SSX2 (NM_175698) Human Untagged Clone

CAT#: SC319207

SSX2 (untagged)-Human synovial sarcoma, X breakpoint 2 (SSX2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SSX2
• Synonyms: CT5.2; CT5.2A; HD21; HOM-MEL-40; SSX
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_175698.1 CTCAGAGTACGCACGGTCTGATTTTCTCTTTGGATTCTTCCAAAATCAGAGTCAGACTGC
  TCCCGGTGCCATGAACGGAGACGACGCCTTTGCAAGGAGACCCACGGTTGGTGCTCAAAT
  ACCAGAGAAGATCCAAAAGGCCTTCGATGATATTGCCAAATACTTCTCTAAGGAAGAGTG
  GGAAAAGATGAAAGCCTCAGAGAAAATCTTCTATGTGTATATGAAGAGAAAGTATGAGGC
  TATGACTAAACTAGGTTTCAAGGCCACCCTCCCACCTTTCATGTGTAATAAACGGGCCGA
  AGACTTCCAGGGGAATGATTTGGATAATGACCCTAACCGTGGGAATCAGGTTGAACGTCC
  TCAGATGACTTTCGGCAGGCTCCAGGGAATCTCCCCGAAGATCATGCCCAAGAAGCCAGC
  AGAGGAAGGAAATGATTCGGAGGAAGTGCCAGAAGCATCTGGCCCACAAAATGATGGGAA
  AGAGCTGTGCCCCCCGGGAAAACCAACTACCTCTGAGAAGATTCACGAGAGATCTGGACC
  CAAAAGGGGGGAACATGCCTGGACCCACAGACTGCCTGAGAGAAAACAGCTGGTGATTTA
  TGAAGAGATCAGCGACCCTGAGGAAGATGACGAGTAACTCCCCTCAGGGATACGACACAT
  GCCCATGATGAGAAGCAGAACGTGGTGACCTTTCACGAACATGGGCATGGCTGCGGACCC
  CTCGTCATCAGGTGCATAGCAAGTGAAAGCAAGTGTTCACAACAGTGAAAAGTTGAGCGT
  CATTTTTCTTAGTGTGCCAAGAGTTCGATGTTAGCGTTTACGTTGTATTTTCTTACACTG
  TGTCATTCTGTTAGATACTAACATTTTCATTGATGAGCAAGACATACTTAATGCATATTT
  TGGTTTGTGTATCCATGCACCTACCTTAGAAAACAAGTATTGTCGGTTACCTCTGCATGG
  AACAGCATTACCCTCCTCTCTCCCCAGATGTGACTACTGAGGGCAGTTCTGAGTGTTTAA
  TTTCAGATTTTTTCCTCTGCATTTACACACACACGCACACAAACCACACCACACACACAC
  ACACACACACACACACACACACACACACACCAAGTACCAGTATAAGCATCTGCCATCTGC
  TTTTCCCATTGCCATGCGTCCTGGTCAAGCTCCCCTCACTCTGTTTCCTGGTCAGCATGT
  ACTCCCCTCATCCGATTCCCCTGTAGCAGTCACTGACAGTTAATAAACCTTTGCAAACGT
  TCAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_175698
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175698.1, NP_783629.1
• RefSeq Size: 1322 bp
• RefSeq ORF: 567 bp
• Locus ID: 6757
• Cytogenetics: Xp11.22
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: 'The product of this gene belongs to the family of highly homologous synovial sarcoma X (SSX) breakpoint proteins. These proteins may function as transcriptional repressors. They are also capable of eliciting spontaneous humoral and cellular immune responses in cancer patients, and are potentially useful targets in cancer vaccine-based immunotherapy. This gene, and also the SSX1 and SSX4 family members, have been involved in t(X;18)(p11.2;q11.2) translocations that are characteristically found in all synovial sarcomas. This translocation results in the fusion of the synovial sarcoma translocation gene on chromosome 18 to one of the SSX genes on chromosome X. The encoded hybrid proteins are likely responsible for transforming activity. Alternative splicing of this gene results in multiple transcript variants. This gene also has an identical duplicate, GeneID: 727837, located about 45 kb downstream in the opposite orientation on chromosome X. [provided by RefSeq, Jul 2013]'
  Transcript Variant: This variant (2) lacks an alternate exon which results in a frameshift in the 3' coding region, compared to variant 1. The encoded isoform (b) contains a shorter and distinct C-terminus, compared to isoform a.