MCM2 (NM_004526) Human Untagged Clone

CAT#: SC319263

MCM2 (untagged)-Human minichromosome maintenance complex component 2 (MCM2)


  "NM_004526" in other vectors (6)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol MCM2
Synonyms BM28; CCNL1; cdc19; CDCL1; D3S3194; DFNA70; MITOTIN
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>OriGene sequence for NM_004526.2 CGCGAAACCTGGTTGTTGCTGTAGTGGCGGAGAGGATCGTGGTACTGCTATGGCGGAATC
ATCGGAATCCTTCACCATGGCATCCAGCCCGGCCCAGCGTCGGCGAGGCAATGATCCTCT
CACCTCCAGCCCTGGCCGAAGCTCCCGGCGTACTGATGCCCTCACCTCCAGCCCTGGCCG
TGACCTTCCACCATTTGAGGATGAGTCCGAGGGGCTCCTAGGCACAGAGGGGCCCCTGGA
GGAAGAAGAGGATGGAGAGGAGCTCATTGGAGATGGCATGGAAAGGGACTACCGCGCCAT
CCCAGAGCTGGACGCCTATGAGGCCGAGGGACTGGCTCTGGATGATGAGGACGTAGAGGA
GCTGACGGCCAGTCAGAGGGAGGCAGCAGAGCGGGCCATGCGGCAGCGTGACCGGGAGGC
TGGCCGGGGCCTGGGCCGCATGCGCCGTGGGCTCCTGTATGACAGCGATGAGGAGGACGA
GGAGCGCCCTGCCCGCAAGCGCCGCCAGGTGGAGCGGGCCACGGAGGACGGCGAGGAGGA
CGAGGAGATGATTGAGAGCATCGAGAACCTGGAGGATCTCAAAGGCCACTCTGTGCGCGA
GTGGGTGAGCATGGCGGGCCCCCGGCTGGAGATCCACCACCGCTTCAAGAACTTCCTGCG
CACTCACGTCGACAGCCACGGCCACAACGTCTTCAAGGAGCGCATCAGCGACATGTGCAA
AGAGAACCGTGAGAGCCTGGTGGTGAACTATGAGGACTTGGCAGCCAGGGAGCACGTGCT
GGCCTACTTCCTGCCTGAGGCACCGGCGGAGCTGCTGCAGATCTTTGATGAGGCTGCCCT
GGAGGTGGTACTGGCCATGTACCCCAAGTACGACCGCATCACCAACCACATCCATGTCCG
CATCTCCCACCTGCCTCTGGTGGAGGAGCTGCGCTCGCTGAGGCAGCTGCATCTGAACCA
GCTGATCCGCACCAGTGGGGTGGTGACCAGCTGCACTGGCGTCCTGCCCCAGCTCAGCAT
GGTCAAGTACAACTGCAACAAGTGCAATTTCGTCCTGGGTCCTTTCTGCCAGTCCCAGAA
CCAGGAGGTGAAACCAGGCTCCTGTCCTGAGTGCCAGTCGGCCGGCCCCTTTGAGGTCAA
CATGGAGGAGACCATCTATCAGAACTACCAGCGTATCCGAATCCAGGAGAGTCCAGGCAA
AGTGGCGGCTGGCCGGCTGCCCCGCTCCAAGGACGCCATTCTCCTCGCAGATCTGGTGGA
CAGCTGCAAGCCAGGAGACGAGATAGAGCTGACTGGCATCTATCACAACAACTATGATGG
CTCCCTCAACACTGCCAATGGCTTCCCTGTCTTTGCCACTGTCATCCTAGCCAACCACGT
GGCCAAGAAGGACAACAAGGTTGCTGTAGGGGAACTGACCGATGAAGATGTGAAGATGAT
CACTAGCCTCTCCAAGGATCAGCAGATCGGAGAGAAGATCTTTGCCAGCATTGCTCCTTC
CATCTATGGTCATGAAGACATCAAGAGAGGCCTGGCTCTGGCCCTGTTCGGAGGGGAGCC
CAAAAACCCAGGTGGCAAGCACAAGGTACGTGGTGATATCAACGTGCTCTTGTGCGGAGA
CCCTGGCACAGCGAAGTCGCAGTTTCTCAAGTATATTGAGAAAGTGTCCAGCCGAGCCAT
CTTCACCACTGGCCAGGGGGCGTCGGCTGTGGGCCTCACGGCGTATGTCCAGCGGCACCC
TGTCAGCAGGGAGTGGACCTTGGAGGCTGGGGCCCTGGTTCTGGCTGACCGAGGAGTGTG
TCTCATTGATGAATTTGACAAGATGAATGACCAGGACAGAACCAGCATCCATGAGGCCAT
GGAGCAACAGAGCATCTCCATCTCGAAGGCTGGCATCGTCACCTCCCTGCAGGCTCGCTG
CACGGTCATTGCTGCCGCCAACCCCATAGGAGGGCGCTACGACCCCTCGCTGACTTTCTC
TGAGAACGTGGACCTCACAGAGCCCATCATCTCACGCTTTGACATCCTGTGTGTGGTGAG
GGACACCGTGGACCCAGTCCAGGACGAGATGCTGGCCCGCTTCGTGGTGGGCAGCCACGT
CAGACACCACCCCAGCAACAAGGAGGAGGAGGGGCTGGCCAATGGCAGCGCTGCTGAGCC
CGCCATGCCCAACACGTATGGCGTGGAGCCCCTGCCCCAGGAGGTCCTGAAGAAGTACAT
CATCTACGCCAAGGAGAGGGTCCACCCGAAGCTCAACCAGATGGACCAGGACAAGGTGGC
CAAGATGTACAGTGACCTGAGGAAAGAATCTATGGCGACAGGCAGCATCCCCATTACGGT
GCGGCACATCGAGTCCATGATCCGCATGGCGGAGGCCCACGCGCGCATCCATCTGCGGGA
CTATGTGATCGAAGACGACGTCAACATGGCCATCCGCGTGATGCTGGAGAGCTTCATAGA
CACACAGAAGTTCAGCGTCATGCGCAGCATGCGCAAGACTTTTGCCCGCTACCTTTCATT
CCGGCGTGACAACAATGAGCTGTTGCTCTTCATACTGAAGCAGTTAGTGGCAGAGCAGGT
GACATATCAGCGCAACCGCTTTGGGGCCCAGCAGGACACTATTGAGGTCCCTGAGAAGGA
CTTGGTGGATAAGGCTCGTCAGATCAACATCCACAACCTCTCTGCATTTTATGACAGTGA
GCTCTTCAGGATGAACAAGTTCAGCCACGACCTGAAAAGGAAAATGATCCTGCAGCAGTT
CTGAGGCCCTATGCCATCCATAAGGATTCCTTGGGATTCTGGTTTGGGGTGGTCAGTGCC
CTCTGTGCTTTATGGACACAAAACCAGAGCACTTGATGAACTCGGGGTACTAGGGTCAGG
GCTTATAGCAGGATGTCTGGCTGCACCTGGCATGACTGTTTGTTTCTCCAAGCCTGCTTT
GTGCTTCTCACCTTTGGGTGGGATGCCTTGCCAGTGTGTCTTACTTGGTTGCTGAACATC
TTGCCACCTCCGAGTGCTTTGTCTCCACTCAGTACCTTGGATCAGAGCTGCTGAGTTCAG
GATGCCTGCGTGTGGTTTAGGTGTTAGCCTTCTTACATGGATGTCAGGAGAGCTGCTGCC
CTCTTGGCGTGAGTTGCGTATTCAGGCTGCTTTTGCTGCCTTTGGCCAGAGAGCTGGTTG
AAGATGTTTGTAATCGTTTTCAGTCTCCTGCAGGTTTCTGTGCCCCTGTGGTGGAAGAGG
GCACGACAGTGCCAGCGCAGCGTTCTGGGCTCCTCAGTCGCAGGGGTGGGATGTGAGTCA
TGCGGATTATCCACTCGCCACAGTTATCAGCTGCCATTGCTCCCTGTCTGTTTCCCCACT
CTCTTATTTGTGCATTCGGTTTGGTTTCTGTAGTTTTAATTTTTAATAAAGTTGAATAAA
ATATAAAAAAAAAAAAAAAAAAAAA
Restriction Sites Please inquire     
ACCN NM_004526
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_004526.2, NP_004517.2
RefSeq Size 3453 bp
RefSeq ORF 2715 bp
Locus ID 4171
Cytogenetics 3q21.3
Domains MCM
Protein Families Druggable Genome, Stem cell - Pluripotency, Transcription Factors
Protein Pathways Cell cycle, DNA replication
Gene Summary 'The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Oct 2012]'
Transcript Variant: This variant (1) encodes a functional protein.

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