TCN2 (NM_000355) Human Untagged Clone

CAT#: SC319392

TCN2 (untagged)-Human transcobalamin II (TCN2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TCN2
• Synonyms: D22S676; D22S750; II; TC; TC-2; TC2; TC II; TCII
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000355.2 GATTAATCAGTGACAGGAAGCTGCGTCTCTCGGAGCGGTGACCAGCTGTGGTCAGGAGAG
  CCTCAGCAGGGCCAGCCCCAGGAGTCTTTCCCGATTCTTGCTCACTGCTCACCCACCTGC
  TGCTGCCATGAGGCACCTTGGGGCCTTCCTCTTCCTTCTGGGGGTCCTGGGGGCCCTCAC
  TGAGATGTGTGAAATACCAGAGATGGACAGCCATCTGGTAGAGAAGTTGGGCCAGCACCT
  CTTACCTTGGATGGACCGGCTTTCCCTGGAGCACTTGAACCCCAGCATCTATGTGGGCCT
  ACGCCTCTCCAGTCTGCAGGCTGGGACCAAGGAAGACCTCTACCTGCACAGCCTCAAGCT
  TGGTTACCAGCAGTGCCTCCTAGGGTCTGCCTTCAGCGAGGATGACGGTGACTGCCAGGG
  CAAGCCTTCCATGGGCCAGCTGGCCCTCTACCTGCTCGCTCTCAGAGCCAACTGTGAGTT
  TGTCAGGGGCCACAAGGGGGACAGGCTGGTCTCACAGCTCAAATGGTTCCTGGAGGATGA
  GAAGAGAGCCATTGGGCATGATCACAAGGGCCACCCCCACACTAGCTACTACCAGTATGG
  CCTGGGCATTCTGGCCCTGTGTCTCCACCAGAAGCGGGTCCATGACAGCGTGGTGGACAA
  ACTTCTGTATGCTGTGGAACCTTTCCACCAGGGCCACCATTCTGTGGACACAGCAGCCAT
  GGCAGGCTTGGCATTCACCTGTCTGAAGCGCTCAAACTTCAACCCTGGTCGGAGACAACG
  GATCACCATGGCCATCAGAACAGTGCAAGAGGAGATCTTGAAGGCCCAGACCCCCGAGGG
  CCACTTTGGGAATGTCTACAGCACCCCATTGGCATTACAGTTCCTCATGACTTCCCCCAT
  GCGTGGGGCAGAACTGGGAACAGCATGTCTCAAGGCGAGGGTTGCTTTGCTGGCCAGTCT
  GCAGGATGGAGCCTTCCAGAATGCTCTCATGATTTCCCAGCTGCTGCCCGTTCTGAACCA
  CAAGACCTACATTGATCTGATCTTCCCAGACTGTCTGGCACCACGAGTCATGTTGGAACC
  AGCTGCTGAGACCATTCCTCAGACCCAAGAGATCATCAGTGTCACGCTGCAGGTGCTTAG
  TCTCTTGCCGCCGTACAGACAGTCCATCTCTGTTCTGGCCGGGTCCACCGTGGAAGATGT
  CCTGAAGAAGGCCCATGAGTTAGGAGGATTCACATATGAAACACAGGCCTCCTTGTCAGG
  CCCCTACTTAACCTCCGTGATGGGGAAAGCGGCCGGAGAAAGGGAGTTCTGGCAGCTTCT
  CCGAGACCCCAACACCCCACTGTTGCAAGGTATTGCTGACTACAGACCCAAGGATGGAGA
  AACCATTGAGCTGAGGCTGGTTAGCTGGTAGCCCCTGAGCTCCCTCATCCCAGCAGCCTC
  GCACACTCCCTAGGCTTCTACCCTCCCTCCTGATGTCCCTGGAACAGGAACTCGCCTGAC
  CCTGCTGCCACCTCCTGTGCACTTTGAGCAATGCCCCCTGGGATCACCCCAGCCACAAGC
  CCTTCGAGGGCCCTATACCATGGCCCACCTTGGAGCAGAGAGCCAAGCATCTTCCCTGGG
  AAGTCTTTCTGGCCAAGTCTGGCCAGCCTGGCCCTGCAGGTCTCCCATGAAGGCCACCCC
  ATGGTCTGATGGGCATGAAGCATCTCAGACTCCTTGGCAAAAAACGGAGTCCGCAGGCCG
  CAGGTGTTGTGAAGACCACTCGTTCTGTGGTTGGGGTCCTGCAAGAAGGCCTCCTCAGCC
  CGGGGGCTATGGCCCTGACCCCAGCTCTCCACTCTGCTGTTAGAGTGGCAGCTCCGAGCT
  GGTTGTGGCACAGTAGCTGGGGAGACCTCAGCAGGGCTGCTCAGTGCCTGCCTCTGACAA
  AATTAAAGCATTGATGGCCTGTGGACCTGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AA
• Restriction Sites: Please inquire
• ACCN: NM_000355
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000355.2, NP_000346.2
• RefSeq Size: 1987 bp
• RefSeq ORF: 1284 bp
• Locus ID: 6948
• Cytogenetics: 22q12.2
• Domains: Cobalamin_bind
• Protein Families: Druggable Genome, Secreted Protein
• Gene Summary: 'This gene encodes a member of the vitamin B12-binding protein family. This family of proteins, alternatively referred to as R binders, is expressed in various tissues and secretions. This plasma protein binds cobalamin and mediates the transport of cobalamin into cells. This protein and other mammalian cobalamin-binding proteins, such as transcobalamin I and gastric intrisic factor, may have evolved by duplication of a common ancestral gene. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010]'
  Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.