NAGA (NM_000262) Human Untagged Clone

CAT#: SC319393

NAGA (untagged)-Human N-acetylgalactosaminidase, alpha- (NAGA)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NAGA
• Synonyms: D22S674; GALB
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000262.1 CTTCGCTTCCAGAGCCCAACACATACAGCTGATACACGCAGACCAGATCTGGTCAGGTCC
  TCGGAAGCTGAGTCCAGAGCGATGCTGCTGAAGACAGTGCTCTTGCTGGGACATGTGGCC
  CAGGTGCTGATGCTGGACAATGGGCTCCTGCAGACACCACCCATGGGCTGGCTGGCCTGG
  GAACGCTTCCGCTGCAACATTAACTGTGATGAGGACCCAAAGAACTGCATAAGTGAACAG
  CTCTTCATGGAGATGGCTGACCGGATGGCACAGGATGGATGGCGGGACATGGGCTACACA
  TACCTCAACATTGATGACTGCTGGATCGGTGGTCGCGATGCCAGTGGCCGCCTGATGCCA
  GATCCCAAGCGCTTCCCTCATGGCATTCCTTTCCTGGCTGACTACGTTCACTCCCTGGGC
  CTGAAGTTGGGTATCTACGCGGACATGGGCAACTTCACCTGCATGGGTTACCCAGGCACC
  ACACTGGACAAGGTGGTCCAGGATGCTCAGACCTTCGCCGAGTGGAAGGTAGACATGCTC
  AAGCTGGATGGCTGCTTCTCCACCCCCGAGGAGCGGGCCCAGGGGTACCCCAAGATGGCT
  GCTGCCCTGAATGCCACAGGCCGCCCCATCGCCTTCTCCTGCAGCTGGCCAGCCTATGAA
  GGCGGCCTCCCCCCAAGGGTGAACTACAGTCTGCTGGCGGACATCTGCAACCTCTGGCGT
  AACTATGATGACATCCAGGACTCCTGGTGGAGCGTGCTCTCCATCCTGAATTGGTTCGTG
  GAGCACCAGGACATACTGCAGCCAGTGGCCGGCCCTGGGCACTGGAATGACCCTGACATG
  CTGCTCATTGGGAACTTTGGTCTCAGCTTAGAGCAATCCCGGGCCCAGATGGCCCTGTGG
  ACGGTGCTGGCAGCCCCCCTCTTGATGTCCACAGACCTGCGTACCATCTCCGCCCAGAAC
  ATGGACATTCTGCAGAATCCACTCATGATCAAAATCAACCAGGATCCCTTAGGCATCCAG
  GGACGCAGGATTCACAAGGAAAAATCTCTCATCGAAGTGTACATGCGGCCTCTGTCCAAC
  AAGGCTAGCGCCTTAGTCTTCTTCAGCTGCAGGACCGATATGCCTTATCGCTACCACTCC
  TCCCTTGGCCAGCTGAACTTCACCGGGTCTGTGATATATGAGGCCCAGGACGTCTACTCA
  GGTGACATCATCAGTGGCCTCCGAGATGAAACCAACTTCACAGTGATCATCAACCCTTCA
  GGGGTAGTGATGTGGTACCTGTATCCCATCAAGAACCTGGAGATGTCCCAGCAGTGAGGA
  GCTGGGACATGTGACAGGCTGTGGTGGCACCACTGAGCCTAGACCATGGAGCCTTGGCAT
  GCCCAGGGCAAGTGGGGAGGTTCTCTGCTCCCCAGGCCTGCTCGGTGACTGACCCCATCA
  TACCCAAAGTGCAATCTCACGGCCAGGTTCTATGCCCTGTCCAAGCGTAAACCCTCTTGG
  AAACTTCTTTTGGGGCAATTTTCCTGTGGCCTTCCTGGCCTCTACTTCCATGTGCGCAGC
  CCCACAGACGTTGCTGAGCAACTCGCCAGCCTCCTGAGCTCCATGCCCATCAGGACTCTA
  GCCTCTGACCTTGCTGTTGACTCTGAAATCAGGATTTGGAAGTTTTCGAATTAGGAGTAG
  AGAGATCTGACCTCTTGCCAGGAATGCCCATGGATCATGTGATTGGCTTTTCTACCCATA
  GAGGGCCTTGCAGCCTGATACCACCTGGGAGTGAGGGTCACAAAGGAGACCTTGGCTCCC
  TCAGGTCACCAATAAACCTGTTCTTTAATCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAA
• Restriction Sites: Please inquire
• ACCN: NM_000262
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000262.1, NP_000253.1
• RefSeq Size: 3598 bp
• RefSeq ORF: 1236 bp
• Locus ID: 4668
• Cytogenetics: 22q13.2
• Domains: Melibiase
• Protein Families: Druggable Genome
• Protein Pathways: Glycosphingolipid biosynthesis - globo series, Lysosome
• Gene Summary: 'NAGA encodes the lysosomal enzyme alpha-N-acetylgalactosaminidase, which cleaves alpha-N-acetylgalactosaminyl moieties from glycoconjugates. Mutations in NAGA have been identified as the cause of Schindler disease types I and II (type II also known as Kanzaki disease). [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the longest transcript variant. Variants 1-3 encode the same protein.