ECE1 (NM_001113348) Human Untagged Clone

CAT#: SC318976

ECE1 (untagged)-Human endothelin converting enzyme 1 (ECE1), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ECE1
• Synonyms: ECE
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001113348, the custom clone sequence may differ by one or more nucleotides
  ATGATGTCGACGTACAAGCGGGCCACGCTGGACGAGGAGGACCTGGTGGACTCGCTCTCC
  GAGGGCGACGCATACCCCAACGGCCTGCAGGTGAACTTCCACAGCCCCCGGAGTGGCCAG
  AGGTGCTGGGCTGCACGGACCCAGGTGGAGAAGCGGCTGGTGGTGTTGGTGGTACTTCTG
  GCGGCAGGACTGGTGGCCTGCTTGGCAGCACTGGGCATCCAGTACCAGACAAGATCCCCC
  TCTGTGTGCCTGAGCGAAGCTTGTGTCTCAGTGACCAGCTCCATCTTGAGCTCCATGGAC
  CCCACAGTGGACCCCTGCCATGACTTCTTCAGCTACGCCTGTGGGGGCTGGATCAAGGCC
  AACCCAGTCCCTGATGGCCACTCACGCTGGGGGACCTTCAGCAACCTCTGGGAACACAAC
  CAAGCAATCATCAAGCACCTCCTCGAAAACTCCACGGCCAGCGTGAGCGAGGCAGAGAGA
  AAGGCGCAAGTATACTACCGTGCGTGCATGAACGAGACCAGGATCGAGGAGCTCAGGGCC
  AAACCTCTAATGGAGTTGATTGAGAGGCTCGGGGGCTGGAACATCACAGGTCCCTGGGCC
  AAGGACAACTTCCAGGACACCCTGCAGGTGGTCACCGCCCACTACCGCACCTCACCCTTC
  TTCTCTGTCTATGTCAGTGCCGATTCCAAGAACTCCAACAGCAACGTGATCCAGGTGGAC
  CAGTCTGGCCTGGGCTTGCCCTCGAGAGACTATTACCTGAACAAAACTGAAAACGAGAAG
  GTGCTGACCGGATATCTGAACTACATGGTCCAGCTGGGGAAGCTGCTGGGCGGCGGGGAC
  GAGGAGGCCATCCGGCCCCAGATGCAGCAGATCTTGGACTTTGAGACGGCACTGGCCAAC
  ATCACCATCCCACAGGAGAAGCGCCGTGATGAGGAGCTCATCTACCACAAAGTGACGGCA
  GCCGAGCTGCAGACCTTGGCACCCGCCATCAACTGGTTGCCTTTTCTCAACACCATCTTC
  TACCCCGTGGAGATCAATGAATCCGAGCCTATTGTGGTCTATGACAAGGAATACCTTGAG
  CAGATCTCCACTCTCATCAACACCACCGACAGATGCCTGCTCAACAACTACATGATCTGG
  AACCTGGTGCGGAAAACAAGCTCCTTCCTTGACCAGCGCTTTCAGGACGCCGATGAGAAG
  TTCATGGAAGTCATGTACGGGACCAAGAAGACCTGTCTTCCTCGCTGGAAGTTTTGCGTG
  AGTGACACAGAAAACAACCTGGGCTTTGCGTTGGGCCCCATGTTTGTCAAAGCAACCTTC
  GCCGAGGACAGCAAGAGCATAGCCACCGAGATCATCCTGGAGATTAAGAAGGCATTTGAG
  GAAAGCCTGAGCACCCTGAAGTGGATGGATGAGGAAACCCGAAAATCAGCCAAGGAAAAG
  GCCGATGCCATCTACAACATGATAGGATACCCCAACTTCATCATGGATCCCAAGGAGCTG
  GACAAAGTGTTTAATGACTACACTGCAGTTCCAGACCTCTACTTTGAAAATGCCATGCGG
  TTTTTCAACTTCTCATGGAGGGTCACTGCCGATCAGCTCAGGAAAGCCCCCAACAGAGAT
  CAGTGGAGCATGACCCCGCCCATGGTGAACGCCTACTACTCGCCCACCAAGAATGAGATT
  GTGTTTCCGGCCGGGATCCTGCAGGCACCATTCTACACACGCTCCTCACCCAAGGCCTTA
  AACTTTGGTGGCATAGGTGTCGTCGTGGGCCATGAGCTGACTCATGCTTTTGATGATCAA
  GGACGGGAGTATGACAAGGACGGGAACCTCCGGCCATGGTGGAAGAACTCATCCGTGGAG
  GCCTTCAAGCGTCAGACCGAGTGCATGGTAGAGCAGTACAGCAACTACAGCGTGAACGGG
  GAGCCGGTGAACGGGCGGCACACCCTGGGGGAGAACATCGCCGACAACGGGGGTCTCAAG
  GCGGCCTATCGGGCTTACCAGAACTGGGTGAAGAAGAACGGGGCTGAGCACTCGCTCCCC
  ACCCTGGGCCTCACCAATAACCAGCTCTTCTTCCTGGGCTTTGCACAGGTCTGGTGCTCC
  GTCCGCACACCTGAGAGCTCCCACGAAGGCCTCATCACCGATCCCCACAGCCCCTCTCGC
  TTCCGGGTCATCGGCTCCCTCTCCAATTCCAAGGAGTTCTCAGAACACTTCCGCTGCCCA
  CCTGGCTCACCCATGAACCCGCCTCACAAGTGCGAAGTCTGG
  
• Restriction Sites: Please inquire
• ACCN: NM_001113348
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001113348.1, NP_001106819.1
• RefSeq Size: 5154 bp
• RefSeq ORF: 2265 bp
• Locus ID: 1889
• Cytogenetics: 1p36.12
• Protein Families: Druggable Genome, Protease, Transmembrane
• Gene Summary: 'The protein encoded by this gene is involved in proteolytic processing of endothelin precursors to biologically active peptides. Mutations in this gene are associated with Hirschsprung disease, cardiac defects and autonomic dysfunction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Sep 2009]'
  Transcript Variant: This variant (4) differs in the 5' UTR and coding region, compared to variant 1, resulting in a shorter protein (isoform 4 also known as isoform c) that has a distinct N-terminus, compared to isoform 1.