Cathepsin H (CTSH) (NM_004390) Human Untagged Clone

CAT#: SC319365

CTSH (untagged)-Human cathepsin H (CTSH)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CTSH
• Synonyms: ACC-4; ACC-5; ACC4; ACC5; CPSB
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004390.2 GACGCTCTGGGCCGCCACCTCCGCGGACCCTGAGCGCAAGAGCCAAGCCGCCAGCGCTGC
  TATGTGGGCCACGCTGCCGCTGCTCTGCGCCGGGGCCTGGCTCCTGGGAGTCCCCGTCTG
  CGGTGCCGCCGAACTGTCCGTGAACTCCTTAGAGAAGTTTCACTTCAAGTCATGGATGTC
  TAAGCACCGTAAGACCTACAGTACGGAGGAGTACCACCACAGGCTGCAGACGTTTGCCAG
  CAACTGGAGGAAGATAAACGCCCACAACAATGGGAACCACACATTTAAAATGGCACTGAA
  CCAATTTTCAGACATGAGCTTTGCTGAAATAAAACACAAGTATCTCTGGTCAGAGCCTCA
  GAATTGCTCAGCCACCAAAAGTAACTACCTTCGAGGTACTGGTCCCTACCCACCTTCCGT
  GGACTGGCGGAAAAAAGGAAATTTTGTCTCACCTGTGAAAAATCAGGGTGCCTGCGGCAG
  TTGCTGGACTTTCTCCACCACTGGGGCCCTGGAGTCTGCGATCGCCATCGCAACCGGAAA
  GATGCTGTCCTTGGCGGAACAGCAGCTGGTGGACTGCGCCCAGGACTTCAATAATCACGG
  CTGCCAAGGGGGTCTCCCCAGCCAGGCTTTCGAGTATATCCTGTACAACAAGGGGATCAT
  GGGTGAAGACACCTACCCCTACCAGGGCAAGGATGGTTATTGCAAGTTCCAACCTGGAAA
  GGCCATCGGCTTTGTCAAGGATGTAGCCAACATCACAATCTATGACGAGGAAGCGATGGT
  GGAGGCTGTGGCCCTCTACAACCCTGTGAGCTTTGCCTTTGAGGTGACTCAGGACTTCAT
  GATGTATAGAACGGGCATCTACTCCAGTACTTCCTGCCATAAAACTCCAGATAAAGTAAA
  CCATGCAGTACTTGCTGTTGGGTATGGAGAAAAAAATGGGATCCCTTACTGGATCGTGAA
  AAACTCTTGGGGTCCCCAGTGGGGAATGAACGGGTACTTCCTCATCGAGCGCGGAAAGAA
  CATGTGTGGCCTGGCTGCCTGCGCCTCCTACCCCATCCCTCTGGTGTGAGCCGTGGCAGC
  CGCAGCGCAGACTGGCGGAGAAGGAGAGGAACGGGCAGCCTGGGCCTGGGTGGAAATCCT
  GCCCTGGAAGAAGTTGTGGGGAGATCCACTGGGACCCCCAACATTCTGCCCTCACCTCTG
  TGCCCAGCCTGGAAACCTACAGACAAGGAGGAGTTCCACCATGAGCTCACCCGTGTCTAT
  GACGCAAAGATCACCAGCCATGTGCCTTAGTGTCCTTCTTAACAGACTCAAACCACATGG
  ACCACGAATATTCTTTCTGTCCAGAAGGGCTACTTTCCACATATAGAGCTCCAGGGACTG
  TCTTTTCTGTATTCGCTGTTCAATAAACATTGAGTGAGCACCTCCCCAGATGGAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_004390
• Insert Size: 1500 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004390.2, NP_004381.2
• RefSeq Size: 1504 bp
• RefSeq ORF: 1008 bp
• Locus ID: 1512
• Cytogenetics: 15q25.1
• Domains: Pept_C1
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Lysosome
• Gene Summary: 'The protein encoded by this gene is a lysosomal cysteine proteinase important in the overall degradation of lysosomal proteins. It is composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. The encoded protein, which belongs to the peptidase C1 protein family, can act both as an aminopeptidase and as an endopeptidase. Increased expression of this gene has been correlated with malignant progression of prostate tumors. Alternate splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]'
  Transcript Variant: This variant (1) represents the shorter transcript and encodes the longer isoform (a).