GABRA2 (NM_001114175) Human Untagged Clone

CAT#: SC318886

GABRA2 (untagged)-Human gamma-aminobutyric acid (GABA) A receptor, alpha 2 (GABRA2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GABRA2
• Synonyms: FLJ97076
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001114175, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAAGACAAAATTGAACATCTACAACATGCAGTTCCTGCTTTTTGTTTTCTTGGTGTGGGACCCTGCCA
  GGTTGGTGCTGGCTAACATCCAAGAAGATGAGGCTAAAAATAACATTACCATCTTTACGAGAATTCTTGA
  CAGACTTCTGGATGGTTACGATAATCGGCTTAGACCAGGACTGGGAGACAGTATTACTGAAGTCTTCACT
  AACATCTACGTGACCAGTTTTGGCCCTGTCTCAGATACAGATATGGAATATACAATTGATGTTTTCTTTC
  GACAAAAATGGAAAGATGAACGTTTAAAATTTAAAGGTCCTATGAATATCCTTCGACTAAACAATTTAAT
  GGCTAGCAAAATCTGGACTCCAGATACCTTTTTTCACAATGGGAAAAAATCAGTAGCTCATAATATGACA
  ATGCCAAATAAGTTGCTTCGAATTCAGGATGATGGGACTCTGCTGTATACCATGAGGCTTACAGTTCAAG
  CTGAATGCCCAATGCACTTGGAGGATTTCCCAATGGATGCTCATTCATGTCCTCTGAAATTTGGCAGCTA
  TGCATATACAACTTCAGAGGTCACTTATATTTGGACTTACAATGCATCTGATTCAGTACAGGTTGCTCCT
  GATGGCTCTAGGTTAAATCAATATGACCTGCTGGGCCAATCAATCGGAAAGGAGACAATTAAATCCAGTA
  CAGGTGAATATACTGTAATGACAGCTCATTTCCACCTGAAAAGAAAAATTGGGTATTTTGTGATTCAAAC
  CTATCTGCCTTGCATCATGACTGTCATTCTCTCCCAAGTTTCATTCTGGCTTAACAGAGAATCTGTGCCT
  GCAAGAACTGTGTTTGGAGTAACAACTGTCCTAACAATGACAACTCTAAGCATCAGTGCTCGGAATTCTC
  TCCCCAAAGTGGCTTATGCAACTGCCATGGACTGGTTTATTGCTGTTTGTTATGCATTTGTGTTCTCTGC
  CCTAATTGAATTTGCAACTGTTAATTACTTCACCAAAAGAGGATGGGCTTGGGATGGGAAGAGTGTAGTA
  AATGACAAGAAAAAAGAAAAGGCTTCCGTTATGATACAGAACAACGCTTATGCAGTGGCTGTTGCCAATT
  ATGCCCCGAATCTTTCAAAAGATCCAGTTCTCTCCACCATCTCCAAGAGTGCAACCACGCCAGAACCCAA
  CAAGAAGCCAGAAAACAAGCCAGCTGAAGCAAAGAAAACTTTCAACAGTGTTAGCAAAATTGACAGAATG
  TCCAGAATAGTTTTTCCAGTTTTGTTTGGTACCTTTAATTTAGTTTACTGGGCTACATATTTAAACAGAG
  AACCTGTATTAGGGGTCAGTCCTTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001114175
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001114175.1, NP_001107647.1
• RefSeq Size: 2784 bp
• RefSeq ORF: 1356 bp
• Locus ID: 2555
• Cytogenetics: 4p12
• Protein Families: Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• Gene Summary: 'GABA is the major inhibitory neurotransmitter in the mammalian brain where it acts at GABA-A receptors, which are ligand-gated chloride channels. Chloride conductance of these channels can be modulated by agents such as benzodiazepines that bind to the GABA-A receptor. At least 16 distinct subunits of GABA-A receptors have been identified. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]'
  Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Both variants 1 and 2 encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.