NSE (ENO2) (NM_001975) Human Untagged Clone

CAT#: SC319063

ENO2 (untagged)-Human enolase 2 (gamma, neuronal) (ENO2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ENO2
• Synonyms: HEL-S-279; NSE
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001975.2 CAGCGCCGCCGTCGCCACCGCCACCGCCACCGCCACTACCACCGGCTGAGTCTGCAGTCC
  CGAGATCCCAGCCATCATGTCCATAGAGAAGATCTGGGCCCGGGAGATCCTGGACTCCCG
  CGGGAACCCCACAGTGGAGGTGGATCTCTATACTGCCAAAGGTCTTTTCCGGGCTGCAGT
  GCCCAGTGGAGCCTCTACGGGCATCTATGAGGCCCTGGAGCTGAGGGATGGAGACAAACA
  GCGTTACTTAGGCAAAGGTGTCCTGAAGGCAGTGGACCACATCAACTCCACCATCGCGCC
  AGCCCTCATCAGCTCAGGTCTCTCTGTGGTGGAGCAAGAGAAACTGGACAACCTGATGCT
  GGAGTTGGATGGGACTGAGAACAAATCCAAGTTTGGGGCCAATGCCATCCTGGGTGTGTC
  TCTGGCCGTGTGTAAGGCAGGGGCAGCTGAGCGGGAACTGCCCCTGTATCGCCACATTGC
  TCAGCTGGCCGGGAACTCAGACCTCATCCTGCCTGTGCCGGCCTTCAACGTGATCAATGG
  TGGCTCTCATGCTGGCAACAAGCTGGCCATGCAGGAGTTCATGATCCTCCCAGTGGGAGC
  TGAGAGCTTTCGGGATGCCATGCGACTAGGTGCAGAGGTCTACCATACACTCAAGGGAGT
  CATCAAGGACAAATACGGCAAGGATGCCACCAATGTGGGGGATGAAGGTGGCTTTGCCCC
  CAATATCCTGGAGAACAGTGAAGCCTTGGAGCTGGTGAAGGAAGCCATCGACAAGGCTGG
  CTACACGGAAAAGATCGTTATTGGCATGGATGTTGCTGCCTCAGAGTTTTATCGTGATGG
  CAAATATGACTTGGACTTCAAGTCTCCCACTGATCCTTCCCGATACATCACTGGGGACCA
  GCTGGGGGCACTCTACCAGGACTTTGTCAGGGACTATCCTGTGGTCTCCATTGAGGACCC
  ATTTGACCAGGATGATTGGGCTGCCTGGTCCAAGTTCACAGCCAATGTAGGGATCCAGAT
  TGTGGGTGATGACCTGACAGTGACCAACCCAAAACGTATTGAGCGGGCAGTGGAAGAAAA
  GGCCTGCAACTGTCTGCTGCTCAAGGTCAACCAGATCGGCTCTGTCACTGAAGCCATCCA
  AGCGTGCAAGCTGGCCCAGGAGAATGGCTGGGGGGTCATGGTGAGTCATCGCTCAGGAGA
  GACTGAGGACACATTCATTGCTGACCTGGTGGTGGGGCTGTGCACAGGCCAGATCAAGAC
  TGGTGCCCCGTGCCGTTCTGAACGTCTGGCTAAATACAACCAGCTCATGAGAATTGAGGA
  AGAGCTGGGGGATGAAGCTCGCTTTGCCGGACATAACTTCCGTAATCCCAGTGTGCTGTG
  ATTCCTCTGCTTGCCTGGAGACGTGGAACCTCTGTCTCATCCTCCTGGAACCTTGCTGTC
  CTGATCTGTGATAGTTCACCCCCTGAGATCCCCTGAGCCCCAGGGTGCCCAGAACTTCCC
  TGATTGACCTGCTCCGCTGCTCCTTGGCTTACCTGACCTCTTGCTGTCTCTGCTCGCCCT
  CCTTTCTGTGCCCTACTCATTGGGGTTCCGCACTTTCCACTTCTTCCTTTCTCTTTCTCT
  CTTCCCTCAGAAACTAGAAATGTGAATGAGGATTATTATAAAAGGGGGTCCGTGGAAGAA
  TGATCAGCATCTGTGATGGGAGCGTCAGGGTTGGTGTGCTGAGGTGTTAGAGAGGGACCA
  TGTGTCACTTGTGCTTTGCTCTTGTCCCACGTGTCTTCCACTTTGCATATGAGCCGTGAA
  CTGTGCATAGTGCTGGGATGGAGGGGAGTGTTGGGCATGTGATCACGCCTGGCTAATAAG
  GCTTTAGTGTATTTATTTATTTATTTATTTTATTTGTTTTTCATTCATCCCATTAATCAT
  TTCCCCATAACTCAATGGCCTAAAACTGGCCTGACTTGGGGGAACGATGTGTCTGTATTT
  CATGTGGCTGTAGATCCCAAGATGACTGGGGTGGGAGGTCTTGCTAGAATGGGAAGGGTC
  ATAGAAAGGGCCTTGACATCAGTTCCTTTGTGTGTACTCACTGAAGCCTGCGTTGGTCCA
  GAGCGGAGGCTGTGTGCCTGGGGGAGTTTTCCTCTATACATCTCTCCCCAACCCTAGGTT
  CCCTGTTCTTCCTCCAGCTGCACCAGAGCAACCTCTCACTCCCCATGCCACGTTCCACAG
  TTGCCACCACCTCTGTGGCATTGAAATGAGCACCTCCATTAAAGTCTGAATCAGTGCAAA
  AAAAAATAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_001975
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001975.2, NP_001966.1
• RefSeq Size: 2423 bp
• RefSeq ORF: 1305 bp
• Locus ID: 2026
• Cytogenetics: 12p13.31
• Domains: enolase
• Protein Pathways: Glycolysis / Gluconeogenesis, Metabolic pathways, RNA degradation
• Gene Summary: 'This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. [provided by RefSeq, Jul 2008]'