Clathrin light chain (CLTB) (NM_001834) Human Untagged Clone

CAT#: SC319160

CLTB (untagged)-Human clathrin, light chain B (CLTB), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CLTB
• Synonyms: LCB
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001834.2 GAAGTGCGGTCCGCGCCAAGCCCGTCCCCGCCGACGCCGGCTCCCCGCGGCTCGGGTGAC
  AGCGTCGCGGCCGCCGGACGCAGCGCGGGGCAGGCGCGGGCAGAGCCGAGCGCAGCGGAG
  GCTCCGGCGGAGGCGCGGGGAAAATGGCTGATGACTTTGGCTTCTTCTCGTCGTCGGAGA
  GCGGTGCCCCGGAGGCGGCGGAGGAGGACCCGGCGGCCGCCTTCCTGGCCCAGCAGGAGA
  GCGAGATTGCAGGCATAGAGAACGACGAGGGCTTCGGGGCACCTGCCGGCAGCCATGCGG
  CCCCCGCGCAGCCGGGCCCCACGAGTGGGGCTGGTTCTGAGGACATGGGGACCACAGTCA
  ATGGAGATGTGTTTCAGGAGGCCAACGGTCCTGCTGATGGCTACGCAGCCATTGCCCAGG
  CTGACAGGCTGACCCAGGAGCCTGAGAGCATCCGCAAGTGGCGAGAGGAGCAGAGGAAAC
  GGCTGCAAGAGCTGGATGCTGCATCTAAGGTCACGGAACAGGAATGGCGGGAGAAGGCCA
  AGAAGGACCTGGAGGAGTGGAACCAGCGCCAGAGTGAACAAGTAGAGAAGAACAAGATCA
  ACAACCGGGCATCCGAGGAGGCTTTCGTGAAGGAATCCAAGGAGGAGACCCCAGGCACAG
  AGTGGGAGAAGGTGGCCCAGCTATGTGACTTCAACCCCAAGAGCAGCAAGCAGTGCAAAG
  ATGTGTCCCGCCTGCGCTCGGTGCTCATGTCCCTGAAGCAGACGCCACTGTCCCGCTAGG
  TGCCTGCTAGGTGCATGGCCACAGAGCATGGGCTGGGCCTGGGCACAGGAGGAGCAGCTG
  CTTTGGTCGGGGTGGAGACTCGCAGCAGCTGCTACCCACAGCCTATTCCACTCCTCCCCA
  TCTCCAGGCGCTGGGAGGGGGGCCCTCACCCCGTCACGCCTCGCTCCCTCCTGGCCCTCT
  GGTCCAGCCCCTCACGCCTCCTCTCAGTCTACTCAATTGTGACTGTCCCTCCTGATGTAT
  TTTTTTTCTTGGCTTAAAGGGTGTGTTGTTGACTCTTTTTACACTTATTTATTATCATTC
  TCACTTCTCTGGAAGCCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_001834
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001834.2, NP_001825.1
• RefSeq Size: 1130 bp
• RefSeq ORF: 636 bp
• Locus ID: 1212
• Cytogenetics: 5q35.2
• Domains: Clathrin_lg_ch
• Protein Pathways: Endocytosis, Huntington's disease, Lysosome
• Gene Summary: 'Clathrin is a large, soluble protein composed of heavy and light chains. It functions as the main structural component of the lattice-type cytoplasmic face of coated pits and vesicles which entrap specific macromolecules during receptor-mediated endocytosis. This gene encodes one of two clathrin light chain proteins which are believed to function as regulatory elements. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) lacks an alternate in-frame exon compared to variant 2, resulting in a shorter isoform (a) compared to isoform b.