Ube1L (UBA7) (NM_003335) Human Untagged Clone

CAT#: SC319174

UBA7 (untagged)-Human ubiquitin-like modifier activating enzyme 7 (UBA7)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: UBA7
• Synonyms: D8; UBA1B; UBE1L; UBE2
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003335.2 GGCTACAGCAGGGCACTGGCCCCTACTGTCACCGCCACCTACACAAAGACCCTATCTCTG
  AGCGCTGCAGCCTACTGTTCAGCCCCAGGTTTGAGGATGGATGCCCTGGACGCTTCGAAG
  CTACTGGATGAGGAGCTGTATTCAAGACAGCTGTATGTGCTGGGCTCACCTGCCATGCAG
  AGGATTCAGGGAGCCAGGGTCCTGGTGTCAGGCCTGCAGGGCCTGGGGGCCGAGGTGGCC
  AAGAACTTGGTTCTGATGGGTGTGGGCAGCCTCACTCTGCATGATCCCCACCCCACCTGC
  TGGTCCGACCTGGCTGCCCAGTTTCTCCTCTCAGAGCAGGACTTGGAAAGGAGCAGAGCC
  GAGGCCTCTCAAGAGCTCTTGGCTCAGCTCAACAGAGCTGTCCAGGTCGTCGTGCACACG
  GGTGACATCACTGAGGACCTGCTGTTGGACTTCCAGGTGGTGGTGCTGACTGCTGCAAAG
  CTGGAGGAGCAGCTGAAGGTGGGCACCTTGTGTCATAAGCATGGAGTTTGCTTTCTGGCG
  GCTGACACCCGGGGCCTCGTGGGGCAGTTGTTCTGTGACTTTGGTGAGGACTTCACTGTG
  CAGGACCCCACAGAGGCAGAACCCCTGACAGCTGCCATCCAGCACATCTCCCAGGGCTCC
  CCTGGCATTCTCACTCTGAGGAAAGGGGCCAATACCCACTACTTCCGTGATGGAGACTTG
  GTGACTTTCTCGGGAATTGAGGGAATGGTTGAGCTCAACGACTGTGATCCCCGGTCTATC
  CACGTGCGGGAGGATGGGTCCCTGGAGATTGGAGACACAACAACTTTCTCTCGGTACTTG
  CGTGGTGGGGCTATCACTGAAGTCAAGAGACCCAAGACTGTGAGACATAAGTCCCTGGAC
  ACAGCCCTGCTCCAGCCCCATGTGGTGGCCCAGAGCTCCCAGGAAGTTCACCATGCCCAC
  TGCCTGCATCAGGCCTTCTGTGCACTGCACAAGTTCCAGCACCTCCATGGCCGGCCACCC
  CAGCCCTGGGATCCTGTTGATGCAGAGACTGTGGTGGGCCTGGCCCGGGACCTGGAACCA
  CTGAAGCGGACAGAGGAAGAGCCACTGGAAGAGCCACTGGATGAGGCCCTAGTGCGGACA
  GTCGCCCTAAGCAGTGCAGGTGTCTTGAGCCCTATGGTGGCCATGCTGGGTGCAGTAGCT
  GCCCAGGAAGTGCTGAAGGCAATCTCCAGGAAGTTCATGCCTCTGGACCAGTGGCTTTAC
  TTTGATGCCCTCGATTGTCTTCCGGAAGATGGGGAGCTCCTTCCCAGTCCTGAGGACTGT
  GCCCTGAGAGGCAGCCGCTATGATGGGCAAATTGCAGTGTTTGGGGCTGGTTTTCAGGAG
  AAACTGAGACGCCAGCACTACCTCCTGGTGGGCGCTGGTGCCATTGGTTGTGAGCTGCTC
  AAAGTCTTTGCCCTAGTGGGACTGGGGGCCGGGAACAGCGGGGGCTTGACTGTTGTTGAC
  ATGGACCACATAGAGCGCTCCAATCTCAGCCGTCAGTTCCTCTTCAGGTCCCAGGACGTT
  GGTAGACCCAAGGCAGAGGTGGCTGCAGCAGCTGCCCGGGGCCTGAACCCAGACTTACAG
  GTGATCCCGCTCACCTACCCACTGGATCCCACCACAGAGCACATCTATGGGGATAACTTT
  TTCTCCCGTGTGGATGGTGTGGCTGCTGCCCTGGACAGTTTCCAGGCCCGGCGCTATGTG
  GCTGCTCGTTGCACCCACTATCTGAAGCCACTGCTGGAGGCAGGCACATCGGGCACCTGG
  GGCAGTGCTACAGTATTCATGCCACATGTGACTGAGGCCTACAGAGCCCCTGCCTCAGCT
  GCAGCTTCTGAGGATGCCCCCTACCCTGTCTGTACCGTGCGGTACTTCCCTAGCACAGCC
  GAGCACACCCTGCAGTGGGCCCGGCATGAGTTTGAAGAACTCTTCCGACTGTCTGCAGAG
  ACCATCAACCACCACCAACAGGCACACACTTCCCTGGCAGACATGGATGAGCCACAGACA
  CTCACCTTACTGAAGCCAGTGCTTGGGGTCCTGAGAGTGCGTCCACAGAACTGGCAAGAC
  TGTGTGGCGTGGGCTCTTGGCCACTGGAAACTCTGCTTTCATTATGGCATCAAACAGCTG
  CTGAGGCACTTCCCACCTAATAAAGTGCTTGAGGATGGAACTCCCTTCTGGTCAGGTCCC
  AAACAGTGTCCCCAGCCCTTGGAGTTTGACACCAACCAAGACACACACCTCCTCTACGTA
  CTGGCAGCTGCCAACCTGTATGCCCAGATGCATGGGCTGCCTGGCTCACAGGACTGGACT
  GCACTCAGGGAGCTGCTGAAGCTGCTGCCACAGCCTGACCCCCAACAGATGGCCCCCATC
  TTTGCTAGTAATCTAGAGCTGGCTTCGGCTTCTGCTGAGTTTGGCCCTGAGCAGCAGAAG
  GAACTGAACAAAGCCCTGGAAGTCTGGAGTGTGGGCCCTCCCCTGAAGCCTCTGATGTTT
  GAGAAGGATGATGACAGCAACTTCCATGTGGACTTTGTGGTAGCGGCAGCTAGCCTGAGA
  TGTCAGAACTACGGGATTCCACCGGTCAACCGTGCCCAGAGCAAGCGAATTGTGGGCCAG
  ATTATCCCAGCCATTGCCACCACTACAGCAGCTGTGGCAGGCCTGTTGGGCCTGGAGCTG
  TATAAGGTGGTGAGTGGGCCACGGCCTCGTAGTGCCTTTCGCCACAGCTACCTACATCTG
  GCTGAAAACTACCTCATCCGCTATATGCCTTTTGCCCCAGCCATCCAGACGTTCCATCAC
  CTGAAGTGGACCTCTTGGGACCGTCTGAAGGTACCAGCTGGGCAGCCTGAGAGGACCCTG
  GAGTCGCTGCTGGCTCATCTTCAGGAGCAGCACGGGTTGAGGGTGAGGATCCTGCTGCAC
  GGCTCAGCCCTGCTCTATGCGGCCGGATGGTCACCTGAAAAGCAGGCCCAGCACCTGCCC
  CTCAGGGTGACAGAACTGGTTCAGCAGCTGACAGGCCAGGCACCTGCTCCTGGGCAGCGG
  GTGTTGGTGCTAGAGCTGAGCTGTGAGGGTGACGACGAGGACACTGCCTTCCCACCTCTG
  CACTATGAGCTGTGACAAGGCAGCCACCCTGTCACCTAGCTCAATGGAGCCCCGGATCCC
  AAGCCCTGCATTGTAAGCCCACAGTAGGCACTCAATAAATGCTTGTTAAAGGAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_003335
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003335.2, NP_003326.2
• RefSeq Size: 3330 bp
• RefSeq ORF: 3039 bp
• Locus ID: 7318
• Cytogenetics: 3p21.31
• Domains: UBACT, ThiF
• Protein Families: Druggable Genome
• Protein Pathways: Parkinson's disease, Ubiquitin mediated proteolysis
• Gene Summary: 'The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme is a retinoid target that triggers promyelocytic leukemia (PML)/retinoic acid receptor alpha (RARalpha) degradation and apoptosis in acute promyelocytic leukemia, where it is involved in the conjugation of the ubiquitin-like interferon-stimulated gene 15 protein. [provided by RefSeq, Jul 2008]'