PUF60 (NM_014281) Human Untagged Clone

CAT#: SC319186

PUF60 (untagged)-Human poly-U binding splicing factor 60KDa (PUF60), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PUF60
• Synonyms: FIR; RoBPI; SIAHBP1; VRJS
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_014281.3 GGAAGGAGCAAGAGTGGGAGGCGCGCGCGGAGGCCGCGACGGACGCAAGATGGCGACGGC
  GACCATAGCTCTCCAGGTCAATGGCCAGCAAGGAGGGGGGTCCGAGCCGGCGGCGGCGGC
  GGCAGTGGTGGCAGCGGGAGACAAATGGAAACCTCCACAGGGCACAGACTCCATCAAGAT
  GGAGAACGGGCAGAGCACAGCCGCCAAGCTGGGGCTGCCTCCCCTGACGCCCGAGCAGCA
  GGAGGCCCTTCAGAAGGCCAAGAAGTACGCCATGGAGCAGAGCATCAAGAGTGTGCTGGT
  GAAGCAGACCATCGCGCACCAGCAGCAGCAGCTCACCAACCTGCAGATGGCGGCTCAGCG
  GCAGCGGGCGCTGGCCATCATGTGCCGCGTCTACGTGGGCTCTATCTACTATGAGCTGGG
  GGAGGACACCATCCGCCAGGCCTTTGCCCCCTTTGGCCCCATCAAGAGCATCGACATGTC
  CTGGGACTCCGTCACCATGAAGCACAAGGGCTTTGCCTTCGTGGAGTATGAGGTCCCCGA
  AGCTGCACAGCTGGCCTTGGAGCAGATGAACTCGGTGATGCTGGGGGGCAGGAACATCAA
  GGTGGGCAGACCCAGCAACATAGGGCAGGCCCAGCCCATCATAGACCAGTTGGCTGAGGA
  GGCACGGGCCTTCAACCGCATCTACGTGGCCTCTGTGCACCAGGACCTCTCAGACGATGA
  CATCAAGAGCGTGTTTGAGGCCTTTGGCAAGATCAAGTCCTGCACACTGGCCCGGGACCC
  CACAACTGGCAAGCACAAGGGCTACGGCTTCATTGAGTACGAGAAGGCCCAGTCGTCCCA
  AGATGCTGTGTCTTCCATGAACCTCTTTGACCTGGGTGGCCAGTACTTGCGGGTGGGCAA
  GGCTGTCACACCGCCCATGCCCCTACTCACACCAGCCACGCCTGGAGGCCTCCCACCTGC
  CGCTGCTGTGGCAGCTGCTGCAGCCACTGCCAAGATCACAGCTCAGGAAGCAGTGGCCGG
  AGCAGCGGTGCTGGGTACCCTGGGCACACCTGGACTGGTGTCCCCAGCACTGACCCTGGC
  CCAGCCCCTGGGCACTTTGCCCCAGGCTGTCATGGCTGCCCAGGCACCTGGAGTCATCAC
  AGGTGTGACCCCAGCCCGTCCTCCTATCCCGGTCACCATCCCCTCGGTGGGAGTGGTGAA
  CCCCATCCTGGCCAGCCCTCCAACGCTGGGTCTCCTGGAGCCCAAGAAGGAGAAGGAAGA
  AGAGGAGCTGTTTCCCGAGTCAGAGCGGCCAGAGATGCTGAGCGAGCAGGAGCACATGAG
  CATCTCGGGCAGTAGCGCCCGACACATGGTGATGCAGAAGCTGCTCCGCAAGCAGGAGTC
  TACAGTGATGGTTCTGCGCAACATGGTGGACCCCAAGGACATCGATGATGACCTGGAAGG
  GGAGGTGACAGAGGAGTGTGGCAAGTTCGGGGCCGTGAACCGCGTCATCATCTACCAAGA
  GAAACAAGGCGAGGAGGAGGATGCAGAAATCATTGTCAAGATCTTTGTGGAGTTTTCCAT
  AGCCTCTGAGACTCATAAGGCCATCCAGGCCCTCAATGGCCGCTGGTTTGCTGGCCGCAA
  GGTGGTGGCTGAAGTGTACGACCAGGAGCGTTTTGATAACAGTGACCTCTCTGCGTGACA
  GTGGTCCCTCTCCCCGGACTTGCACTTGTTCCTTGTTTCCTCTGGGTTTTATAGTGATAC
  AGTGGTGTCCCCGGGGCCAGGCGCGCTCTGCCCAGCCCAGCCTACAGTGCGGATAAAGGT
  GCGGATGCTGCTGGCCCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAA
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014281.3 unedited
  TTCACGTGTATACGACTCCTATAGGGCGGCCGGGTAATTCGGCACGAGGGGAAGGAGCAA
  GAGTGGGAGGCGCGCGCGGAGGCCGCGACGGACGCAAGATGGCGACGGCGACCATAGCTC
  TCCAGGTCAATGGCCAGCAAGGAGGGGGGTCCGAGCCGGCGGCGGCGGCGGCAGTGGTGG
  CAGCGGGAGACAAATGGAAACCTCCACAGGGCACAGACTCCATCAAGATGGAGAACGGGC
  AGAGCACAGCCGCCAAGCTGGGGCTGCCTCCCCTGACGCCCGAGCAGCAGGAGGCCCTTC
  AGAAGGCCAAGAAGTACGCCATGGAGCAGAGCATCAAGAGTGTGCTGGTGAAGCAGACCA
  TCGCGCACCAGCAGCAGCAGCTCACCAACCTGCAGATGGCGGCTCAGCGGCAGCGGGCGC
  TGGCCATCATGTGCCGCGTCTACGTGGGCTCTATCTACTATGAGCTGGGGGAGGACACCA
  TCCGCCAGGCCTTTGCCCCCTTTGGCCCCATCAAGAGCATCGACATGTCCTGGGACTCCG
  TCACCATGAAGCACAAGGGCTTTGCCTTCGTGGAGTATGAGGTCCCCGAAGCTGCACAGC
  TGGCCTTGGAGCAGATGAACTCGGTGATGCTGGGGGGCAGGAACATCAATGTGGGCAGAC
  CCAGCAACATAGGGCACGCCCAGCCCATCATAGACCAGTTGGCTGAGAGGCACGGGCCCT
  TCAACCGCATCTACGTGGCCTCTGTGCACCAGGACCTCTCAGACGATGACATCAAGAGCG
  TGTTTGAGCCCTTTGGCAAGATCAAGTCCTGCACACTGCCCGGGACCCCACAACTGGCAA
  GCACAAGGCTACGCTCATGAGTACGAGAAGCCAGTCGTCCAGATGCTGTGTCTTCATGAC
  TCTTTGACTTGGTGGCAGTACTGCGGTTGCAGCTGTCAACCGCCATGCCTACTCACACAA
  GACACGCCCTTGA
• Restriction Sites: Please inquire
• ACCN: NM_014281
• ORF Size: 1629 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_014281.3, NP_055096.2
• RefSeq Size: 1853
• RefSeq ORF: 1629
• Locus ID: 22827
• Domains: RRM, RRM_1
• Protein Pathways: Spliceosome
• Gene Summary: This gene encodes a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation. The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding protein at the myelocytomatosis oncogene (MYC) promoter. This complex represses MYC transcription through the core-TFIIH basal transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the coding region, compared to variant 1. The encoded isoform (b) is shorter than isoform a.