SLC6A12 (NM_001122847) Human Untagged Clone

CAT#: SC318954

SLC6A12 (untagged)-Human solute carrier family 6 (neurotransmitter transporter, betaine/GABA), member 12 (SLC6A12), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SLC6A12
• Synonyms: BGT-1; BGT1; GAT2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001122847, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGACGGGAAGGTGGCAGTGCAAGAGTGTGGGCCTCCTGCAGTCTCCTGGGTCCCCGAGGAGGGAGAGA
  AGTTGGACCAGGAAGACGAGGACCAGGTGAAGGATCGGGGCCAATGGACCAACAAGATGGAGTTTGTGCT
  GTCAGTGGCCGGGGAGATCATTGGGCTGGGCAATGTCTGGAGGTTTCCCTATCTCTGCTACAAAAACGGA
  GGTGGAGCCTTCTTCATCCCCTACTTCATCTTCTTCTTTGTCTGCGGCATCCCGGTGTTCTTCCTGGAGG
  TGGCGTTGGGCCAATACACCAGCCAAGGGAGTGTCACAGCCTGGAGGAAGATCTGCCCCCTCTTCCAGGG
  CATTGGTCTGGCATCTGTGGTCATCGAGTCATATTTGAATGTCTACTACATCATCATCCTTGCCTGGGCT
  CTCTTCTACCTGTTCAGCTCCTTCACTTCTGAGCTGCCCTGGACGACCTGCAACAACTTTTGGAACACAG
  AGCATTGCACGGACTTTCTGAACCACTCAGGAGCCGGCACAGTGACCCCATTTGAGAATTTTACCTCACC
  TGTCATGGAATTCTGGGAGAGACGAGTTCTGGGCATCACCTCGGGCATCCATGACCTGGGCTCCCTGCGC
  TGGGAGCTGGCCCTGTGCCTCCTGCTCGCCTGGGTCATCTGCTATTTCTGCATCTGGAAGGGGGTCAAGT
  CCACAGGCAAGGTGGTTTATTTCACAGCCACGTTTCCGTACCTGATGCTTGTCATTTTGCTGATCAGAGG
  TGTCACCCTTCCCGGAGCCTACCAGGGCATCATCTACTACTTGAAGCCAGATTTGTTCCGCCTCAAGGAC
  CCTCAGGTGTGGATGGATGCGGGCACCCAGATCTTCTTCTCCTTTGCCATCTGCCAGGGGTGCCTGACAG
  CCCTGGGCAGCTACAACAAGTATCACAACAACTGCTACAAGGACTGCATCGCCCTCTGCTTCCTGAACAG
  TGCCACCAGCTTTGTGGCTGGGTTTGTTGTCTTCTCCATCCTGGGCTTCATGTCCCAAGAGCAAGGGGTG
  CCCATTTCTGAAGTGGCCGAGTCAGGTCCTGGGCTGGCCTTCATCGCCTTCCCCAAGGCTGTGACTATGA
  TGCCCTTATCCCAGCTGTGGTCCTGCCTGTTCTTTATCATGCTCATATTCCTAGGGCTGGACAGCCAGTT
  TGTCTGTGTGGAGTGCCTGGTGACAGCCTCCATAGACATGTTCCCCAGGCAGCTCCGGAAGAGCGGGCGG
  CGCGAGCTCCTCATCCTCACCATCGCCGTCATGTGCTACCTGATAGGGCTTTTCCTGGTCACCGAGGGCG
  GGATGTACATCTTCCAGCTGTTTGACTACTATGCTTCCAGTGGCATATGCCTGCTGTTCCTGTCATTGTT
  TGAAGTGGTCTGCATAAGCTGGGTGTATGGGGCGGACCGTTTCTATGACAACATTGAGGACATGATTGGC
  TACCGGCCATGGCCCCTGGTGAAGATCTCCTGGCTCTTCCTGACCCCTGGACTTTGCCTGGCCACTTTCC
  TCTTCTCCTTGAGCAAGTACACCCCCCTCAAGTACAACAACGTCTATGTGTACCCGCCCTGGGGATACTC
  CATTGGCTGGTTCCTGGCTCTGTCCTCCATGGTCTGTGTCCCACTCTTCGTCGTCATCACCCTCCTGAAG
  ACTCGGGGTCCTTTCAGGAAGCGTCTGCGTCAGCTCATCACCCCTGACTCCAGTCTGCCACAGCCCAAGC
  AACATCCCTGCTTGGATGGCAGTGCTGGCCGGAACTTTGGGCCCTCCCCAACAAGGGAAGGACTGATAGC
  CGGGGAGAAGGAGACCCATTTGTAG
  
  
• Restriction Sites: Please inquire
• ACCN: NM_001122847
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001122847.1, NP_001116319.1
• RefSeq Size: 3153 bp
• RefSeq ORF: 1845 bp
• Locus ID: 6539
• Cytogenetics: 12p13.33
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: ''
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1, 2, 3 and 4 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.