FADS3 (NM_021727) Human Untagged Clone

CAT#: SC319457

FADS3 (untagged)-Human fatty acid desaturase 3 (FADS3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FADS3
• Synonyms: CYB5RP; LLCDL3
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_021727.3 CGCACGCTCGCTCGTACGGCGGCCGCGGCGGCAGGGCGGGGCCGGAGCAGCGGGCGGCGG
  CGGAGGCGGCGCCCGGGAGCGCTCTTCGCTTCCCTCGGGGTCTTGCTCGGACCTCGGCCA
  CCGCCTGGGATCCCCAGGACTCGTGCGTGCAGCATGGGCGGCGTCGGGGAGCCGGGACCG
  CGGGAGGGACCCGCGCAGCCGGGGGCACCGCTGCCCACCTTCTGCTGGGAGCAGATCCGC
  GCGCACGACCAGCCCGGCGACAAGTGGCTGGTCATCGAGCGCCGCGTCTACGACATCAGC
  CGCTGGGCACAGCGGCACCCAGGGGGCAGCCGCCTCATCGGCCACCACGGCGCTGAGGAC
  GCCACGGATGCCTTCCGTGCCTTCCATCAAGATCTCAATTTTGTGCGCAAGTTCCTACAG
  CCCCTGTTGATTGGAGAGCTGGCTCCGGAAGAACCCAGCCAGGATGGACCCCTGAATGCG
  CAGCTGGTCGAGGACTTCCGAGCCCTGCACCAGGCAGCCGAGGACATGAAGCTGTTTGAT
  GCCAGTCCCACCTTCTTTGCTTTCCTACTGGGCCACATCCTGGCCATGGAGGTGCTGGCC
  TGGCTCCTTATCTACCTCCTGGGTCCTGGCTGGGTGCCCAGTGCCCTGGCCGCCTTCATC
  CTGGCCATCTCTCAGGCTCAGTCCTGGTGTCTGCAGCATGACCTGGGCCATGCCTCCATC
  TTCAAGAAGTCCTGGTGGAACCACGTGGCCCAGAAGTTCGTGATGGGGCAGCTAAAGGGC
  TTCTCCGCCCACTGGTGGAACTTCCGCCACTTCCAGCACCACGCCAAGCCCAACATCTTC
  CACAAAGACCCAGACGTGACGGTGGCGCCCGTCTTCCTCCTGGGGGAGTCATCCGTCGAG
  TATGGCAAGAAGAAACGCAGATACCTACCCTACAACCAGCAGCACCTGTACTTCTTCCTG
  ATCGGCCCGCCGCTGCTCACCCTGGTGAACTTTGAAGTGGAAAATCTGGCGTACATGCTG
  GTGTGCATGCAGTGGGCGGATTTGCTCTGGGCCGCCAGCTTCTATGCCCGCTTCTTCTTA
  TCCTACCTCCCCTTCTACGGCGTCCCTGGGGTGCTGCTCTTCTTTGTTGCTGTCAGGGTC
  CTGGAAAGCCACTGGTTCGTGTGGATCACACAGATGAACCACATCCCCAAGGAGATCGGC
  CACGAGAAGCACCGGGACTGGGTCAGCTCTCAGCTGGCAGCCACCTGCAACGTGGAGCCC
  TCACTTTTCACCAACTGGTTCAGCGGGCACCTCAACTTCCAGATCGAGCACCACCTCTTC
  CCCAGGATGCCGAGACACAACTACAGCCGGGTGGCCCCGCTGGTCAAGTCGCTGTGTGCC
  AAGCACGGCCTCAGCTACGAAGTGAAGCCCTTCCTCACCGCGCTGGTGGACATCGTCAGG
  TCCCTGAAGAAGTCTGGTGACATCTGGCTGGACGCCTACCTCCATCAGTGAAGGCAACAC
  CCAGGCGGGCAGAGAAGGGCTCAGGGCACCAGCAACCAAGCCAGCCCCCGGCGGGATCGA
  TACCCCCACCCCTCCACTGGCCAGCCTGGGGGTGCCCTGCCTGCCCTCCTGGTACTGTTG
  TCTTCCCCTCGGCCCCCTCACATGTGTATTCAGCAGCCCTATGGCCTTGGCTCTGGGCCT
  GATGGGACAGGGGTAGAGGGAAGGTGAGCATAGCACATTTTCCTAGAGCGAGAATTGGGG
  GAAAGCTGTTATTTTTATATTAAAATACATTCAGATGTAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_021727
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021727.3, NP_068373.1
• RefSeq Size: 1807 bp
• RefSeq ORF: 1338 bp
• Locus ID: 3995
• Cytogenetics: 11q12.2
• Domains: heme_1, FA_desaturase
• Protein Families: Transmembrane
• Gene Summary: 'The protein encoded by this gene is a member of the fatty acid desaturase (FADS) gene family. Desaturase enzymes regulate unsaturation of fatty acids through the introduction of double bonds between defined carbons of the fatty acyl chain. FADS family members are considered fusion products composed of an N-terminal cytochrome b5-like domain and a C-terminal multiple membrane-spanning desaturase portion, both of which are characterized by conserved histidine motifs. This gene is clustered with family members FADS1 and FADS2 at 11q12-q13.1; this cluster is thought to have arisen evolutionarily from gene duplication based on its similar exon/intron organization. [provided by RefSeq, Jul 2008]'