PRDM9 (NM_020227) Human Untagged Clone

CAT#: SC318366

PRDM9 (untagged)-Human PR domain containing 9 (PRDM9)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PRDM9
• Synonyms: KMT8B; MEISETZ; MSBP3; PFM6; ZNF899
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_020227 edited
  ATGAGCCCTGAAAAGTCCCAAGAGGAGAGCCCAGAAGAAGACACAGAGAGAACAGAGCGG
  AAGCCCATGGTCAAAGATGCCTTCAAAGACATTTCCATATACTTCACCAAGGAAGAATGG
  GCAGAGATGGGAGACTGGGAGAAAACTCGCTATAGGAATGTGAAAAGGAACTATAATGCA
  CTGATTACTATAGGTCTCAGAGCCACTCGACCAGCTTTCATGTGTCACCGAAGGCAGGCC
  ATCAAACTCCAGGTGGATGACACAGAAGATTCTGATGAAGAATGGACCCCTAGGCAGCAA
  GTCAAACCTCCTTGGATGGCCTTAAGAGTGGAACAGCGTAAACACCAGAAGGGAATGCCC
  AAGGCGTCATTCAGTAATGAATCTAGTTTGAAAGAATTGTCAAGAACAGCAAATTTACTG
  AATGCAAGTGGCTCAGAGCAGGCTCAGAAACCAGTGTCCCCTTCTGGAGAAGCAAGTACC
  TCTGGACAGCACTCAAGACTAAAACTGGAACTCAGGAAGAAGGAGACTGAAAGAAAGATG
  TATAGCCTGCGAGAAAGAAAGGGTCATGCATACAAAGAGGTCAGCGAGCCGCAGGATGAT
  GATTACCTCTATTGTGAGATGTGTCAGAACTTCTTCATTGACAGCTGTGCTGCCCATGGG
  CCCCCTACATTTGTAAAGGACAGTGCAGTGGACAAGGGGCACCCCAACCGTTCAGCCCTC
  AGTCTGCCCCCAGGGCTGAGAATTGGGCCATCAGGCATCCCTCAGGCTGGGCTTGGAGTA
  TGGAATGAGGCATCTGATCTGCCGCTGGGTCTGCACTTTGGCCCTTATGAGGGCCGAATT
  ACAGAAGACGAAGAGGCAGCCAACAATGGATACTCCTGGCTGATCACCAAGGGGAGAAAC
  TGCTATGAGTATGTGGATGGAAAAGATAAATCCTGGGCCAACTGGATGAGGTATGTGAAC
  TGTGCCCGGGATGATGAAGAGCAGAACCTGGTGGCCTTCCAGTACCACAGGCAGATCTTC
  TATAGAACCTGCCGAGTCATTAGGCCAGGCTGTGAACTGCTGGTCTGGTATGGGGATGAA
  TACGGCCAGGAACTGGGCATCAAGTGGGGCAGCAAGTGGAAGAAAGAGCTCATGGCAGGG
  AGAGAACCAAAGCCAGAGATCCATCCATGTCCCTCATGCTGTCTGGCCTTTTCAAGTCAG
  AAATTTCTCAGTCAACATGTAGAACGCAATCACTCCTCTCAGAACTTCCCAGGACCATCT
  GCAAGAAAACTCCTCCAACCAGAGAATCCCTGCCCAGGGGATCAGAATCAGGAGCAGCAA
  TATCCAGATCCACACAGCCGTAATGACAAAACCAAAGGTCAAGAGATCAAAGAAAGGTCC
  AAACTCTTGAATAAAAGGACATGGCAGAGGGAGATTTCAAGGGCCTTTTCTAGCCCACCC
  AAAGGACAAATGGGGAGCTGTAGAGTGGGAAAAAGAATAATGGAAGAAGAGTCCAGAACA
  GGCCAGAAAGTGAATCCAGGGAACACAGGCAAATTATTTGTGGGGGTAGGAATCTCAAGA
  ATTGCAAAAGTCAAGTATGGAGAGTGTGGACAAGGTTTCAGTGTTAAATCAGATGTTATT
  ACACACCAAAGGACACATACAGGGGAGAAGCTCTACGTCTGCAGGGAGTGTGGGCGGGGC
  TTTAGCTGGAAGTCACACCTCCTCATTCACCAGAGGATACACACAGGGGAGAAGCCCTAT
  GTCTGCAGGGAGTGTGGGCGGGGCTTTAGCTGGCAGTCAGTCCTCCTCACTCACCAGAGG
  ACACACACAGGGGAGAAGCCCTATGTCTGCAGGGAGTGTGGGCGGGGCTTTAGCCGGCAG
  TCAGTCCTCCTCACTCACCAGAGGAGACACACAGGGGAGAAGCCCTATGTCTGCAGGGAG
  TGTGGGCGGGGCTTTAGCCGGCAGTCAGTCCTCCTCACTCACCAGAGGAGACACACAGGG
  GAGAAGCCCTATGTCTGCAGGGAGTGTGGGCGGGGCTTTAGCTGGCAGTCAGTCCTCCTC
  ACTCACCAGAGGACACACACAGGGGAGAAGCCCTATGTCTGCAGGGAGTGTGGGCGGGGC
  TTTAGCTGGCAGTCAGTCCTCCTCACTCACCAGAGGACACACACAGGGGAGAAGCCCTAT
  GTCTGCAGGGAGTGTGGGCGGGGCTTTAGCAATAAGTCACACCTCCTCAGACACCAGAGG
  ACACACACAGGGGAGAAGCCCTATGTCTGCAGGGAGTGTGGGCGGGGCTTTCGCGATAAG
  TCACACCTCCTCAGACACCAGAGGACACACACAGGGGAGAAGCCCTATGTCTGCAGGGAG
  TGTGGGCGGGGCTTTAGAGATAAGTCAAACCTCCTCAGTCACCAGAGGACACACACAGGG
  GAGAAGCCCTATGTCTGCAGGGAGTGTGGGCGGGGCTTTAGCAATAAGTCACACCTCCTC
  AGACACCAGAGGACACACACAGGGGAGAAGCCCTATGTCTGCAGGGAGTGTGGGCGGGGC
  TTTCGCAATAAGTCACACCTCCTCAGACACCAGAGGACACACACAGGGGAGAAGCCCTAC
  GTCTGCAGGGAGTGTGGGCGGGGCTTTAGCGATAGGTCAAGCCTCTGCTATCACCAGAGG
  ACACACACAGGGGAGAAGCCCTACGTCTGCAGGGAGGATGAGTAA
  
• Restriction Sites: Please inquire
• ACCN: NM_020227
• ORF Size: 2685 bp
• Insert Size: 3000
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_020227.2, NP_064612.2
• RefSeq Size: 3691
• RefSeq ORF: 2685
• Locus ID: 56979
• Gene Summary: The protein encoded by this gene is a zinc finger protein with histone methyltransferase activity that catalyzes histone H3 lysine 4 trimethylation (H3K4me3) during meiotic prophase. This protein contains multiple domains, including a Kruppel-associated box (KRAB) domain, an SSX repression domain (SSXRD), a PRD1-BF1 and RIZ homologous region, a subclass of SET (PR/SET) domain, and a tandem array of C2H2 zinc fingers. The zinc finger array recognizes a short sequence motif, leading to local H3K4me3, and meiotic recombination hotspot activity. The observed allelic variation alters the DNA-binding sequence specificity of the protein, resulting in distinct meiotic recombination hotspots amongst individuals and populations. Multiple alternate alleles of this gene have been described. [provided by RefSeq, Jul 2015]
  Transcript Variant: This variant (PRDM9 B allele, PMID:20044539) matches the reference genome sequence and encodes isoform PRDM9 B.