MRPL4 (NM_146387) Human Untagged Clone

CAT#: SC319301

MRPL4 (untagged)-Human mitochondrial ribosomal protein L4 (MRPL4), nuclear gene encoding mitochondrial protein, transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MRPL4
• Synonyms: CGI-28; L4mt
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_146387.1 GACCTCCCGCGGCGTGGGAGGCTGCGCGGCGATGCTGCAGTTCGTCCGGGCCGGGGCGCG
  GGCCTGGCTTCGGCCTACCGGCAGCCAGGGCCTGAGTTCCCTGGCGGAAGAGGCAGCGCG
  TGCGACCGAGAACCCGGAGCAGGTGGCGAGCGAGGGTCTCCCGGAGCCCGTGCTGCGCAA
  AGTCGAGCTCCCGGTACCCACTCATCGACGCCCAGTGCAGGCCTGGGTCGAGTCCTTGCG
  GGGCTTCGAGCAGGAGCGCGTGGGCCTGGCCGACCTGCACCCCGATGTTTTCGCCACCGC
  GCCCAGGCTGGACATACTGCACCAGGTTGCTATGTGGCAGAAGAACTTCAAGAGAATTAG
  CTATGCCAAGACCAAGACGAGAGCCGAGGTGCGGGGCGGTGGCCGGAAGCCTTGGCCGCA
  GAAAGGCACTGGGCGGGCCCGGCATGGCAGCATCCGCTCTCCGCTCTGGCGAGGAGGAGG
  TGTTGCCCATGGCCCCCGGGGCCCCACAAGTTACTACTACATGCTGCCCATGAAGGTGCG
  GGCGCTGGGTCTCAAAGTGGCACTGACCGTCAAGCTGGCCCAGGACGACCTGCACATCAT
  GGACTCCCTAGAGCTGCCCACCGGAGACCCACAGTACCTGACAGAGCTGGCGCACTACCG
  CCGCTGGGGGGACTCCGTACTCCTCGTGGACTTAACACACGAGGAGATGCCACAGAGCAT
  CGTGGAGGCCACCTCTAGGCTTAAGACCTTCAACTTGATCCCGGCTGTTGGCCTAAATGT
  GCACAGCATGCTCAAGCACCAGACGCTGGTCCTGACGCTGCCCACCGTCGCCTTCCTGGA
  GGACAAGCTGCTCTGGCAGGACTCACGTTACAGACCCCTCTACCCCTTCAGCCTGCCCTA
  CAGCGACTTCCCCCGACCCCTACCCCACGCTACCCAGGGCCCAGCGGCCACCCCGTACCA
  CTGTTGATGTGAAGCACCTCTTCTGAGCCAGGCCGAGCCCCTGGCCGACTTGGGAGCCTC
  AGGCCCACGCCCACCCTTCGAGGAAGGTGTCACCTGGACCCCTTCATTCCACGGAGGAAG
  CTGAGGCCACAGGGAGCGGCCATCGCCATTGGGAAGGGGCGACTCCACGGAAAGCCCAGA
  CGGGCTTCTGCATCCATTCCCTCTTTTTGTTTTTAAAATAAATTGTATTTTTGAATCAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_146387.1 unedited
  GACACCTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGGCACGAGGGACCTCCCGCG
  GCGTGGGAGGCTGCGCGGCGATGCTGCAGTTCGTCCGGGCCGGGGCGCGGGCCTGGCTTC
  GGCCTACCGGCAGCCAGGGCCTGAGTTCCCTGGCGGAAGAGGCAGCGCGTGCGACCGAGA
  ACCCGGAGCAGGTGGCGAGCGAGGGTCTCCCGGAGCCCGTGCTGCGCAAAGTCGAGCTCC
  CGGTACCCACTCATCGACGCCCAGTGCAGGCCTGGGTCGAGTCCTTGCGGGGCTTCGAGC
  AGGAGCGCGTGGGCCTGGCCGACCTGCACCCCGATGTTTTCGCCACCGCGCCCAGGCTGG
  ACATACTGCACCAGGTTGCTATGTGGCAGAAGAACTTCAAGAGAATTAGCTATGCCAAGA
  CCAAGACGAGAGCCGAGGTGCGGGGCGGTGGCCGGAAGCCTTGGCCGCAGAAAGGCACTG
  GGCGGGCCCGGCATGGCAGCATCCGCTCTCCGCTCTGGCGAGGAGGAGGTGTTGCCCATG
  GCCCCCGGGGCCCCACAAGTTACTACTACATGCTGCCCCATGAAGGTGCGGGCGCTGGGT
  CTCAAAGTGGCACTGACCGTCAAGCTGGCCCAGGACGACCTGCACATCATGGACTCCCTA
  GAGCTGCCCACCGGAGACCCACAGTACCTGACAGAGCTGGCGCACTACCGCCGCTGGGGG
  GACTCCGTACTCCTCGTGGACTTAACACACGAGAGATGCCACAGAGCATCGTGGAGGCCA
  CCTCTAGCTTAGACCTTCAACTTGATCCCGGCTGTTGCCCTAAATGTGCACAGCATGCTC
  AAGCACCAGACGCTGGTCCTGACGCTGCCCACCGTCGCCTCTGGAGGACAAGGCTGCTCT
  GCAGACCTCACGTTACAGACCCTCTACCCTTTCAGCCTGCCTACAAGGCGAACTTTC
• Restriction Sites: Please inquire
• ACCN: NM_146387
• ORF Size: 936 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_146387.1, NP_666499.1
• RefSeq Size: 1375
• RefSeq ORF: 936
• Locus ID: 51073
• Gene Summary: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Sequence analysis identified alternatively spliced variants that encode different protein isoforms. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same isoform (a).