BCAT2 (NM_001190) Human Untagged Clone

CAT#: SC319477

BCAT2 (untagged)-Human branched chain amino-acid transaminase 2, mitochondrial (BCAT2), nuclear gene encoding mitochondrial protein, transcript variant a

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BCAT2
• Synonyms: BCAM; BCATM; BCT2; PP18
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001190.2 CGCCGCACGGATCATGGCCGCAGCCGCTCTGGGGCAGATCTGGGCACGAAAGCTTCTCTC
  TGTCCCTTGGCTTCTGTGTGGTCCCAGAAGATATGCCTCCTCCAGTTTCAAGGCTGCAGA
  CCTGCAGCTGGAAATGACACAGAAGCCTCATAAGAAGCCTGGCCCCGGCGAGCCCCTGGT
  GTTTGGGAAGACATTTACCGACCACATGCTGATGGTGGAATGGAATGACAAGGGCTGGGG
  CCAGCCCCGAATCCAGCCCTTCCAGAACCTCACGCTGCACCCAGCCTCCTCCAGCCTCCA
  CTACTCCCTGCAGCTGTTTGAGGGCATGAAGGCGTTCAAAGGCAAAGACCAGCAGGTGCG
  CCTCTTCCGCCCCTGGCTCAACATGGACCGGATGCTGCGCTCAGCCATGCGCCTGTGCCT
  GCCGAGTTTCGACAAGCTGGAGTTGCTGGAGTGCATCCGCCGGCTCATCGAAGTGGACAA
  GGACTGGGTCCCCGATGCCGCCGGCACCAGCCTCTATGTGCGGCCTGTGCTCATTGGGAA
  CGAGCCCTCGCTGGGTGTCAGCCAGCCCACGCGCGCGCTCCTGTTCGTCATTCTCTGCCC
  AGTGGGTGCCTACTTCCCTGGAGGCTCCGTGACCCCGGTCTCCCTCCTGGCCGACCCAGC
  CTTCATCCGGGCCTGGGTGGGCGGGGTCGGCAACTACAAGTTAGGTGGGAATTATGGGCC
  CACCGTGTTAGTGCAACAGGAGGCACTCAAGCGGGGCTGTGAACAGGTCCTCTGGCTGTA
  TGGGCCCGACCACCAGCTCACCGAGGTGGGAACCATGAACATCTTTGTCTACTGGACCCA
  CGAAGATGGGGTGCTGGAGCTGGTGACGCCCCCGCTGAATGGTGTTATCCTGCCTGGAGT
  GGTCAGACAGAGTCTACTGGACATGGCTCAGACCTGGGGTGAGTTCCGGGTGGTGGAGCG
  CACGATCACCATGAAGCAGTTGCTGCGGGCCCTGGAGGAGGGCCGCGTGCGGGAAGTCTT
  TGGCTCGGGCACCGCTTGCCAGGTCTGCCCAGTGCACCGAATCCTGTACAAAGACAGGAA
  CCTCCACATTCCCACCATGGAAAATGGGCCTGAGCTGATCCTCCGCTTCCAGAAGGAGCT
  GAAGGAGATCCAGTACGGAATCAGAGCCCACGAGTGGATGTTCCCGGTGTGAAGCTGCAG
  GCTGTGCTCCAGATCCACCGACCCGTAGCATCTCGTCACGCCAGCACTCGCCTCCCTACC
  AATGACTCACCTGAAGTGCAATACGAAATAAAAGGCCAGCGGGCGGCGTCTGGGTCTCTG
  GCGCCCCCATGTGGTTGCGACACTCCCAAAGCCGTAAGGGCCGACCCAGGCATCTTGGCC
  CCCAGCCCCTCGTCGCGGGTTCAGGTCCGCCCATTACTCCTTGTCGTGCGGTCAAGGATA
  CACCTTGGCCCCGATTCCGGATCTCTCCGTTCTCAGGCCAGACCCCTGGTGCTGCCGTTG
  ATTTTTTTTTCTCTGTCTTTGCTGCAATTTTGAAATAAAATGCCAAAGAACAAAAAAAAA
  AAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_001190
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001190.2, NP_001181.2
• RefSeq Size: 1595 bp
• RefSeq ORF: 1179 bp
• Locus ID: 587
• Cytogenetics: 19q13.33
• Domains: aminotran_4
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Pantothenate and CoA biosynthesis, Valine, leucine and isoleucine biosynthesis, Valine, leucine and isoleucine degradation
• Gene Summary: 'This gene encodes a branched chain aminotransferase found in mitochondria. The encoded protein forms a dimer that catalyzes the first step in the production of the branched chain amino acids leucine, isoleucine, and valine. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]'
  Transcript Variant: This variant (a) encodes the longest isoform (a), also known as PP18a.