Paxillin (PXN) (NM_001080855) Human Untagged Clone

CAT#: SC318942

PXN (untagged)-Human paxillin (PXN), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PXN
• Synonyms: FLJ16691
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001080855 edited
  ATGGACGACCTCGACGCCCTGCTGGCGGACTTGGAGTCTACCACCTCCCACATCTCCAAA
  CGGCCTGTGTTCTTGTCGGAGGAGACCCCCTACTCATACCCAACTGGAAACCACACATAC
  CAGGAGATTGCCGTGCCACCCCCCGTCCCCCCACCCCCGTCCAGCGAGGCCCTCAATGGC
  ACAATCCTTGACCCCTTAGACCAGTGGCAGCCCAGCGGCTCCCGATTCATCCACCAGCAG
  CCTCAGTCCTCATCACCTGTGTACGGCTCCAGTGCCAAAACTTCCAGTGTCTCCAACCCT
  CAGGACAGTGTTGGCTCTCCGTGCTCCCGAGTGGGTGAGGAGGAGCACGTCTACAGCTTC
  CCCAACAAGCAGAAATCAGCTGAGCCTTCACCCACCGTAATGAGCACGTCCCTGGGCAGC
  AACCTTTCTGAACTCGACCGCCTGCTGCTGGAACTGAACGCTGTACAGCATAACCCGCCA
  GGCTTCCCTGCAGATGAGGCCAACTCAAGCCCCCCGCTTCCTGGGGCCCTGAGCCCCCTC
  TATGGTGTCCCAGAGACTAACAGCCCCTTGGGAGGCAAAGCTGGGCCCCTGACGAAAGAG
  AAGCCTAAGCGGAATGGGGGCCGGGGCCTGGAGGACGTGCGGCCCAGTGTGGAGAGTCTC
  TTGGATGAACTGGAGAGCTCCGTGCCCAGCCCCGTCCCTGCCATCACTGTGAACCAGGGC
  GAGATGAGCAGCCCGCAGCGCGTCACCTCCACCCAACAGCAGACACGCATCTCGGCCTCC
  TCTGCCACCAGGGAGCTGGACGAGCTGATGGCTTCGCTGTCGGATTTCAAGATCCAGGGC
  CTGGAGCAAAGAGCGGATGGGGAGCGGTGCTGGGCGGCCGGCTGGCCTCGGGACGGCGGG
  CGGAGCAGCCCCGGAGGGCAGGACGAGGGAGGGTTCATGGCCCAGGGGAAGACAGGGAGC
  AGCTCACCCCCTGGGGGGCCCCCGAAGCCCGGGAGCCAGCTGGACAGCATGCTGGGGAGC
  CTGCAGTCTGACCTGAACAAGCTGGGGGTCGCCACAGTCGCCAAAGGAGTCTGCGGGGCC
  TGCAAGAAGCCCATCGCCGGGCAGGTTGTGACCGCCATGGGGAAGACGTGGCACCCCGAG
  CACTTCGTCTGCACCCACTGCCAGGAGGAGATCGGATCCCGGAACTTCTTCGAGCGGGAT
  GGACAGCCCTACTGTGAAAAGGACTACCACAACCTCTTCTCCCCGCGCTGCTACTACTGC
  AACGGCCCCATCCTGGATAAAGTGGTGACAGCCCTTGACCGGACGTGGCACCCTGAACAC
  TTCTTCTGTGCACAGTGTGGAGCCTTCTTTGGTCCCGAAGGGTTCCACGAGAAGGACGGC
  AAGGCCTACTGTCGCAAGGACTACTTCGACATGTTCGCACCCAAGTGTGGCGGCTGCGCC
  CGGGCCATCCTGGAGAACTATATCTCAGCCCTCAACACGCTGTGGCATCCTGAGTGCTTT
  GTGTGCCGGGAATGCTTCACGCCATTCGTGAACGGCAGCTTCTTCGAGCACGACGGGCAG
  CCCTACTGTGAGGTGCACTACCACGAGCGGCGCGGCTCGCTGTGTTCTGGCTGCCAGAAG
  CCCATCACCGGCCGCTGCATCACCGCCATGGCCAAGAAGTTCCACCCCGAGCACTTCGTC
  TGTGCCTTCTGCCTCAAGCAGCTCAACAAGGGCACCTTCAAGGAGCAGAACGACAAGCCT
  TACTGTCAGAACTGCTTCCTCAAGCTCTTCTGCTAG
  
• Restriction Sites: Please inquire
• ACCN: NM_001080855
• Insert Size: 3800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001080855.1, NP_001074324.1
• RefSeq Size: 3788 bp
• RefSeq ORF: 1776 bp
• Locus ID: 5829
• Cytogenetics: 12q24.23
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: Chemokine signaling pathway, Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, VEGF signaling pathway
• Gene Summary: 'This gene encodes a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Alternatively spliced transcript variants encoding different isoforms have been described for this gene. These isoforms exhibit different expression pattern, and have different biochemical, as well as physiological properties (PMID:9054445). [provided by RefSeq, Aug 2011]'
  Transcript Variant: This variant (2) has an additional in-frame coding exon compared to variant 1. This results in a longer isoform (2, also known as isoform beta) containing a 34 aa protein segment not found in isoform 1.