PFKL (NM_002626) Human Untagged Clone

CAT#: SC319353

PFKL (untagged)-Human phosphofructokinase, liver (PFKL), transcript variant 2


  "NM_002626" in other vectors (6)

Reconstitution Protocol

USD 540.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol PFKL
Synonyms ATP-PFK; PFK-B; PFK-L
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>OriGene sequence for NM_002626.4 CCCGTGTTTCGGCCGCCGCCATGGCCGCGGTGGACCTGGAGAAGCTGCGGGCGTCGGGCG
CGGGCAAGGCCATCGGCGTCCTGACCAGCGGCGGCGACGCGCAAGGCATGAACGCTGCTG
TCCGGGCTGTGACGCGCATGGGCATTTATGTGGGTGCCAAAGTCTTCCTCATCTACGAGG
GCTATGAGGGCCTCGTGGAGGGAGGTGAGAACATCAAGCAGGCCAACTGGCTGAGCGTCT
CCAACATCATCCAGCTGGGCGGCACTATCATTGGCAGCGCTCGCTGCAAGGCCTTTACCA
CCAGGGAGGGGCGCCGGGCAGCGGCCTACAACCTGGTCCAGCACGGCATCACCAACCTGT
GCGTCATCGGCGGGGATGGCAGCCTTACAGGTGCCAACATCTTCCGCAGCGAGTGGGGCA
GCCTGCTGGAGGAGCTGGTGGCGGAAGGTAAGATCTCAGAGACTACAGCCCGGACCTACT
CGCACCTGAACATCGCGGGCCTAGTGGGCTCCATCGATAACGACTTCTGCGGCACCGACA
TGACCATCGGCACGGACTCGGCCCTCCACCGCATCATGGAGGTCATCGATGCCATCACCA
CCACTGCCCAGAGCCACCAGAGGACCTTCGTGCTGGAAGTGATGGGCCGGCACTGCGGGT
ACCTGGCGCTGGTATCTGCACTGGCCTCAGGGGCCGACTGGCTGTTCATCCCCGAGGCTC
CACCCGAGGACGGCTGGGAGAACTTCATGTGTGAGAGGCTGGGTGAGACTCGGAGCCGTG
GGTCCCGACTGAACATCATCATCATCGCTGAGGGTGCCATTGACCGCAACGGGAAGCCCA
TCTCGTCCAGCTACGTGAAGGACCTGGTGGTTCAGAGGCTGGGCTTCGACACCCGTGTAA
CTGTGCTGGGCCACGTGCAGCGGGGAGGGACGCCCTCTGCCTTCGACCGGATCCTGAGCA
GCAAGATGGGCATGGAGGCGGTGATGGCGCTGCTGGAAGCCACGCCTGACACGCCGGCCT
GCGTGGTCACCCTCTCGGGGAACCAGTCAGTGCGGCTGCCCCTCATGGAGTGCGTGCAGA
TGACCAAGGAAGTGCAGAAAGCCATGGATGACAAGAGGTTTGACGAGGCCACCCAGCTCC
GTGGTGGGAGCTTCGAGAACAACTGGAACATTTACAAGCTCCTCGCCCACCAGAAGCCCC
CCAAGGAGAAGTCTAACTTCTCCCTGGCCATCCTGAATGTGGGGGCCCCGGCGGCTGGCA
TGAATGCGGCCGTGCGCTCGGCGGTGCGGACCGGCATCTCCCATGGACACACAGTATACG
TGGTGCACGATGGCTTCGAAGGCCTAGCCAAGGGTCAGGTGCAAGAAGTAGGCTGGCACG
ACGTGGCCGGCTGGTTGGGGCGTGGTGGCTCCATGCTGGGGACCAAGAGGACCCTGCCCA
AGGGCCAGCTGGAGTCCATTGTGGAGAACATCCGCATCTATGGTATTCACGCCCTGCTGG
TGGTCGGTGGGTTTGAGGCCTATGAAGGGGTGCTGCAGCTGGTGGAGGCTCGCGGGCGCT
ACGAGGAGCTCTGCATCGTCATGTGTGTCATCCCAGCCACCATCAGCAACAACGTCCCTG
GCACCGACTTCAGCCTGGGCTCCGACACTGCTGTAAATGCCGCCATGGAGAGCTGTGACC
GCATCAAACAGTCTGCCTCGGGGACCAAGCGCCGTGTGTTCATCGTGGAGACCATGGGGG
GTTACTGTGGCTACCTGGCCACCGTGACTGGCATTGCTGTGGGGGCCGACGCCGCCTACG
TCTTCGAGGACCCTTTCAACATCCACGACTTAAAGGTCAACGTGGAGCACATGACGGAGA
AGATGAAGACAGACATTCAGAGGGGCCTGGTGCTGCGGAACGAGAAGTGCCATGACTACT
ACACCACGGAGTTCCTGTACAACCTGTACTCATCAGAGGGCAACGGCGTCTTCGACTGCA
GGACCAATGTCCTGGGCCACCTGCAGCAGGGTGGCGCTCCAACCCCCTTTGACCGGAACT
ATGGGACCAAGCTGGGGGTGAAGGCCATGCTGTGGTTGTCGGAGAAGCTGCGCGAGGTTT
ACCGCAAGGGACGGGTGTTCGCCAATGCCCCAGACTCGGCCTGCGTGATCGGCCTGAAGA
AGAAGGCGGCGGCCTTCAGCCCCGTCACTGAGCTCAAGAAAGACACTGATTTCGAGCACC
GCATGCCACGGGAGCAGTGGTGGCTGAGCCTGCGGCTCATGCTGAAGATGCTGGCACAAT
ACCGCATCAGTATGGCCGCCTACGTGTCAGGGGAGCTGGAGCACGTGACCCGCCGCACCC
TGAGCATGGACAAGGGCTTCTGAGGCCAGCCATGCCCACGCCCCTCCCCAGCCCCCACCC
ATGCCAGCGCAGCGCCAGGGCTCAGATGGGGCCTGGGCTGTTGTGTCTGGAGCCTGCAGG
CAGGTGGGGGCTGCGTCCCTGCTCAGCCCATCCCCTGCCTCTATCCCTGGCCACCTGCCA
GGCCTCCCTCCGGCTGGTGTCTTGAGACCAGCCTGCCAGGCCCTCCAGCAGGAGGACAGA
GTGCCCTGGGGCATCCACCTTCCTGCCCAGGGGACGTGGCGCTGTCGGTGTTTGGAGGCT
GCTGCCCCCTGGCTTTGGCGCCCCATGGGCCCTCAGCGTCTCCCCATGCTGGGCTCACTA
CATGGGCCAGCCCTTGCTCTACCTGGCCGGTAGGCTGCTGGCGCCTAGGTTGTGTTGAGA
GGGGGATGCCCCTGGCCCTGCCTCACTGTGACCTGCTCCTGCCCACGTGCAGCACCTGTC
ACCTTTTCTAGAAATAAAATCACCCTGACTGTGGGGTGCATCGGTCTCCGGAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites Please inquire     
ACCN NM_002626
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_002626.4, NP_002617.3
RefSeq Size 2924 bp
RefSeq ORF 2343 bp
Locus ID 5211
Cytogenetics 21q22.3
Domains PFK
Protein Families Druggable Genome
Protein Pathways Fructose and mannose metabolism, Galactose metabolism, Glycolysis / Gluconeogenesis, Metabolic pathways, Pentose phosphate pathway
Gene Summary 'This gene encodes the liver (L) subunit of an enzyme that catalyzes the conversion of D-fructose 6-phosphate to D-fructose 1,6-bisphosphate, which is a key step in glucose metabolism (glycolysis). This enzyme is a tetramer that may be composed of different subunits encoded by distinct genes in different tissues. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014]'
Transcript Variant: This variant (2) lacks three alternate exons in the 5' region and initiates translation at an alternate upstream start codon, compared to variant 1. The encoded isoform (b) has a distinct N-terminus and is longer than isoform a.

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