Complement Component 6 (C6) (NM_001115131) Human Untagged Clone

CAT#: SC319008

C6 (untagged)-Human complement component 6 (C6), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: C6
• Synonyms: complement component 6; OTTHUMP00000120010
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001115131, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCAGACGCTCTGTCTTGTACTTCATCCTGCTGAATGCTCTGATCAACAAGGGCCAAGCCTGCTTCT
  GTGATCACTATGCATGGACTCAGTGGACCAGCTGCTCAAAAACTTGCAATTCTGGAACCCAGAGCAGACA
  CAGACAAATAGTAGTAGATAAGTACTACCAGGAAAACTTTTGTGAACAGATTTGCAGCAAGCAGGAGACT
  AGAGAATGTAACTGGCAAAGATGCCCCATCAACTGCCTCCTGGGAGATTTTGGACCATGGTCAGACTGTG
  ACCCTTGTATTGAAAAACAGTCTAAAGTTAGATCTGTCTTGCGTCCCAGTCAGTTTGGGGGACAGCCATG
  CACTGCGCCTCTGGTAGCCTTTCAACCATGCATTCCATCTAAGCTCTGCAAAATTGAAGAGGCTGACTGC
  AAGAATAAATTTCGCTGTGACAGTGGCCGCTGCATTGCCAGAAAGTTAGAATGCAATGGAGAAAATGACT
  GTGGAGACAATTCAGATGAAAGGGACTGTGGGAGGACAAAGGCAGTATGCACACGGAAGTATAATCCCAT
  CCCTAGTGTACAGTTGATGGGCAATGGGTTTCATTTTCTGGCAGGAGAGCCCAGAGGAGAAGTCCTTGAT
  AACTCTTTCACTGGAGGAATATGTAAAACTGTCAAAAGCAGTAGGACAAGTAATCCATACCGTGTTCCGG
  CCAATCTGGAAAATGTCGGCTTTGAGGTACAAACTGCAGAAGATGACTTGAAAACAGATTTCTACAAGGA
  TTTAACTTCTCTTGGACACAATGAAAATCAACAAGGCTCATTCTCAAGTCAGGGGGGGAGCTCTTTCAGT
  GTACCAATTTTTTATTCCTCAAAGAGAAGTGAAAATATCAACCATAATTCTGCCTTCAAACAAGCCATTC
  AAGCCTCTCACAAAAAGGATTCTAGTTTTATTAGGATCCATAAAGTGATGAAAGTCTTAAACTTCACAAC
  GAAAGCTAAAGATCTGCACCTTTCTGATGTCTTTTTGAAAGCACTTAACCATCTGCCTCTAGAATACAAC
  TCTGCTTTGTACAGCCGAATATTCGATGACTTTGGGACTCATTACTTCACCTCTGGCTCCCTGGGAGGCG
  TGTATGACCTTCTCTATCAGTTTAGCAGTGAGGAACTAAAGAACTCAGGTTTAACCGAGGAAGAAGCCAA
  ACACTGTGTCAGGATTGAAACAAAGAAACGCGTTTTATTTGCTAAGAAAACAAAAGTGGAACATAGGTGC
  ACCACCAACAAGCTGTCAGAGAAACATGAAGGTTCATTTATACAGGGAGCAGAGAAATCCATATCCCTGA
  TTCGAGGTGGAAGGAGTGAATATGGAGCAGCTTTGGCATGGGAGAAAGGGAGCTCTGGTCTGGAGGAGAA
  GACATTTTCTGAGTGGTTAGAATCAGTGAAGGAAAATCCTGCTGTGATTGACTTTGAGCTTGCCCCCATC
  GTGGACTTGGTAAGAAACATCCCCTGTGCAGTGACAAAACGGAACAACCTCAGGAAAGCTTTGCAAGAGT
  ATGCAGCCAAGTTCGATCCTTGCCAGTGTGCTCCATGCCCTAATAATGGCCGACCCACCCTCTCAGGGAC
  TGAATGTCTGTGTGTGTGTCAGAGTGGCACCTATGGTGAGAACTGTGAGAAACAGTCTCCAGATTATAAA
  TCCAATGCAGTAGACGGACAGTGGGGTTGTTGGTCTTCCTGGAGTACCTGTGATGCTACTTATAAGAGAT
  CGAGAACCCGAGAATGCAATAATCCTGCCCCCCAACGAGGAGGGAAACGCTGTGAGGGGGAGAAGCGACA
  AGAGGAAGACTGCACATTTTCAATCATGGAAAACAATGGACAACCATGTATCAATGATGACGAAGAAATG
  AAAGAGGTCGATCTTCCTGAGATAGAAGCAGATTCCGGGTGTCCTCAGCCAGTTCCTCCAGAAAATGGAT
  TTATCCGGAATGAAAAGCAACTATACTTGGTTGGAGAAGATGTTGAAATTTCATGCCTTACTGGCTTTGA
  AACTGTTGGATACCAGTACTTCAGATGCTTACCAGACGGGACCTGGAGACAAGGGGATGTGGAATGCCAA
  CGGACGGAGTGCATCAAGCCAGTTGTGCAGGAAGTCCTGACAATTACACCATTTCAGAGATTGTATAGAA
  TTGGTGAATCCATTGAGCTAACTTGCCCCAAAGGCTTTGTTGTTGCTGGGCCATCAAGGTACACATGCCA
  GGGGAATTCCTGGACACCACCCATTTCAAACTCTCTCACCTGTGAAAAAGATACTCTAACAAAATTAAAA
  GGCCATTGTCAGCTGGGACAGAAACAATCAGGATCTGAATGCATTTGTATGTCTCCAGAAGAAGACTGTA
  GCCATCATTCAGAAGATCTCTGTGTGTTTGACACAGACTCCAACGATTACTTTACTTCACCCGCTTGTAA
  GTTTTTGGCTGAGAAATGTTTAAATAATCAGCAACTCCATTTTCTACATATTGGTTCCTGCCAAGACGGC
  CGCCAGTTAGAATGGGGTCTTGAAAGGACAAGACTTTCATCCAACAGCACAAAGAAAGAATCCTGTGGCT
  ATGACACCTGCTATGACTGGGAAAAATGTTCAGCCTCCACTTCCAAATGTGTCTGCCTATTGCCCCCACA
  GTGCTTCAAGGGTGGAAACCAACTCTACTGTGTCAAAATGGGATCATCAACAAGTGAGAAAACATTGAAC
  ATCTGTGAAGTGGGAACTATAAGATGTGCAAACAGGAAGATGGAAATACTGCATCCTGGAAAGTGTTTGG
  CCTAG
  
  
• Restriction Sites: SgfI-RsrII
• ACCN: NM_001115131
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001115131.2, NP_001108603.2
• RefSeq Size: 3749 bp
• RefSeq ORF: 2805 bp
• Locus ID: 729
• Cytogenetics: 5p13.1
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Complement and coagulation cascades, Prion diseases, Systemic lupus erythematosus
• Gene Summary: 'This gene encodes a component of the complement cascade. The encoded protein is part of the membrane attack complex that can be incorporated into the cell membrane and cause cell lysis. Mutations in this gene are associated with complement component-6 deficiency. Transcript variants encoding the same protein have been described.[provided by RefSeq, Nov 2012]'
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 both encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.