TTF1 (NKX2-1) (NM_001079668) Human Untagged Clone

CAT#: SC319511

NKX2 (untagged)-Human NK2 homeobox 1 (NKX2-1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NKX2-1
• Synonyms: BCH; BHC; NK-2; NKX2.1; NKX2A; NMTC1; T/EBP; TEBP; TITF1; TTF-1; TTF1
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001079668.1 GGCGACTGGGGCTCAGCGCAGCGAAGCCCGATGTGGTCCGGAGGCAGTGGGAAGGCGCGG
  GGCTGGGAGGCCGCGGCGGGAGGGAGGAGCAGCCCCGGCAGGCTCAGCCGCCGCCGAATC
  ATGTCGATGAGTCCAAAGCACACGACTCCGTTCTCAGTGTCTGACATCTTGAGTCCCCTG
  GAGGAAAGCTACAAGAAAGTGGGCATGGAGGGCGGCGGCCTCGGGGCTCCGCTGGCGGCG
  TACAGGCAGGGCCAGGCGGCACCGCCAACAGCGGCCATGCAGCAGCACGCCGTGGGGCAC
  CACGGCGCCGTCACCGCCGCCTACCACATGACGGCGGCGGGGGTGCCCCAGCTCTCGCAC
  TCCGCCGTGGGGGGCTACTGCAACGGCAACCTGGGCAACATGAGCGAGCTGCCGCCGTAC
  CAGGACACCATGAGGAACAGCGCCTCTGGCCCCGGATGGTACGGCGCCAACCCAGACCCG
  CGCTTCCCCGCCATCTCCCGCTTCATGGGCCCGGCGAGCGGCATGAACATGAGCGGCATG
  GGCGGCCTGGGCTCGCTGGGGGACGTGAGCAAGAACATGGCCCCGCTGCCAAGCGCGCCG
  CGCAGGAAGCGCCGGGTGCTCTTCTCGCAGGCGCAGGTGTACGAGCTGGAGCGACGCTTC
  AAGCAACAGAAGTACCTGTCGGCGCCGGAGCGCGAGCACCTGGCCAGCATGATCCACCTG
  ACGCCCACGCAGGTCAAGATCTGGTTCCAGAACCACCGCTACAAAATGAAGCGCCAGGCC
  AAGGACAAGGCGGCGCAGCAGCAACTGCAGCAGGACAGCGGCGGCGGCGGGGGCGGCGGG
  GGCACCGGGTGCCCGCAGCAGCAACAGGCTCAGCAGCAGTCGCCGCGACGCGTGGCGGTG
  CCGGTCCTGGTGAAAGACGGCAAACCGTGCCAGGCGGGTGCCCCCGCGCCGGGCGCCGCC
  AGCCTACAAGGCCACGCGCAGCAGCAGGCGCAGCACCAGGCGCAGGCCGCGCAGGCGGCG
  GCAGCGGCCATCTCCGTGGGCAGCGGTGGCGCCGGCCTTGGCGCACACCCGGGCCACCAG
  CCAGGCAGCGCAGGCCAGTCTCCGGACCTGGCGCACCACGCCGCCAGCCCCGCGGCGCTG
  CAGGGCCAGGTATCCAGCCTGTCCCACCTGAACTCCTCGGGCTCGGACTACGGCACCATG
  TCCTGCTCCACCTTGCTATACGGTCGGACCTGGTGAGAGGACGCCGGGCCGGCCCTAGCC
  CAGCGCTCTGCCTCACCGCTTCCCTCCTGCCCGCCACACAGACCACCATCCACCGCTGCT
  CCACGCGCTTCGACTTTTCTTAACAACCTGGCCGCGTTTAGACCAAGGAACAAAAAAACC
  ACAAAGGCCAAACTGCTGGACGTCTTTCTTTTTTTCCCCCCCTAAAATTTGTGGGTTTTT
  TTTTTAAAAAAAAGAAAATGAAAAACAACCAAGCGCATCCAATCTCAAGGAATCTTTAAG
  CAGAGAAGGGCATAAAACAGCTTTGGGGTGTCTTTTTTTGGTGATTCAAATGGGTTTTCC
  ACGCTAGGGCGGGGCACAGATTGGAGAGGGCTCTGTGCTGACATGGCTCTGGACTCTAAA
  GACCAAACTTCACTCTGGGCACACTCTGCCAGCAAAGAGGACTCGCTTGTAAATACCAGG
  ATTTTTTTTTTTTTTTGAAGGGAGGACGGGAGCTGGGGAGAGGAAAGAGTCTTCAACATA
  ACCCACTTGTCACTGACACAAAGGAAGTGCCCCCTCCCCGGCACCCTCTGGCCGCCTAGG
  CTCAGCGGCGACCGCCCTCCGCGAAAATAGTTTGTTTAATGTGAACTTGTAGCTGTAAAA
  CGCTGTCAAAAGTTGGACTAAATGCCTAGTTTTTAGTAATCTGTACATTTTGTTGTAAAA
  AGAAAAACCACTCCCAGTCCCCAGCCCTTCACATTTTTTATGGGCATTGACAAATCTGTG
  TATATTATTTGGCAGTTTGGTATTTGCGGCGTCAGTCTTTTTCTGTTGTAACTTATGTAG
  ATATTTGGCTTAAATATAGTTCCTAAGAAGCTTCTAATAAATTATACAAATTAAAAAGAT
  TCTTTTTCTGATTAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_001079668
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001079668.1, NP_001073136.1
• RefSeq Size: 2183 bp
• RefSeq ORF: 1206 bp
• Locus ID: 7080
• Cytogenetics: 14q13.3
• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors
• Gene Summary: 'This gene encodes a protein initially identified as a thyroid-specific transcription factor. The encoded protein binds to the thyroglobulin promoter and regulates the expression of thyroid-specific genes but has also been shown to regulate the expression of genes involved in morphogenesis. Mutations and deletions in this gene are associated with benign hereditary chorea, choreoathetosis, congenital hypothyroidism, and neonatal respiratory distress, and may be associated with thyroid cancer. Multiple transcript variants encoding different isoforms have been found for this gene. This gene shares the symbol/alias 'TTF1' with another gene, transcription termination factor 1, which plays a role in ribosomal gene transcription. [provided by RefSeq, Feb 2014]'
  Transcript Variant: This variant (1) encodes the longer protein (isoform 1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.