UROD (NM_000374) Human Untagged Clone

CAT#: SC319474

UROD (untagged)-Human uroporphyrinogen decarboxylase (UROD), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: UROD
• Synonyms: PCT; UPD
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000374.3 TTGAGCTCGCAGTTACAGACAGCTGACCATGGAAGCGAATGGGTTGGGACCTCAGGGTTT
  TCCGGAGCTGAAGAATGACACATTCCTGCGAGCAGCCTGGGGAGAGGAAACAGACTACAC
  TCCCGTTTGGTGCATGCGCCAGGCAGGCCGTTACTTACCAGAGTTTAGGGAAACCCGGGC
  TGCCCAGGACTTTTTCAGCACGTGTCGCTCTCCTGAGGCCTGCTGTGAACTGACTCTGCA
  GCCACTGCGTCGCTTCCCTCTGGATGCTGCCATCATTTTCTCCGACATCCTTGTTGTACC
  CCAGGCACTGGGCATGGAGGTGACCATGGTACCTGGCAAAGGACCCAGCTTCCCAGAGCC
  ATTAAGAGAAGAGCAGGACCTAGAACGCCTACGGGATCCAGAAGTGGTAGCCTCTGAGCT
  AGGCTATGTGTTCCAAGCCATCACCCTTACCCGACAACGACTGGCTGGACGTGTGCCGCT
  GATTGGCTTTGCTGGTGCCCCATGGACCCTGATGACATACATGGTTGAGGGTGGTGGCTC
  AAGCACCATGGCTCAGGCCAAGCGCTGGCTCTATCAGAGACCTCAGGCTAGTCACCAGCT
  GCTTCGCATCCTCACTGATGCTCTGGTCCCATATCTGGTAGGACAAGTGGTGGCTGGTGC
  CCAGGCATTGCAGCTGTTTGAGTCCCATGCAGGGCATCTTGGCCCACAGCTCTTCAACAA
  GTTTGCACTGCCTTACATCCGTGATGTGGCCAAGCAAGTGAAGGCCAGGTTGCGGGAGGC
  AGGCCTGGCACCAGTGCCCATGATCATCTTTGCTAAGGATGGGCATTTTGCCCTGGAGGA
  GCTGGCCCAAGCTGGCTATGAGGTGGTTGGGCTTGACTGGACAGTGGCCCCAAAGAAAGC
  CCGGGAGTGTGTGGGGAAGACGGTGACATTGCAGGTCAACCTGGACCCCTGTGCCTTGTA
  TGCATCTGAGGAGGAGATCGGGCAGTTGGTGAAGCAGATGCTGGATGACTTTGGACCACA
  TCGCTACATTGCCAACCTGGGCCATGGGCTTTATCCTGACATGGACCCAGAACATGTGGG
  CGCCTTTGTGGATGCTGTGCATAAACACTCACGTCTGCTTCGACAGAACTGAGTGTATAC
  CTTTACCCTCAAGTACCACTAACACAGATGATTGATCGTTTCCAGGACAATAAAAGTTTC
  GGAGTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAA
• Restriction Sites: Please inquire
• ACCN: NM_000374
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000374.3, NP_000365.3
• RefSeq Size: 1383 bp
• RefSeq ORF: 1104 bp
• Locus ID: 7389
• Cytogenetics: 1p34.1
• Domains: URO-D
• Protein Families: Druggable Genome
• Protein Pathways: Porphyrin and chlorophyll metabolism
• Gene Summary: 'This gene encodes an enzyme in the heme biosynthetic pathway. This enzyme is responsible for catalyzing the conversion of uroporphyrinogen to coproporphyrinogen through the removal of four carboxymethyl side chains. Mutations and deficiency in this enzyme are known to cause familial porphyria cutanea tarda and hepatoerythropoetic porphyria.[provided by RefSeq, Aug 2010]'
  Transcript Variant: This variant (1) represents the shortest transcript and encodes the functional protein.