HADHSC (HADH) (NM_005327) Human Untagged Clone

CAT#: SC319061

HADH (untagged)-Human hydroxyacyl-CoA dehydrogenase (HADH), nuclear gene encoding mitochondrial protein, transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HADH
• Synonyms: HAD; HADH1; HADHSC; HCDH; HHF4; MSCHAD; SCHAD
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005327.2 CCTCGCTGTCGCCGCCGCTGCCACACCATGGCCTTCGTCACCAGGCAGTTCATGCGTTCC
  GTGTCCTCCTCGTCCACCGCCTCGGCCTCGGCCAAGAAGATAATCGTCAAGCACGTGACG
  GTCATCGGCGGCGGGCTGATGGGCGCCGGCATTGCCCAGGTTGCTGCAGCAACTGGTCAC
  ACAGTAGTGTTGGTAGACCAGACAGAGGACATCCTGGCAAAATCCAAAAAGGGAATTGAG
  GAAAGCCTTAGGAAAGTGGCAAAGAAGAAGTTTGCAGAAAACCCTAAGGCCGGCGATGAA
  TTTGTGGAGAAGACCCTGAGCACCATAGCGACCAGCACGGATGCAGCCTCCGTTGTCCAC
  AGCACAGACTTGGTGGTGGAAGCCATCGTGGAGAATCTGAAGGTGAAAAACGAGCTCTTC
  AAAAGGCTGGACAAGTTTGCTGCTGAACATACAATCTTTGCCAGCAACACTTCCTCCTTG
  CAGATTACAAGCATAGCTAATGCCACCACCAGACAAGACCGATTCGCTGGCCTCCATTTC
  TTCAACCCAGTGCCTGTCATGAAACTTGTGGAGGTCATTAAAACACCAATGACCAGCCAG
  AAGACATTTGAATCTTTGGTAGACTTTAGCAAAGCCCTAGGAAAGCATCCTGTTTCTTGC
  AAGGACACTCCTGGGTTTATTGTGAACCGCCTCCTGGTTCCATACCTCATGGAAGCAATC
  AGGCTGTATGAACGAGGTGACGCATCCAAAGAAGACATTGACACTGCTATGAAATTAGGA
  GCCGGTTACCCCATGGGCCCATTTGAGCTTCTAGATTATGTCGGACTGGATACTACGAAG
  TTCATCGTGGATGGGTGGCATGAAATGGATGCAGAGAACCCATTACATCAGCCCAGCCCA
  TCCTTAAATAAGCTGGTAGCAGAGAACAAGTTCGGCAAGAAGACTGGAGAAGGATTTTAC
  AAATACAAGTGATGTGCAGCTTCTCCGGCTCTGAGAAGAACACCTGAGAGCGCTTTCCAG
  CCAGTGCCCCGAGTGCCTGTGGGAATGCTCTTTGGTCAGACATTCCCTCACACAGTACAG
  ATTAATAAATGTGCATTTTGATTGTAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_005327
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005327.2, NP_005318.2
• RefSeq Size: 1929 bp
• RefSeq ORF: 945 bp
• Locus ID: 3033
• Cytogenetics: 4q25
• Domains: 3HCDH, 3HCDH_N
• Protein Pathways: Butanoate metabolism, Fatty acid elongation in mitochondria, Fatty acid metabolism, Lysine degradation, Metabolic pathways, Tryptophan metabolism, Valine, leucine and isoleucine degradation
• Gene Summary: 'This gene is a member of the 3-hydroxyacyl-CoA dehydrogenase gene family. The encoded protein functions in the mitochondrial matrix to catalyze the oxidation of straight-chain 3-hydroxyacyl-CoAs as part of the beta-oxidation pathway. Its enzymatic activity is highest with medium-chain-length fatty acids. Mutations in this gene cause one form of familial hyperinsulinemic hypoglycemia. The human genome contains a related pseudogene of this gene on chromosome 15. [provided by RefSeq, May 2010]'
  Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.