Ube1L (UBA7) (NM_003335) Human Untagged Clone

CAT#: SC319174

UBA7 (untagged)-Human ubiquitin-like modifier activating enzyme 7 (UBA7)


  "NM_003335" in other vectors (6)

Reconstitution Protocol

USD 310.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol UBA7
Synonyms D8; UBA1B; UBE1L; UBE2
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>OriGene sequence for NM_003335.2 GGCTACAGCAGGGCACTGGCCCCTACTGTCACCGCCACCTACACAAAGACCCTATCTCTG
AGCGCTGCAGCCTACTGTTCAGCCCCAGGTTTGAGGATGGATGCCCTGGACGCTTCGAAG
CTACTGGATGAGGAGCTGTATTCAAGACAGCTGTATGTGCTGGGCTCACCTGCCATGCAG
AGGATTCAGGGAGCCAGGGTCCTGGTGTCAGGCCTGCAGGGCCTGGGGGCCGAGGTGGCC
AAGAACTTGGTTCTGATGGGTGTGGGCAGCCTCACTCTGCATGATCCCCACCCCACCTGC
TGGTCCGACCTGGCTGCCCAGTTTCTCCTCTCAGAGCAGGACTTGGAAAGGAGCAGAGCC
GAGGCCTCTCAAGAGCTCTTGGCTCAGCTCAACAGAGCTGTCCAGGTCGTCGTGCACACG
GGTGACATCACTGAGGACCTGCTGTTGGACTTCCAGGTGGTGGTGCTGACTGCTGCAAAG
CTGGAGGAGCAGCTGAAGGTGGGCACCTTGTGTCATAAGCATGGAGTTTGCTTTCTGGCG
GCTGACACCCGGGGCCTCGTGGGGCAGTTGTTCTGTGACTTTGGTGAGGACTTCACTGTG
CAGGACCCCACAGAGGCAGAACCCCTGACAGCTGCCATCCAGCACATCTCCCAGGGCTCC
CCTGGCATTCTCACTCTGAGGAAAGGGGCCAATACCCACTACTTCCGTGATGGAGACTTG
GTGACTTTCTCGGGAATTGAGGGAATGGTTGAGCTCAACGACTGTGATCCCCGGTCTATC
CACGTGCGGGAGGATGGGTCCCTGGAGATTGGAGACACAACAACTTTCTCTCGGTACTTG
CGTGGTGGGGCTATCACTGAAGTCAAGAGACCCAAGACTGTGAGACATAAGTCCCTGGAC
ACAGCCCTGCTCCAGCCCCATGTGGTGGCCCAGAGCTCCCAGGAAGTTCACCATGCCCAC
TGCCTGCATCAGGCCTTCTGTGCACTGCACAAGTTCCAGCACCTCCATGGCCGGCCACCC
CAGCCCTGGGATCCTGTTGATGCAGAGACTGTGGTGGGCCTGGCCCGGGACCTGGAACCA
CTGAAGCGGACAGAGGAAGAGCCACTGGAAGAGCCACTGGATGAGGCCCTAGTGCGGACA
GTCGCCCTAAGCAGTGCAGGTGTCTTGAGCCCTATGGTGGCCATGCTGGGTGCAGTAGCT
GCCCAGGAAGTGCTGAAGGCAATCTCCAGGAAGTTCATGCCTCTGGACCAGTGGCTTTAC
TTTGATGCCCTCGATTGTCTTCCGGAAGATGGGGAGCTCCTTCCCAGTCCTGAGGACTGT
GCCCTGAGAGGCAGCCGCTATGATGGGCAAATTGCAGTGTTTGGGGCTGGTTTTCAGGAG
AAACTGAGACGCCAGCACTACCTCCTGGTGGGCGCTGGTGCCATTGGTTGTGAGCTGCTC
AAAGTCTTTGCCCTAGTGGGACTGGGGGCCGGGAACAGCGGGGGCTTGACTGTTGTTGAC
ATGGACCACATAGAGCGCTCCAATCTCAGCCGTCAGTTCCTCTTCAGGTCCCAGGACGTT
GGTAGACCCAAGGCAGAGGTGGCTGCAGCAGCTGCCCGGGGCCTGAACCCAGACTTACAG
GTGATCCCGCTCACCTACCCACTGGATCCCACCACAGAGCACATCTATGGGGATAACTTT
TTCTCCCGTGTGGATGGTGTGGCTGCTGCCCTGGACAGTTTCCAGGCCCGGCGCTATGTG
GCTGCTCGTTGCACCCACTATCTGAAGCCACTGCTGGAGGCAGGCACATCGGGCACCTGG
GGCAGTGCTACAGTATTCATGCCACATGTGACTGAGGCCTACAGAGCCCCTGCCTCAGCT
GCAGCTTCTGAGGATGCCCCCTACCCTGTCTGTACCGTGCGGTACTTCCCTAGCACAGCC
GAGCACACCCTGCAGTGGGCCCGGCATGAGTTTGAAGAACTCTTCCGACTGTCTGCAGAG
ACCATCAACCACCACCAACAGGCACACACTTCCCTGGCAGACATGGATGAGCCACAGACA
CTCACCTTACTGAAGCCAGTGCTTGGGGTCCTGAGAGTGCGTCCACAGAACTGGCAAGAC
TGTGTGGCGTGGGCTCTTGGCCACTGGAAACTCTGCTTTCATTATGGCATCAAACAGCTG
CTGAGGCACTTCCCACCTAATAAAGTGCTTGAGGATGGAACTCCCTTCTGGTCAGGTCCC
AAACAGTGTCCCCAGCCCTTGGAGTTTGACACCAACCAAGACACACACCTCCTCTACGTA
CTGGCAGCTGCCAACCTGTATGCCCAGATGCATGGGCTGCCTGGCTCACAGGACTGGACT
GCACTCAGGGAGCTGCTGAAGCTGCTGCCACAGCCTGACCCCCAACAGATGGCCCCCATC
TTTGCTAGTAATCTAGAGCTGGCTTCGGCTTCTGCTGAGTTTGGCCCTGAGCAGCAGAAG
GAACTGAACAAAGCCCTGGAAGTCTGGAGTGTGGGCCCTCCCCTGAAGCCTCTGATGTTT
GAGAAGGATGATGACAGCAACTTCCATGTGGACTTTGTGGTAGCGGCAGCTAGCCTGAGA
TGTCAGAACTACGGGATTCCACCGGTCAACCGTGCCCAGAGCAAGCGAATTGTGGGCCAG
ATTATCCCAGCCATTGCCACCACTACAGCAGCTGTGGCAGGCCTGTTGGGCCTGGAGCTG
TATAAGGTGGTGAGTGGGCCACGGCCTCGTAGTGCCTTTCGCCACAGCTACCTACATCTG
GCTGAAAACTACCTCATCCGCTATATGCCTTTTGCCCCAGCCATCCAGACGTTCCATCAC
CTGAAGTGGACCTCTTGGGACCGTCTGAAGGTACCAGCTGGGCAGCCTGAGAGGACCCTG
GAGTCGCTGCTGGCTCATCTTCAGGAGCAGCACGGGTTGAGGGTGAGGATCCTGCTGCAC
GGCTCAGCCCTGCTCTATGCGGCCGGATGGTCACCTGAAAAGCAGGCCCAGCACCTGCCC
CTCAGGGTGACAGAACTGGTTCAGCAGCTGACAGGCCAGGCACCTGCTCCTGGGCAGCGG
GTGTTGGTGCTAGAGCTGAGCTGTGAGGGTGACGACGAGGACACTGCCTTCCCACCTCTG
CACTATGAGCTGTGACAAGGCAGCCACCCTGTCACCTAGCTCAATGGAGCCCCGGATCCC
AAGCCCTGCATTGTAAGCCCACAGTAGGCACTCAATAAATGCTTGTTAAAGGAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites Please inquire     
ACCN NM_003335
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003335.2, NP_003326.2
RefSeq Size 3330 bp
RefSeq ORF 3039 bp
Locus ID 7318
Cytogenetics 3p21.31
Domains UBACT, ThiF
Protein Families Druggable Genome
Protein Pathways Parkinson's disease, Ubiquitin mediated proteolysis
Gene Summary 'The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme is a retinoid target that triggers promyelocytic leukemia (PML)/retinoic acid receptor alpha (RARalpha) degradation and apoptosis in acute promyelocytic leukemia, where it is involved in the conjugation of the ubiquitin-like interferon-stimulated gene 15 protein. [provided by RefSeq, Jul 2008]'

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