ENTPD6 (NM_001114089) Human Untagged Clone

CAT#: SC318897

ENTPD6 (untagged)-Human ectonucleoside triphosphate diphosphohydrolase 6 (putative) (ENTPD6), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ENTPD6
• Synonyms: CD39L2; dJ738P15.3; IL-6SAG; IL6ST2; NTPDase-6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001114089, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCAGCCGCAGCACGGTCCTTGGCAAACAAGGATGAGAAAAATATCCAACCACGGGAGCCTGCGGGTGG
  CGAAGGTGGCATACCCCCTGGGGCTGTGTGTGGGCGTGTTCATCTATGTTGCCTACATCAAGTGGCACCG
  GGCCACCGCCACCCAGGCCTTCTTCAGCATCACCAGGGCAGCCCCGGGGGCCCGGTGGGGTCAGCAGGCC
  CACAGCCCCCTGGGGACAGCTGCAGACGGGCACGAGGTCTTCTACGGGATCATGTTTGATGCAGGAAGCA
  CTGGCACCCGAGTACACGTCTTCCAGTTCACCCGGCCCCCCAGAGAAACTCCCACGTTAACCCACGAAAC
  CTTCAAAGCACTGAAGCCAGGTCTTTCTGCCTATGCTGATGATGTTGAAAAGAGCGCTCAGGGAATCCGG
  GAACTACTGGATGTTGCTAAACAGGACATTCCGTTCGACTTCTGGAAGGCCACCCCTCTGGTCCTCAAGG
  CCACAGCTGGCTTACGCCTGTTACCTGGAGAAAAGGCCCAGAAGTTACTGCAGAAGGTGAAAGAAGTATT
  TAAAGCATCGCCTTTCCTTGTAGGGGATGACTGTGTTTCCATCATGAACGGAACAGATGAAGGCGTTTCG
  GCGTGGATCACCATCAACTTCCTGACAGGCAGCTTGAAAACTCCAGGAGGGAGCAGCGTGGGCATGCTGG
  ACTTGGGCGGAGGATCCACTCAGATCGCCTTCCTGCCACGCGTGGAGGGCACCCTGCAGGCCTCCCCACC
  CGGCTACCTGACGGCACTGCGGATGTTTAACAGGACCTACAAGCTCTATTCCTACAGCTACCTCGGGCTC
  GGGCTGATGTCGGCACGCCTGGCGATCCTGGGCGGCGTGGAGGGGCAGCCTGCTAAGGATGGAAAGGAGT
  TGGTCAGCCCTTGCTTGTCTCCCAGTTTCAAAGGAGAGTGGGAACACGCAGAAGTCACGTACAGGGTTTC
  AGGGCAGAAAGCAGCGGCAAGCCTGCACGAGCTGTGTGCTGCCAGAGTGTCAGAGGTCCTTCAAAACAGA
  GTGCACAGGACGGAGGAAGTGAAGCATGTGGACTTCTATGCTTTCTCCTACTATTACGACCTTGCAGCTG
  GTGTGGGCCTCATAGATGCGGAGAAGGGAGGCAGCCTGGTGGTGGGGGACTTCGAGATCGCAGCCAAGTA
  CGTGTGTCGGACCCTGGAGACACAGCCGCAGAGCAGCCCCTTCTCATGCATGGACCTCACCTACGTCAGC
  CTGCTACTCCAGGAGTTCGGCTTTCCCAGGAGCAAAGTGCTGAAGCTCACTCGGAAAATTGACAATGTTG
  AGACCAGCTGGGCTCTGGGGGCCATTTTTCATTACATCGACTCCCTGAACAGACAGAAGAGTCCAGCCTC
  ATAG
  
  
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001114089
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001114089.3, NP_001107561.1
• RefSeq Size: 4092 bp
• RefSeq ORF: 1404 bp
• Locus ID: 955
• Cytogenetics: 20p11.21
• Protein Families: Secreted Protein, Transmembrane
• Protein Pathways: Purine metabolism, Pyrimidine metabolism
• Gene Summary: 'ENTPD6 is similar to E-type nucleotidases (NTPases). NTPases, such as CD39, mediate catabolism of extracellular nucleotides. ENTPD6 contains 4 apyrase-conserved regions which are characteristic of NTPases. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2015]'
  Transcript Variant: This variant (2) lacks an internal exon in the 5' region, resulting in a distinct 5' UTR and the use of an alternate start codon, compared to variant 1. The encoded isoform (2) has a distinct N-terminus, and is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.