAGCCGGGGAAAGCAGAGCGGCGCAAAAGAGC
  TCTCGCCGGGTCCGCCTGCTCCCTCTCCGCTTCGCTCCTCTTCTCTTCTTTACCCTTCTC
  CTCTCTCCTCCTCTGCTGCTCTCTCCTCTCCTCCGCTCTTCACTCTCCTCCTCTCCTGCT
  CTCTCCTCTTCCCTTAGCTCCTCTTCTTTTCTTCTCCTCTTCTTCCCTCTCCTCGCCTCT
  CCCCTGCTCCTCTTCTCTCGTCTCCCCTCCCCTCCCGCCTCTCTCTCCCCTCTCCCTCTC
  CCACTCGCCCCGCTCGCTCGCTCGCTGTCGCACAGACTCACCGTCCCTTGTCCAATTATC
  ATATTCATCACCCGCAAGATATCACCGTGTGTGCACTCGCGTGTTTTCCTCTCTCTGCCG
  GGGGAAAAAAAAAGAGAGAGAGAGATAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGGCTC
  GGTCCCACTGCTCCCTACACCGCGGTCCCGGGATTCTTGAGCTGTGCCCAGCTGACGAGC
  TTTTGAAGATGGCACAATAACCGTCCAGTGATGCCTGACCATGACAGCACAGCCCTCTTA
  AGCCGGCAAACCAAGAGGAGAAGAGTTGACATTGGAGTGAAAAGGACGGTAGGGACAGCA
  TCTGCATTTTTTGCTAAGGCAAGAGCAACGTTTTTTAGTGCCATGAATCCCCAAGGTTCT
  GAGCAGGATGTTGAGTATTCAGTGGTGCAGCATGCAGATGGGGAAAAGTCAAATGTACTC
  CGCAAGCTGCTGAAGAGGGCGAACTCGTATGAAGATGCCATGATGCCTTTTCCAGGAGCA
  ACCATAATTTCCCAGCTGTTGAAAAATAACATGAACAAAAATGGTGGCACGGAGCCCAGT
  TTCCAAGCCAGCGGTCTCTCTAGTACAGGCTCCGAAGTACATCAGGAGGATATATGCAGC
  AACTCTTCAAGAGACAGCCCCCCAGAGTGTCTTTCCCCTTTTGGCAGGCCTACTATGAGC
  CAGTTTGATATGGATCGCTTATGTGATGAGCACCTGAGAGCAAAGCGCGCCCGGGTTGAG
  AATATAATTCGGGGTATGAGCCATTCCCCCAGTGTGGCATTAAGGGGCAATGAAAATGAA
  AGAGAGATGGCCCCGCAGTCTGTGAGTCCCCGAGAAAGTTACAGAGAAAACAAACGCAAG
  CAAAAGCTTCCCCAGCAGCAGCAACAGAGTTTCCAGCAGCTGGTTTCAGCCCGAAAAGAA
  CAGAAGCGAGAGGAGCGCCGACAGCTGAAACAGCAGCTGGAGGACATGCAGAAACAGCTG
  CGCCAGCTGCAGGAAAAGTTCTACCAAATCTATGACAGCACTGATTCGGAAAATGATGAA
  GATGGTAACCTGTCTGAAGACAGCATGCGCTCGGAGATCCTGGATGCCAGGGCCCAGGAC
  TCTGTCGGAAGGTCAGATAATGAGATGTGCGAGCTAGACCCAGGACAGTTTATTGACCGA
  GCTCGAGCCCTGATCAGAGAGCAGGAAATGGCTGAAAACAAGCCGAAGCGAGAAGGCAAC
  AACAAAGAAAGAGACCATGGGCCAAACTCCTTACAACCGGAAGGCAAACATTTGGCTGAG
  ACCTTGAAACAGGAACTGAACACTGCCATGTCGCAAGTTGTGGACACTGTGGTCAAAGTC
  TTTTCGGCCAAGCCCTCCCGCCAGGTTCCTCAGGTCTTCCCACCTCTCCAGATCCCCCAG
  GCCAGATTTGCAGTCAATGGGGAAAACCACAATTTCCACACCGCCAACCAGCGCCTGCAG
  TGCTTTGGCGACGTCATCATTCCGAACCCCCTGGACACCTTTGGCAATGTGCAGATGGCC
  AGTTCCACTGACCAGACAGAAGCACTGCCCCTGGTTGTCCGCAAAAACTCCTCTGACCAG
  TCTGCCTCCGGCCCTGCCGCTGGCGGCCACCACCAGCCCCTGCACCAGTCGCCTCTCTCT
  GCCACCACGGGCTTCACCACGTCCACCTTCCGCCACCCCTTCCCCCTTCCCTTGATGGCC
  TATCCATTTCAGAGCCCATTAGGTGCTCCCTCCGGCTCCTTCTCTGGAAAAGACAGAGCC
  TCTCCTGAATCCTTAGACTTAACTAGGGATACCACGAGTCTGAGGACCAAGATGTCATCT
  CACCACCTGAGCCACCACCCTTGTTCACCAGCACACCCGCCCAGCACCGCCGAAGGGCTC
  TCCTTGTCGCTCATAAAGTCCGAGTGCGGCGATCTTCAAGATATGTCTGAAATATCACCT
  TATTCGGGAAGTGCAATGCAGGAAGGATTGTCACCCAATCACTTGAAAAAAGCAAAGCTC
  ATGTTTTTTTATACCCGTTATCCCAGCTCCAATATGCTGAAGACCTACTTCTCCGACGTA
  AAGTTCAACAGATGCATTACCTCTCAGCTCATCAAGTGGTTTAGCAATTTCCGTGAGTTT
  TACTACATTCAGATGGAGAAGTACGCACGTCAAGCCATCAACGATGGGGTCACCAGTACT
  GAAGAGCTGTCTATAACCAGAGACTGTGAGCTGTACAGGGCTCTGAACATGCACTACAAT
  AAAGCAAATGACTTTGAGGTTCCAGAGAGATTCCTGGAAGTTGCTCAGATCACATTACGG
  GAGTTTTTCAATGCCATTATCGCAGGCAAAGATGTTGATCCTTCCTGGAAGAAGGCCATA
  TACAAGGTCATCTGCAAGCTGGATAGTGAAGTCCCTGAGATTTTCAAATCCCCGAACTGC
  CTACAAGAGCTGCTTCATGAGTAGAAATTTCAACAACTCTTTTTGAATGTATGAAGAGTA
  GCAGTCCCCTTTGGATGTCCAAGTTATATGTGTCTAGATTTTGATTTCATATATATGTGT
  ATGGGAGGCATGGATATGTTATGAAATCAGCTGGTAATTCCTCCTCATCACGTTTCTCTC
  ATTTTCTTTTGTTTTCCATTGCAAGGGGATGGTTGTTTTCTTTCTGCCTTTAGTTTGCTT
  TTGCCCAAGGCCCTTAACATTTGGACACTTAAAATAGGGTTAATTTTCAGGGAAAAAGAA
  TGTTGGCGTGTGTAAAGTCTCTATTAGCAATGAAGGGAATTTGTTAACGATGCATCCACT
  TGATTGATGACTTATTGCAAATGGCGGTTGGCTGAGGAAAACCCATGACACAGCACAACT
  CTACAGACAGTGATGTGTCTCTTGTTTCTACTGCTAAGAAGGTCTGAAAATTTAATGAAA
  CCACTTCATACATTTAAGTATTTTGTTTGGTTTGAACTCAATCAGTAGCTTTTCCTTACA
  TGTTTAAAAATAATTCCAATGACAGATGAGCAGCTCACTTTTCCAAAGTACCCCAAAAGG
  CCAAATTAAAAAAGAAAAATAATCACTCTCAAGCCTTGTCTAAGAAAAGAGGCAAACTCT
  GAAAGTCGTACCAGTTTCTTCTGGAGGCAAAGCAATTTTGCACAAAACCAGCTCTCTCAA
  GATGAGACTAGAAATTCATACCTGGTCTTGTAGCCACCTCTCTAAACTTGAAAATAGGTT
  CTTCTTCATAAGTGAGCTTACATCATTCTTCATAAAGAAAAATCCTATAACTTGTTATCA
  TTTTTGCTTCAGATACTAAAAGGCACTAAGTTTCCAATTTACGCTGCTCAACTTTGTTTA
  TATGCTTAAAAGGATTCTGTTTACTTAACAATTTTTTCCCCTAAAATACTATTTTCTGAA
  TACTTCCTTCCAGTAAGGAATAAAGGAAAGCCCAACTTGGCCATAAAATTCTTGCCTACA
  CTAGAAGTTTGTTGACAGCCATTAGCTGACTTGATCGTCATCTCCTAAGAGGAACACATA
  TATTTTCACAAGCAATTCCACACTATCCTGATGGGTATGCAAAGTGGTGACAGTCTAACT
  CAGTGTTTCTTCATTTTAGGTATAACATTTTAAAGCAATTGATAATGCCTCTTCCAATTC
  AXXXXXXXXXXXXXXXXXXXXXGGGCACCAGCCACCTCACTCTGCACCCGCGGCCTCACA
  CATCTCCCAGCTCACACTCTACTAATGCACAGAGTCATTAGATCCAATTTGTTATTTTTC
  TCACTTGCTTTAAAAAAAAGCAGTTTGGATAATCATGACATTGGAATAAAGTGGGAAGGA
  AAAATTCCATCAGCACAAAATAGGGAAGTAATCCCAACTTGTAGTCACAGTTTTCTGACT
  GGCTTTGTTTTAAAAGAGGATGGCAGTCCTTGTTCGTGTCAGTGTGCCACTGGGTTTTTG
  CTGTTCCGTGTAATTCATATCAACTTTGTGTTGCCATTTGCAAGGTAAAAGGCAAAGCTG
  TAGTGTATTCACCTATGTAGACAGATTGCTAGATATCTTTTTGATCTGGGGCGAGTTCAA
  TATTGATTCCAGACTTATTTGGATTTTTTTAGTATTATTTTCCCCTCCCTTTCTAATTTA
  AATAGACAAATTAAGCAAAAGTGTGTGTTCACAACCAAATGTTGATGCCCTTATCTACTG
  ATAATATCCTCTCAATGTTCACTGAGGCATAGAAATTATTTCAGAGTAGAAATTGCAGCA
  TGAGGATAAACTCACCTCTTTGTTCTGAAAATAGAACTTTATCACTATGCTTTCCGGTGG
  TTTTCCCTTTTACAATCGAAATCTTGTGCCTCCCAAGTGCATTGGAAAATGACAAAAGCC
  TGTCTCTCCAAATTCCTATTTAACAGTTTGATTTTTTTTTTTTAATCACCATCTTTCAAA
  TCTTAGCTCAACTCTCACCAAGTGAAAATTGGCTACTTGGGAGAAAGTTAACTTTCTATG
  GTGGGATGGTGAAGGATGAGGGACAGTTTACATAGGAAAAAAAAAAAAAAAAAACTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002763 unedited
  GGGAGGGAGAGGGGGANNAAAAGAAAAGTATAAAAACTAGTTTTTACACCCGCCCGTTGC
  CCCAAAGGGCGGTAGGCGTGTACGGTGGGNAGTCTATATAAGCAGNACTCATTTAGGTGA
  CACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGCGAATCGGCACGAGGATAA
  TACACCATTGCAGCCGGGGAAAGCAGAGCGGCGCAAAAGAGCTCTCGCCGGGTCCGCCTG
  CTCCCTCTCCGCTTCGCTCCTCTTCTCTTCTTTACCCTTCTCCTCTCTCCTCCTCTGCTG
  CTCTCTCCTCTCCTCCGCTCTTCTCTCTCCTCCTCTCCTGCTCTCTCCTCTTCCCTTAGC
  TCCTCTTCTTTTCTTCTCCTCTTCTTCCCTCTCCTCGCCTCTCCCCTGCTCCTCTTCTCT
  CGTCTCCCCTCCCCTCCCGCCTCTCTCTCCCCTCTCCCTCTCCCACTCGCCCCGCTCGCT
  CGCTCGCTGTCGCACAGACTCACCGTCCCTTGTCCAATTATCATATTCATCACCCGCAAG
  ATATCACCGTGTGTGCACTCGCGTGTTTTCCTCTCTCTGCCGGGGGAAAAAAAAAGAGAG
  AGAGAGATAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGGCTCGGTCCCACTGCTCCCTAC
  ACCGCGGTCCCGGGATTCTTGAGCTGTGCCCAGCTGACGAGCTTTTGAAGATGGCACAAT
  AACCGTCCAGTGATGCCTGACCATGACAGCACAGCCCTCTTAAGCCGGCANACCAAGAAG
  AGAAGAGTTGACATTGGAGTGAAAAGACGGTAGGGACAGCATCTGCATTTTTTGCCTAGG
  CAAGAAGCACGTTNTTTAGTGCCATGAATCCCCAAGCNTCTGAGCAGATGTGAGTATTCA
  GTGGTGCAGCATGCAN
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_002763 unedited
  CCTTCCTGGNAAAAAGGCCATATACAAGGTCATCTGCAAGCTGGATAGTGAAGTCCCTGA
  GATTTTCAAATCCCCGAACTGCCTACAAGAGCTGCTTCATGAGTAGAAATTTCAACAACT
  CTTTTTGAATGTATGAAGAGTAGCAGTCCCCTTTGGATGTCCAAGTTATATGTGTCTAGA
  TTTTGATTTCATATATATGTGTATGGGAGGCATGGATATGTTATGAAATCAGCTGGTAAT
  TCCTCCTCATCACGTTTCTCTCATTTTCTTTTGTTTTCCATTGCAAGGGGATGGTTGTTT
  TCTTTCTGCCTTTAGTTTGCTTTTGCCCAAGGCCCTTAACATTTGGACACTTAAAATAGG
  GTTAATTTTCAGGGAAAAAGAATGTTGGCGTGTGTAAAGTCTCTATTAGCAATGAAGGGA
  ATTTGTTAACGATGCATCCACTTGATTGATGACTTATTGCAAATGGCGGTTGGCTGAGGA
  AAACCCATGACACAGCACAACTCTACAGACAGTGATGTGTCTCTTGTTTCTACTGCTAAG
  AAGGTCTGAAAATTTAATGAAACCACTTCATACATTTAAGTATTTTGTTTGGTTTGAACT
  CAATCAGTAGCTTTTCCTTACATGTTTAAAAATAATTCCAATGACAGATGAGCAGCTCAC
  TTTTCCAAAGTACCCCAAAAGGCCAAATTAAAAAAGAAAAATAATCACTCTCAAGCCTTG
  TCTAAGAAAAGAGCANACTCTGAAAGTCGTACCAGTTTCTTCTGGAGGCAAAGCAATTTT
  GCACAAAACCAGCTCTCTCAAGATGAGACTAGAAATTCATACCTGTTCTTGTAGCCACCT
  CNTCTAAACTG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_002763
• Insert Size: 5500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002763.3, NP_002754.2
• RefSeq Size: 3088 bp
• RefSeq ORF: 2214 bp
• Locus ID: 5629
• Cytogenetics: 1q32.3
• Domains: Prox1
• Protein Families: Embryonic stem cells, ES Cell Differentiation/IPS
• Gene Summary: 'The protein encoded by this gene is a member of the homeobox transcription factor family. Members of this family contain a homeobox domain that consists of a 60-amino acid helix-turn-helix structure that binds DNA and RNA. The protein encoded by this gene is conserved across vertebrates and may play an essential role during development. Altered levels of this protein have been reported in cancers of different organs, such as colon, brain, blood, breast, pancreas, liver and esophagus. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2012]'
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.