ECHS1 (NM_004092) Human Untagged Clone

CAT#: SC319501

ECHS1 (untagged)-Human enoyl CoA hydratase, short chain, 1, mitochondrial (ECHS1), nuclear gene encoding mitochondrial protein


  "NM_004092" in other vectors (6)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol ECHS1
Synonyms ECHS1D; SCEH
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>OriGene sequence for NM_004092.2 GAGTCCAGAGAGCCATGGCCGCCCTGCGTGTCCTGCTGTCCTGCGTCCGCGGCCCGCTGA
GGCCCCCGGTTCGCTGTCCCGCCTGGCGTCCCTTCGCCTCGGGTGCTAACTTTGAGTACA
TCATCGCAGAAAAAAGAGGGAAGAATAACACCGTGGGGTTGATCCAACTGAACCGCCCCA
AGGCCCTCAATGCACTTTGCGATGGCCTGATTGACGAGCTCAACCAGGCCCTGAAGATCT
TCGAGGAGGACCCGGCCGTGGGGGCCATTGTCCTCACCGGCGGGGATAAGGCCTTTGCAG
CTGGAGCTGATATCAAGGAAATGCAGAACCTGAGTTTCCAGGACTGTTACTCCAGCAAGT
TCTTGAAGCACTGGGACCACCTCACCCAGGTCAAGAAGCCAGTCATCGCTGCTGTCAATG
GCTATGCCTTTGGCGGGGGCTGTGAGCTTGCCATGATGTGTGATATCATCTATGCCGGTG
AGAAGGCCCAGTTTGCACAGCCGGAGATCTTAATAGGAACCATCCCAGGTGCGGGCGGCA
CCCAGAGACTCACCCGTGCTGTTGGGAAGTCGCTGGCGATGGAGATGGTCCTCACCGGTG
ACCGGATCTCAGCCCAGGACGCCAAGCAAGCAGGTCTTGTCAGCAAGATTTGTCCTGTTG
AGACACTGGTGGAAGAAGCCATCCAGTGTGCAGAAAAAATTGCCAGCAATTCTAAAATTG
TAGTAGCGATGGCCAAAGAATCAGTGAATGCAGCTTTTGAAATGACATTAACAGAAGGAA
GTAAGTTGGAGAAGAAACTCTTTTATTCAACCTTTGCCACTGATGACCGGAAAGAAGGGA
TGACCGCGTTTGTGGAAAAGAGAAAGGCCAACTTCAAAGACCAGTGAGAACCAGCTGCCC
CTGCTTCACACCTCTGCTTGGAGAGGACAAGTGCAGCCTGTCAGTTTTAGAAGCAAGTAA
ATCATCCTCTTTTCAAGAGCAGTGTCCGTGGTGTGCAGTTCCTCTCCAATTGCTGCGTGG
TCGTGGCCCGACCTCTCACGGCATGACAGCCTTCGTCACCCAGCCTGTGAGGGTCCTGAC
TGGAGCACCTTCTAAATCTAAGATTCTGCTGAGGAGCCCCCGCTGGTCCCTCTGGGCATG
CTGTGCTCGGACGGAAAGCGGGGCCTGCGGGTCCTTGTGTCCCTGCCGCTGAAGAATGGG
GCTGCTCTGAGGGAAACGCTGTCTGCTGCCTTCATACAGATGCTGATTAAAGTGATAGCG
ATTCAGATTACAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites Please inquire     
ACCN NM_004092
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_004092.2, NP_004083.2
RefSeq Size 1326 bp
RefSeq ORF 873 bp
Locus ID 1892
Cytogenetics 10q26.3
Domains ECH
Protein Pathways beta-Alanine metabolism, Butanoate metabolism, Fatty acid elongation in mitochondria, Fatty acid metabolism, Limonene and pinene degradation, Lysine degradation, Metabolic pathways, Propanoate metabolism, Tryptophan metabolism, Valine, leucine and isoleucine degradation
Gene Summary 'The protein encoded by this gene functions in the second step of the mitochondrial fatty acid beta-oxidation pathway. It catalyzes the hydration of 2-trans-enoyl-coenzyme A (CoA) intermediates to L-3-hydroxyacyl-CoAs. The gene product is a member of the hydratase/isomerase superfamily. It localizes to the mitochondrial matrix. Transcript variants utilizing alternative transcription initiation sites have been described in the literature. [provided by RefSeq, Jul 2008]'

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