DDX27 (NM_017895) Human Tagged ORF Clone

CAT#: RC219725

DDX27 (Myc-DDK-tagged)-Human DEAD (Asp-Glu-Ala-Asp) box polypeptide 27 (DDX27)

Product Images

Western blot validation of overexpression lysate (Cat# LY413477) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219725 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DDX27
• Synonyms: dJ686N3.1; DRS1; Drs1p; HSPC259; PP3241; RHLP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219725 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTACTTGCGCAAAGACGACGAGGAGGCTGCGAAAAGTTAAGGGCCGGACCGCAGGCTGTGCTCGCTT
  CCGGAAGTGGCTTCTGCGACAACATGCTTGCGGACCTCGGCTTAATCGGAACCATAGGCGAGGATGACGA
  GGTGCCGGTGGAGCCCGAGTCTGACTCCGGGGACGAGGAAGAGGAGGGGCCCATTGTGCTGGGCAGACGA
  CAAAAAGCTTTGGGGAAGAACCGCAGTGCTGATTTCAACCCTGATTTCGTTTTCACTGAGAAGGAGGGGA
  CGTACGATGGCAGCTGGGCCCTGGCTGATGTCATGAGCCAACTCAAGAAGAAGAGGGCAGCCACTACATT
  AGATGAGAAGATTGAGAAAGTTCGAAAGAAAAGGAAAACAGAGGATAAAGAAGCCAAGTCTGGGAAGTTG
  GAAAAGGAGAAAGAAGCAAAGGAAGGCTCTGAACCAAAGGAGCAGGAAGACCTTCAAGAGAATGATGAGG
  AAGGCTCAGAAGATGAAGCCTCGGAGACTGACTACTCATCAGCTGATGAGAACATCCTCACCAAAGCAGA
  TACACTCAAAGTAAAGGATCGGAAGAAGAAGAAGAAGAAAGGACAGGAAGCAGGAGGATTTTTTGAAGAT
  GCATCTCAGTACGATGAAAACCTCTCGTTCCAGGACATGAACCTTTCCCGCCCTCTTCTGAAGGCCATTA
  CAGCCATGGGCTTCAAGCAGCCCACCCCGATCCAGAAGGCGTGCATACCTGTGGGTCTATTGGGGAAGGA
  CATCTGTGCCTGTGCAGCCACTGGGACAGGTAAAACTGCCGCCTTTGCCCTGCCTGTTTTGGAGCGTCTG
  ATTTATAAACCCCGCCAGGCTCCAGTCACCCGCGTGCTGGTGCTAGTGCCCACCCGAGAGCTGGGCATCC
  AGGTGCACTCTGTCACCAGACAGCTGGCCCAGTTCTGCAACATCACCACCTGCCTGGCTGTGGGCGGCTT
  GGATGTGAAGTCTCAGGAAGCAGCTCTTCGGGCAGCGCCTGACATCCTCATCGCCACCCCAGGCCGGCTC
  ATCGATCACCTCCACAACTGCCCTTCCTTCCACCTGAGCAGCATCGAGGTGCTCATCCTGGACGAGGCTG
  ACAGGATGCTGGATGAGTACTTTGAGGAGCAGATGAAGGAGATCATCCGAATGTGTTCCCACCACCGCCA
  GACCATGCTCTTCTCGGCCACCATGACAGACGAGGTGAAAGATCTGGCTTCTGTCTCCTTGAAGAATCCT
  GTCCGGATATTTGTGAACAGCAACACAGATGTGGCTCCCTTCCTGCGGCAGGAGTTCATCCGGATCCGGC
  CTAATCGTGAAGGAGACCGGGAAGCCATCGTGGCAGCTTTGTTGACGAGGACCTTCACTGACCATGTGAT
  GCTGTTCACGCAAACCAAGAAGCAGGCCCACCGCATGCACATCCTCCTGGGGCTCATGGGGCTGCAGGTG
  GGTGAGCTCCATGGCAACTTGTCACAGACGCAGCGGCTGGAGGCCCTCCGGCGTTTTAAGGATGAACAGA
  TTGACATCCTCGTGGCCACTGATGTGGCAGCCCGTGGACTTGACATTGAGGGGGTCAAAACGGTAATCAA
  CTTCACAATGCCTAATACCATCAAACATTATGTCCACCGGGTGGGGCGAACAGCACGTGCTGGCAGGGCT
  GGGCGCTCAGTCTCTCTGGTGGGAGAAGATGAGCGGAAGATGCTGAAGGAGATTGTAAAAGCTGCCAAGG
  CCCCTGTGAAGGCCAGGATACTTCCCCAAGATGTCATCCTCAAATTCCGGGACAAGATTGAGAAAATGGA
  GAAAGATGTGTATGCAGTTCTGCAGCTAGAGGCGGAGGAAAAAGAGATGCAGCAGTCAGAAGCCCAGATC
  AATACAGCAAAGCGGCTCCTGGAGAAGGGGAAGGAGGCAGTGGTCCAAGAGCCCGAGAGGAGCTGGTTCC
  AGACCAAAGAAGAGAGGAAGAAGGAGAAAATTGCCAAAGCTCTGCAGGAATTTGACTTGGCCTTAAGAGG
  AAAGAAGAAAAGGAAGAAGTTTATGAAGGATGCCAAAAAAAAGGGGGAGATGACAGCAGAGGAAAGGTCT
  CAGTTTGAAATCCTCAAGGCGCAGATGTTTGCTGAACGGCTAGCGAAGAGGAATCGCAGAGCCAAGCGGG
  CCCGAGCAATGCCCGAGGAGGAGCCAGTGAGAGGTCCTGCCAAGAAGCAAAAGCAGGGGAAGAAATCTGT
  ATTTGATGAAGAACTCACCAACACAAGCAAGAAGGCCCTGAAACAGTATCGAGCTGGCCCTTCCTTTGAA
  GAAAGGAAACAGTTGGGCTTGCCCCACCAGAGACGAGGAGGAAACTTTAAATCTAAATCCAGATACAAGA
  GGAGGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219725 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MVLAQRRRGGCEKLRAGPQAVLASGSGFCDNMLADLGLIGTIGEDDEVPVEPESDSGDEEEEGPIVLGRR
  QKALGKNRSADFNPDFVFTEKEGTYDGSWALADVMSQLKKKRAATTLDEKIEKVRKKRKTEDKEAKSGKL
  EKEKEAKEGSEPKEQEDLQENDEEGSEDEASETDYSSADENILTKADTLKVKDRKKKKKKGQEAGGFFED
  ASQYDENLSFQDMNLSRPLLKAITAMGFKQPTPIQKACIPVGLLGKDICACAATGTGKTAAFALPVLERL
  IYKPRQAPVTRVLVLVPTRELGIQVHSVTRQLAQFCNITTCLAVGGLDVKSQEAALRAAPDILIATPGRL
  IDHLHNCPSFHLSSIEVLILDEADRMLDEYFEEQMKEIIRMCSHHRQTMLFSATMTDEVKDLASVSLKNP
  VRIFVNSNTDVAPFLRQEFIRIRPNREGDREAIVAALLTRTFTDHVMLFTQTKKQAHRMHILLGLMGLQV
  GELHGNLSQTQRLEALRRFKDEQIDILVATDVAARGLDIEGVKTVINFTMPNTIKHYVHRVGRTARAGRA
  GRSVSLVGEDERKMLKEIVKAAKAPVKARILPQDVILKFRDKIEKMEKDVYAVLQLEAEEKEMQQSEAQI
  NTAKRLLEKGKEAVVQEPERSWFQTKEERKKEKIAKALQEFDLALRGKKKRKKFMKDAKKKGEMTAEERS
  QFEILKAQMFAERLAKRNRRAKRARAMPEEEPVRGPAKKQKQGKKSVFDEELTNTSKKALKQYRAGPSFE
  ERKQLGLPHQRRGGNFKSKSRYKRRK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017895
• ORF Size: 2388 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_017895.6, NM_017895.7, NP_060365.6, NP_060365.7
• RefSeq Size: 2711 bp
• RefSeq ORF: 2391 bp
• Locus ID: 55661
• Domains: DEAD, helicase_C
• MW: 89.8 kDa
• Gene Summary: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein involved in the processing of 5.8S and 28S ribosomal RNAs. More specifically, the encoded protein localizes to the nucleolus, where it interacts with the PeBoW complex to ensure proper 3' end formation of 47S rRNA. [provided by RefSeq, Jan 2017]