DDX27 (NM_017895) Human Untagged Clone

CAT#: SC318280

DDX27 (untagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 (DDX27)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DDX27
• Synonyms: dJ686N3.1; DRS1; Drs1p; HSPC259; PP3241; RHLP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_017895, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGTACTTGCGCAAAGACGACGAGGAGGCTGCGAAAAGTTAAGGGCCGGACCGCAGGCTGTGCTCGCTT
  CCGGAAGTGGCTTCTGCGACAACATGCTTGCGGACCTCGGCTTAATCGGAACCATAGGCGAGGATGACGA
  GGTGCCGGTGGAGCCCGAGTCTGACTCCGGGGACGAGGAAGAGGAGGGGCCCATTGTGCTGGGCAGACGA
  CAAAAAGCTTTGGGGAAGAACCGCAGTGCTGATTTCAACCCTGATTTCGTTTTCACTGAGAAGGAGGGGA
  CGTACGATGGCAGCTGGGCCCTGGCTGATGTCATGAGCCAACTCAAGAAGAAGAGGGCAGCCACTACATT
  AGATGAGAAGATTGAGAAAGTTCGAAAGAAAAGGAAAACAGAGGATAAAGAAGCCAAGTCTGGGAAGTTG
  GAAAAGGAGAAAGAAGCAAAGGAAGGCTCTGAACCAAAGGAGCAGGAAGACCTTCAAGAGAATGATGAGG
  AAGGCTCAGAAGATGAAGCCTCGGAGACTGACTACTCATCAGCTGATGAGAACATCCTCACCAAAGCAGA
  TACACTCAAAGTAAAGGATCGGAAGAAGAAGAAGAAGAAAGGACAGGAAGCAGGAGGATTTTTTGAAGAT
  GCATCTCAGTACGATGAAAACCTCTCGTTCCAGGACATGAACCTTTCCCGCCCTCTTCTGAAGGCCATTA
  CAGCCATGGGCTTCAAGCAGCCCACCCCGATCCAGAAGGCGTGCATACCTGTGGGTCTATTGGGGAAGGA
  CATCTGTGCCTGTGCAGCCACTGGGACAGGTAAAACTGCCGCCTTTGCCCTGCCTGTTTTGGAGCGTCTG
  ATTTATAAACCCCGCCAGGCTCCAGTCACCCGCGTGCTGGTGCTAGTGCCCACCCGAGAGCTGGGCATCC
  AGGTGCACTCTGTCACCAGACAGCTGGCCCAGTTCTGCAACATCACCACCTGCCTGGCTGTGGGCGGCTT
  GGATGTGAAGTCTCAGGAAGCAGCTCTTCGGGCAGCGCCTGACATCCTCATCGCCACCCCAGGCCGGCTC
  ATCGATCACCTCCACAACTGCCCTTCCTTCCACCTGAGCAGCATCGAGGTGCTCATCCTGGACGAGGCTG
  ACAGGATGCTGGATGAGTACTTTGAGGAGCAGATGAAGGAGATCATCCGAATGTGTTCCCACCACCGCCA
  GACCATGCTCTTCTCGGCCACCATGACAGACGAGGTGAAAGATCTGGCTTCTGTCTCCTTGAAGAATCCT
  GTCCGGATATTTGTGAACAGCAACACAGATGTGGCTCCCTTCCTGCGGCAGGAGTTCATCCGGATCCGGC
  CTAATCGTGAAGGAGACCGGGAAGCCATCGTGGCAGCTTTGTTGACGAGGACCTTCACTGACCATGTGAT
  GCTGTTCACGCAAACCAAGAAGCAGGCCCACCGCATGCACATCCTCCTGGGGCTCATGGGGCTGCAGGTG
  GGTGAGCTCCATGGCAACTTGTCACAGACGCAGCGGCTGGAGGCCCTCCGGCGTTTTAAGGATGAACAGA
  TTGACATCCTCGTGGCCACTGATGTGGCAGCCCGTGGACTTGACATTGAGGGGGTCAAAACGGTAATCAA
  CTTCACAATGCCTAATACCATCAAACATTATGTCCACCGGGTGGGGCGAACAGCACGTGCTGGCAGGGCT
  GGGCGCTCAGTCTCTCTGGTGGGAGAAGATGAGCGGAAGATGCTGAAGGAGATTGTAAAAGCTGCCAAGG
  CCCCTGTGAAGGCCAGGATACTTCCCCAAGATGTCATCCTCAAATTCCGGGACAAGATTGAGAAAATGGA
  GAAAGATGTGTATGCAGTTCTGCAGCTAGAGGCGGAGGAAAAAGAGATGCAGCAGTCAGAAGCCCAGATC
  AATACAGCAAAGCGGCTCCTGGAGAAGGGGAAGGAGGCAGTGGTCCAAGAGCCCGAGAGGAGCTGGTTCC
  AGACCAAAGAAGAGAGGAAGAAGGAGAAAATTGCCAAAGCTCTGCAGGAATTTGACTTGGCCTTAAGAGG
  AAAGAAGAAAAGGAAGAAGTTTATGAAGGATGCCAAAAAAAAGGGGGAGATGACAGCAGAGGAAAGGTCT
  CAGTTTGAAATCCTCAAGGCGCAGATGTTTGCTGAACGGCTAGCGAAGAGGAATCGCAGAGCCAAGCGGG
  CCCGAGCAATGCCCGAGGAGGAGCCAGTGAGAGGTCCTGCCAAGAAGCAAAAGCAGGGGAAGAAATCTGT
  ATTTGATGAAGAACTCACCAACACAAGCAAGAAGGCCCTGAAACAGTATCGAGCTGGCCCTTCCTTTGAA
  GAAAGGAAACAGTTGGGCTTGCCCCACCAGAGACGAGGAGGAAACTTTAAATCTAAATCCAGATACAAGA
  GGAGGAAGTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_017895
• ORF Size: 2391 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_017895.7, NP_060365.7
• RefSeq Size: 2711
• RefSeq ORF: 2391
• Locus ID: 55661
• Domains: DEAD, helicase_C
• Gene Summary: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein involved in the processing of 5.8S and 28S ribosomal RNAs. More specifically, the encoded protein localizes to the nucleolus, where it interacts with the PeBoW complex to ensure proper 3' end formation of 47S rRNA. [provided by RefSeq, Jan 2017]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.