HCFC1 (NM_005334) Human Untagged Clone

CAT#: SC318702

HCFC1 (untagged)-Human host cell factor C1 (VP16-accessory protein) (HCFC1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HCFC1
• Synonyms: CFF; HCF; HCF-1; HCF1; HFC1; MRX3; PPP1R89; VCAF
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005334, the custom clone sequence may differ by one or more nucleotides
  ATGGCTTCGGCCGTGTCGCCCGCCAACTTGCCAGCGGTGCTTCTGCAGCCCCGCTGGAAG
  CGAGTGGTGGGCTGGTCGGGTCCGGTGCCACGGCCCCGCCACGGCCACCGCGCCGTGGCC
  ATCAAGGAGCTCATCGTGGTGTTTGGCGGCGGCAACGAGGGAATAGTGGACGAACTGCAC
  GTGTACAACACGGCAACCAACCAGTGGTTCATCCCAGCCGTGAGGGGGGACATTCCCCCT
  GGGTGTGCAGCCTATGGCTTCGTGTGTGACGGGACTCGCCTCCTGGTGTTTGGTGGGATG
  GTGGAGTATGGGAAATACAGCAATGACCTCTACGAACTCCAGGCGAGCCGGTGGGAGTGG
  AAGAGACTCAAAGCAAAGACGCCCAAAAACGGGCCCCCTCCGTGTCCTCGACTCGGGCAC
  AGCTTCTCCCTTGTGGGCAACAAATGCTACCTGTTTGGGGGTCTGGCCAATGATAGCGAG
  GACCCAAAGAACAACATTCCAAGGTACCTGAATGACTTATATATCCTGGAATTACGGCCA
  GGCTCTGGAGTGGTAGCCTGGGACATTCCCATCACTTACGGGGTCCTACCACCACCCCGG
  GAGTCACATACTGCCGTGGTCTACACCGAAAAAGACAATAAGAAGTCCAAGCTGGTGATC
  TACGGCGGGATGAGTGGCTGCAGGCTGGGGGACCTGTGGACCCTAGATATTGACACCCTG
  ACGTGGAATAAGCCCAGTCTCAGCGGGGTGGCGCCTCTTCCTCGCAGTCTCCACTCGGCA
  ACCACCATCGGAAATAAAATGTACGTGTTTGGTGGCTGGGTGCCTCTCGTCATGGATGAC
  GTCAAAGTGGCCACACACGAGAAGGAGTGGAAGTGTACCAACACGCTGGCTTGTCTCAAC
  CTGGATACCATGGCCTGGGAGACCATCCTGATGGATACACTGGAGGACAACATCCCCCGT
  GCTCGGGCTGGCCACTGCGCAGTCGCCATCAACACCCGCCTGTACATTTGGAGTGGGCGT
  GACGGCTACCGCAAGGCCTGGAACAACCAGGTCTGCTGCAAGGACCTCTGGTACCTAGAG
  ACAGAAAAGCCACCACCCCCAGCCCGAGTACAACTGGTACGCGCCAACACCAACTCCCTG
  GAGGTGAGCTGGGGGGCAGTGGCAACAGCCGACAGCTACCTTCTCCAGCTCCAGAAATAT
  GACATTCCTGCCACGGCTGCTACTGCCACCTCCCCTACACCCAATCCGGTCCCATCTGTG
  CCTGCCAACCCTCCCAAGAGCCCTGCCCCAGCAGCAGCCGCACCTGCTGTGCAGCCGCTG
  ACCCAAGTAGGCATCACGCTCCTGCCCCAGGCTGCCCCCGCACCCCCGACCACCACCACC
  ATCCAGGTCTTGCCAACGGTGCCTGGCAGCTCCATTTCTGTGCCCACCGCAGCCAGGACT
  CAAGGTGTCCCTGCTGTTCTCAAAGTGACCGGTCCTCAGGCTACAACAGGAACTCCATTG
  GTCACCATGCGACCTGCCAGCCAGGCTGGGAAAGCCCCTGTCACCGTGACCTCCCTTCCC
  GCCGGAGTGCGGATGGTTGTGCCAACACAGAGTGCCCAGGGAACGGTGATTGGCAGTAGC
  CCACAGATGAGTGGGATGGCCGCACTGGCCGCTGCGGCCGCTGCCACCCAGAAGATCCCC
  CCTTCCTCGGCACCCACGGTGCTGAGTGTCCCAGCGGGTACCACCATCGTGAAGACCATG
  GCTGTGACACCTGGCACTACCACCCTCCCAGCCACTGTGAAGGTGGCCTCCTCGCCAGTC
  ATGGTGAGCAACCCTGCCACTCGCATGCTGAAGACTGCAGCCGCCCAGGTGGGGACATCG
  GTTTCCTCCGCCACCAACACGTCTACCCGCCCTATCATCACAGTGCACAAGTCAGGCACT
  GTGACAGTGGCCCAGCAAGCCCAGGTGGTGACCACAGTTGTGGGCGGGGTCACCAAGACC
  ATCACCCTGGTGAAGAGCCCCATCTCTGTCCCAGGAGGCAGTGCTCTGATTTCCAATCTG
  GGCAAAGTGATGTCGGTGGTCCAGACCAAACCAGTTCAGACTTCAGCAGTCACAGGCCAG
  GCGTCCACGGGTCCTGTGACTCAGATCATCCAGACCAAAGGGCCCCTGCCAGCGGGAACA
  ATCCTGAAGCTGGTGACCTCAGCAGATGGCAAGCCCACCACCATCATCACTACCACGCAG
  GCCAGTGGGGCGGGGACCAAGCCCACCATCCTGGGCATCAGCAGCGTCTCCCCCAGTACC
  ACCAAGCCCGGCACGACCACCATCATCAAAACCATCCCCATGTCGGCCATCATCACCCAG
  GCGGGCGCCACGGGTGTGACCAGCAGTCCTGGCATCAAGTCCCCCATCACCATCATCACC
  ACCAAGGTGATGACTTCAGGAACTGGAGCACCTGCGAAAATCATCACTGCTGTCCCCAAA
  ATTGCCACTGGCCACGGGCAGCAGGGAGTGACCCAGGTGGTGCTTAAGGGGGCCCCGGGA
  CAGCCAGGCACCATCCTCCGCACTGTGCCCATGGGGGGTGTTCGCCTGGTCACACCCGTC
  ACCGTCTCCGCCGTCAAGCCAGCCGTCACCACGTTGGTTGTGAAAGGCACCACAGGTGTC
  ACGACCCTAGGCACAGTGACAGGCACCGTCTCCACCAGCCTTGCCGGGGCGGGGGGCCAC
  AGCACTAGTGCTTCCCTGGCCACGCCCATCACCACCTTGGGCACCATTGCCACCCTCTCA
  AGCCAGGTGATCAACCCCACTGCCATCACTGTGTCGGCCGCACAGACCACGCTGACAGCG
  GCAGGCGGGCTCACAACCCCAACCATCACCATGCAGCCCGTGTCCCAGCCCACCCAGGTA
  ACTCTGATCACGGCACCTAGTGGGGTGGAGGCCCAGCCTGTGCATGACCTCCCTGTGTCC
  ATTCTGGCCTCCCCGACTACAGAACAGCCCACCGCCACAGTTACCATCGCCGACTCAGGC
  CAGGGTGATGTGCAGCCTGGCACTGTCACCTTGGTGTGCTCCAACCCACCCTGTGAGACC
  CACGAGACTGGCACCACCAACACGGCCACCACTACTGTTGTGGCTAACCTTGGGGGACAC
  CCCCAGCCCACCCAAGTGCAGTTCGTCTGTGACAGACAGGAGGCAGCTGCTTCTCTTGTG
  ACCTCGACTGTGGGCCAGCAGAATGGTAGCGTGGTCCGAGTCTGTTCGAACCCGCCCTGC
  GAGACCCACGAGACGGGCACCACCAACACCGCCACCACCGCCACCTCCAACATGGCCGGG
  CAGCATGGCTGCTCAAACCCACCCTGCGAGACCCACGAGACGGGCACCACCAACACTGCC
  ACTACAGCCATGTCGAGCGTCGGCGCCAACCACCAGCGAGATGCCCGTCGGGCCTGTGCA
  GCTGGCACCCCTGCCGTGATCCGGATCAGTGTGGCCACTGGGGCGCTGGAGGCAGCCCAG
  GGCTCTAAGTCCCAGTGCCAAACCCGCCAGACCAGCGCGACCAGCACCACCATGACTGTG
  ATGGCCACCGGGGCCCCGTGCTCGGCCGGCCCACTCCTTGGGCCGAGCATGGCACGGGAG
  CCCGGGGGCCGCAGCCCTGCTTTTGTGCAGTTGGCCCCTCTGAGCAGCAAAGTCAGGCTG
  AGCAGCCCAAGCATTAAGGACCTTCCTGCGGGGCGCCACAGCCATGCGGTCAGCACCGCT
  GCCATGACCCGTTCCAGCGTGGGTGCTGGGGAGCCCCGCATGGCACCTGTGTGCGAGAGC
  CTCCAGGGTGGCTCGCCCAGCACCACAGTGACTGTGACAGCCCTGGAGGCACTGCTGTGC
  CCCTCGGCCACCGTGACCCAAGTCTGCTCCAACCCACCATGTGAGACCCACGAGACAGGC
  ACCACCAACACCGCCACTACCTCGAATGCAGGCAGCGCCCAGAGGGTGTGCTCCAACCCG
  CCATGCGAGACCCACGAGACGGGCACCACCCACACGGCCACCACCGCTACTTCAAACGGG
  GGCACGGGCCAGCCCGAGGGTGGGCAGCAGCCCCCTGCTGGTCGCCCCTGTGAGACACAC
  CAGACCACTTCCACTGGCACCACCATGTCGGTCAGCGTGGGTGCCCTGCTTCCCGACGCC
  ACTTCTTCCCACAGGACCGTGGAGTCTGGCCTAGAGGTGGCGGCGGCACCCAGCGTCACC
  CCCCAGGCTGGCACCGCGCTGCTGGCTCCTTTCCCAACACAGAGGGTGTGCTCCAACCCC
  CCCTGTGAGACCCACGAGACGGGCACCACTCACACGGCCACCACTGTCACTTCCAACATG
  AGTTCAAACCAAGACCCCCCACCTGCTGCCAGCGATCAGGGAGAGGTGGAGAGCACCCAG
  GGCGACAGCGTGAACATCACCAGCTCCAGTGCCATCACGACAACCGTGTCCTCCACACTG
  ACGCGGGCTGTGACCACCGTGACGCAGTCCACACCGGTCCCGGGCCCCTCTGTGCCGCCC
  CCAGAGGAACTCCAGGTGTCGCCAGGTCCTCGCCAGCAGCTGCCGCCACGGCAGCTTCTG
  CAGTCGGCTTCCACAGCCCTGATGGGGGAGTCCGCCGAGGTCCTGTCAGCCTCCCAGACC
  CCTGAGCTCCCGGCCGCCGTGGATCTGAGCAGCACAGGGGAGCCATCTTCGGGCCAGGAG
  TCTGCCGGCTCTGCGGTGGTGGCCACTGTGGTGGTCCAGCCACCCCCACCCACACAGTCC
  GAAGTAGACCAGTTATCACTTCCCCAAGAGCTAATGGCCGAGGCCCAAGCTGGCACCACC
  ACCCTCATGGTAACGGGGCTCACCCCCGAGGAGCTGGCAGTGACGGCTGCTGCAGAAGCA
  GCTGCCCAGGCCGCAGCCACGGAGGAAGCCCAGGCCCTGGCCATCCAGGCGGTGCTCCAG
  GCCGCGCAGCAGGCCGTCATGGGCACCGGCGAGCCCATGGACACCTCCGAGGCAGCAGCA
  ACCGTGACTCAGGCGGAGCTGGGGCACCTGTCGGCCGAGGGTCAGGAGGGCCAGGCCACC
  ACCATACCCATTGTGCTGACACAGCAGGAGCTGGCTGCCCTGGTGCAGCAGCAGCAGCTG
  CAGGAGGCCCAGGCCCAGCAGCAGCATCACCACCTCCCCACTGAGGCCCTGGCCCCTGCC
  GACAGTCTCAACGACCCAGCCATTGAGAGCAATTGCCTCAATGAGCTGGCCGGCACGGTC
  CCCAGCACTGTGGCGCTGCTGCCCTCAACGGCCACTGAGAGCCTGGCTCCATCCAACACA
  TTTGTGGCCCCCCAGCCGGTTGTGGTGGCCAGCCCAGCCAAGCTGCAGGCTGCAGCTACC
  CTGACCGAAGTGGCCAATGGCATCGAGTCCCTGGGTGTGAAGCCAGACCTGCCGCCCCCA
  CCCAGCAAAGCCCCCATGAAGAAGGAAAACCAGTGGTTTGATGTGGGAGTCATTAAGGGC
  ACCAATGTAATGGTGACACACTATTTCCTGCCACCAGATGATGCTGTCCCATCAGACGAT
  GATTTGGGCACCGTCCCTGACTATAACCAGCTGAAGAAGCAGGAGCTGCAGCCAGGCACA
  GCCTATAAGTTTCGTGTTGCCGGAATCAATGCCTGTGGCCGGGGGCCCTTCAGCGAAATC
  TCAGCCTTTAAGACGTGCCTGCCTGGTTTCCCAGGGGCCCCTTGTGCCATTAAAATCAGC
  AAAAGTCCGGATGGTGCTCACCTCACCTGGGAGCCACCCTCTGTGACCTCCGGCAAGATT
  ATCGAGTACTCCGTGTACCTGGCCATCCAGAGCTCACAGGCTGGGGGCGAGCTCAAGAGC
  TCCACCCCGGCCCAGCTGGCCTTCATGCGGGTGTACTGCGGGCCCAGCCCCTCCTGCCTG
  GTGCAGTCCTCCAGCCTTTCCAACGCCCACATCGACTACACCACCAAGCCCGCCATCATC
  TTCCGCATCGCCGCCCGCAATGAGAAGGGCTATGGCCCGGCCACACAAGTGAGGTGGCTG
  CAGGAAACCAGTAAAGACAGCTCTGGCACCAAGCCAGCCAACAAGCGGCCCATGTCCTCT
  CCAGAAATGAAATCTGCTCCAAAGAAATCTAAGGCCGATGGTCAG
  
• Restriction Sites: Please inquire
• ACCN: NM_005334
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005334.2, NP_005325.2
• RefSeq Size: 8436 bp
• RefSeq ORF: 6108 bp
• Locus ID: 3054
• Cytogenetics: Xq28
• Protein Families: Transcription Factors
• Gene Summary: 'This gene is a member of the host cell factor family and encodes a protein with five Kelch repeats, a fibronectin-like motif, and six HCF repeats, each of which contains a highly specific cleavage signal. This nuclear coactivator is proteolytically cleaved at one of the six possible sites, resulting in the creation of an N-terminal chain and the corresponding C-terminal chain. The final form of this protein consists of noncovalently bound N- and C-terminal chains. The protein is involved in control of the cell cycle and transcriptional regulation during herpes simplex virus infection. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq, Jul 2008]'