Methionyl tRNA synthetase (MARS) (NM_004990) Human Untagged Clone

CAT#: SC319245

MARS (untagged)-Human methionyl-tRNA synthetase (MARS)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MARS
• Synonyms: CMT2U; ILFS2; ILLD; METRS; MRS; MTRNS; SPG70
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004990.2 ACGGCGAAATGAGACTGTTCGTGAGTGATGGCGTCCCGGGTTGCTTGCCGGTGCTGGCCG
  CCGCCGGGAGAGCCCGGGGCAGAGCAGAGGTGCTCATCAGCACTGTAGGCCCGGAAGATT
  GTGTGGTCCCGTTCCTGACCCGGCCTAAGGTCCCTGTCTTGCAGCTGGATAGCGGCAACT
  ACCTCTTCTCCACTAGTGCAATCTGCCGATATTTTTTTTTGTTATCTGGCTGGGAGCAAG
  ATGACCTCACTAACCAGTGGCTGGAATGGGAAGCGACAGAGCTGCAGCCAGCTTTGTCTG
  CTGCCCTGTACTATTTAGTGGTCCAAGGCAAGAAGGGGGAAGATGTTCTTGGTTCAGTGC
  GGAGAGCCCTGACTCACATTGACCACAGCTTGAGTCGTCAGAACTGTCCTTTCCTGGCTG
  GGGAGACAGAATCTCTAGCCGACATTGTTTTGTGGGGAGCCCTATACCCATTACTGCAAG
  ATCCCGCCTACCTCCCTGAGGAGCTGAGTGCCCTGCACAGCTGGTTCCAGACACTGAGTA
  CCCAGGAACCATGTCAGCGAGCTGCAGAGACTGTACTGAAACAGCAAGGTGTCCTGGCTC
  TCCGGCCTTACCTCCAAAAGCAGCCCCAGCCCAGCCCCGCTGAGGGAAGGGCTGTCACCA
  ATGAGCCTGAGGAGGAGGAGCTGGCTACCCTATCTGAGGAGGAGATTGCTATGGCTGTTA
  CTGCTTGGGAGAAGGGCCTAGAAAGTTTGCCCCCGCTGCGGCCCCAGCAGAATCCAGTGT
  TGCCTGTGGCTGGAGAAAGGAATGTGCTCATCACCAGTGCCCTCCCTTACGTCAACAATG
  TCCCCCACCTTGGGAACATCATTGGTTGTGTGCTCAGTGCCGATGTCTTTGCCAGGTACT
  CTCGCCTCCGCCAGTGGAACACCCTCTATCTGTGTGGGACAGATGAGTATGGTACAGCAA
  CAGAGACCAAGGCTCTGGAGGAGGGACTAACCCCCCAGGAGATCTGCGACAAGTACCACA
  TCATCCATGCTGACATCTACCGCTGGTTTAACATTTCGTTTGATATTTTTGGTCGCACCA
  CCACTCCACAGCAGACCAAAATCACCCAGGACATTTTCCAGCAGTTGCTGAAACGAGGTT
  TTGTGCTGCAAGATACTGTGGAGCAACTGCGATGTGAGCACTGTGCTCGCTTCCTGGCTG
  ACCGCTTCGTGGAGGGCGTGTGTCCCTTCTGTGGCTATGAGGAGGCTCGGGGTGACCAGT
  GTGACAAGTGTGGCAAGCTCATCAATGCTGTCGAGCTTAAGAAGCCTCAGTGTAAAGTCT
  GCCGATCATGCCCTGTGGTGCAGTCGAGCCAGCACCTGTTTCTGGACCTGCCTAAGCTGG
  AGAAGCGACTGGAGGAGTGGTTGGGGAGGACATTGCCTGGCAGTGACTGGACACCCAATG
  CCCAGTTTATCACCCGTTCTTGGCTTCGGGATGGCCTCAAGCCACGCTGCATAACCCGAG
  ACCTCAAATGGGGAACCCCTGTACCCTTAGAAGGTTTTGAAGACAAGGTATTCTATGTCT
  GGTTTGATGCCACTATTGGCTATCTGTCCATCACAGCCAACTACACAGACCAGTGGGAGA
  GATGGTGGAAGAACCCAGAGCAAGTGGACCTGTATCAGTTCATGGCCAAAGACAATGTTC
  CTTTCCATAGCTTAGTCTTTCCTTGCTCAGCCCTAGGAGCTGAGGATAACTATACCTTGG
  TCAGCCACCTCATTGCTACAGAGTACCTGAACTATGAGGATGGGAAATTCTCTAAGAGCC
  GCGGTGTGGGAGTGTTTGGGGACATGGCCCAGGACACGGGGATCCCTGCTGACATCTGGC
  GCTTCTATCTGCTGTACATTCGGCCTGAGGGCCAGGACAGTGCTTTCTCCTGGACGGACC
  TGCTGCTGAAGAATAATTCTGAGCTGCTTAACAACCTGGGCAACTTCATCAACAGAGCTG
  GGATGTTTGTGTCTAAGTTCTTTGGGGGCTATGTGCCTGAGATGGTGCTCACCCCTGATG
  ATCAGCGCCTGCTGGCCCATGTCACCCTGGAGCTCCAGCACTATCACCAGCTACTTGAGA
  AGGTTCGGATCCGGGATGCCTTGCGCAGTATCCTCACCATATCTCGACATGGCAACCAAT
  ATATTCAGGTGAATGAGCCCTGGAAGCGGATTAAAGGCAGTGAGGCTGACAGGCAACGGG
  CAGGAACAGTGACTGGCTTGGCAGTGAATATAGCTGCCTTGCTCTCTGTCATGCTTCAGC
  CTTACATGCCCACGGTTAGTGCCACAATCCAGGCCCAGCTGCAGCTCCCACCTCCAGCCT
  GCAGTATCCTGCTGACAAACTTCCTGTGTACCTTACCAGCAGGACACCAGATTGGCACAG
  TCAGTCCCTTGTTCCAAAAATTGGAAAATGACCAGATTGAAAGTTTAAGGCAGCGCTTTG
  GAGGGGGCCAGGCAAAAACGTCCCCGAAGCCAGCAGTTGTAGAGACTGTTACAACAGCCA
  AGCCACAGCAGATACAAGCGCTGATGGATGAAGTGACAAAACAAGGAAACATTGTCCGAG
  AACTGAAAGCACAAAAGGCAGACAAGAACGAGGTTGCTGCGGAGGTGGCGAAACTCTTGG
  ATCTAAAGAAACAGTTGGCTGTAGCTGAGGGGAAACCCCCTGAAGCCCCTAAAGGCAAGA
  AGAAAAAGTAAAAGACCTTGGCTCATAGAAAGTCACTTTAATAGATAGGGACAGTAATAA
  ATAAATGTACAATCTCTATAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_004990
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004990.2, NP_004981.2
• RefSeq Size: 2795 bp
• RefSeq ORF: 2703 bp
• Locus ID: 4141
• Cytogenetics: 12q13.3
• Domains: WHEP-TRS, tRNA-synt_1, GST_C
• Protein Families: Druggable Genome
• Protein Pathways: Aminoacyl-tRNA biosynthesis, Selenoamino acid metabolism
• Gene Summary: 'This gene encodes a member of the class I family of aminoacyl-tRNA synthetases. These enzymes play a critical role in protein biosynthesis by charging tRNAs with their cognate amino acids. The encoded protein is a component of the multi-tRNA synthetase complex and catalyzes the ligation of methionine to tRNA molecules. [provided by RefSeq, Jan 2011]'