Helicase SKI2W (SKIV2L) (NM_006929) Human Untagged Clone

CAT#: SC319532

SKIV2L (untagged)-Human superkiller viralicidic activity 2-like (S. cerevisiae) (SKIV2L)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SKIV2L
• Synonyms: 170A; DDX13; HLP; SKI2; SKI2W; SKIV2; SKIV2L1; THES2
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_006929.4 GGATGATGGAGACAGAGCGACTTGTGCTACCCCCTCCAGATCCCCTGGACCTACCCCTTC
  GGGCCGTGGAGCTCGGATGCACGGGGCACTGGGAGCTGCTGAACTTGCCTGGAGCTCCAG
  AGAGTAGCCTTCCCCATGGCCTCCCTCCTTGTGCCCCAGATCTGCAGCAAGAAGCAGAAC
  AGTTGTTTCTGTCATCCCCAGCCTGGCTGCCTCTGCATGGTGTGGAGCACTCAGCCCGAA
  AATGGCAGAGGAAGACGGATCCCTGGTCTCTTTTGGCTGTGCTGGGAGCCCCAGTCCCAT
  CCGACCTACAGGCCCAAAGACACCCAACCACAGGCCAGATACTGGGTTACAAAGAGGTCT
  TGCTGGAGAACACAAATCTCTCGGCTACAACCTCCTTGTCTCTTCGCCGGCCTCCAGGGC
  CAGCCTCCCAGTCCTTATGGGGAAATCCAACTCGGTATCCCTTCTGGCCAGGGGGGATGG
  ATGAACCCACCATAACAGATCTGAACACACGGGAGGAGGCTGAGGAGGAGATAGACTTTG
  AGAAAGATCTTCTTACTATTCCACCTGGTTTCAAGAAAGGCATGGACTTTGCACCAAAAG
  ATTGTCCAACTCCAGCTCCTGGACTACTAAGCCTTAGCTGTCTGTTGGAGCCTCTGGATT
  TGGGTGGGGGTGACGAGGATGAGAATGAGGCAGTGGGACAGCCAGGAGGTCCCAGAGGGG
  ACACTGTTTCAGCCTCTCCCTGCAGTGCTCCCCTGGCCCGAGCAAGCAGCTTGGAAGACC
  TAGTGTTGAAGGAAGCGTCCACAGCTGTATCCACCCCAGAGGCCCCAGAGCCTCCATCTC
  AGGAGCAGTGGGCCATCCCTGTGGACGCCACCTCCCCTGTTGGTGATTTCTATCGCCTCA
  TTCCCCAGCCAGCCTTCCAGTGGGCATTTGAGCCAGATGTGTTTCAGAAACAGGCCATCC
  TGCACTTGGAACGGCATGACTCTGTCTTTGTCGCAGCTCACACATCTGCAGGAAAAACAG
  TTGTGGCTGAATATGCCATTGCCCTGGCCCAGAAACACATGACACGCACCATCTACACTT
  CGCCCATCAAGGCCCTGAGCAACCAGAAGTTCCGGGACTTCCGAAACACATTCGGGGATG
  TGGGGCTGCTCACCGGGGATGTACAGCTGCATCCGGAGGCCTCCTGCCTCATCATGACCA
  CAGAGATCCTTCGCTCCATGCTGTACAGTGGCTCAGATGTTATTCGGGACCTGGAGTGGG
  TCATCTTTGATGAGGTTCACTATATCAACGATGTCGAGCGTGGGGTCGTGTGGGAGGAGG
  TGCTTATCATGCTACCTGACCACGTTTCTATCATCCTTCTGAGTGCCACCGTCCCCAACG
  CCCTTGAGTTTGCTGACTGGATTGGGCGGCTGAAGCGTCGTCAGATCTATGTGATTAGCA
  CTGTAACCCGCCCCGTGCCCCTGGAGCACTATCTTTTCACAGGGAACAGCTCCAAGACCC
  AGGGGGAGCTCTTTTTGTTGCTGGACTCCCGAGGAGCCTTCCATACAAAAGGGTACTATG
  CAGCTGTGGAGGCCAAGAAGGAGAGAATGAGCAAACACGCCCAGACCTTTGGGGCCAAGC
  AGCCCACACATCAGGGGGGCCCTGCACAGGACCGCGGAGTGTACCTGTCCCTCCTGGCCT
  CCCTCCGCACACGTGCCCAGTTGCCCGTGGTGGTGTTCACCTTCTCCCGGGGCCGCTGTG
  ATGAGCAGGCCTCAGGCCTCACCTCCCTTGACCTCACCACCAGTTCGGAGAAGAGCGAGA
  TCCACCTCTTCCTGCAGCGCTGCCTTGCTCGCCTCCGTGGCTCTGACCGCCAGCTGCCCC
  AGGTCCTGCACATGTCAGAGCTCCTGAATCGCGGCCTGGGTGTGCACCATAGCGGCATCC
  TGCCCATCCTCAAGGAGATCGTGGAGATGCTCTTCAGCCGTGGCCTGGTCAAGGTCTTGT
  TTGCCACAGAGACCTTTGCCATGGGAGTAAACATGCCTGCTCGTACAGTAGTGTTTGACT
  CCATGCGCAAACACGATGGCTCCACCTTCCGGGACCTGCTCCCTGGGGAGTATGTGCAGA
  TGGCAGGCCGGGCAGGGCGGAGGGGCCTGGACCCCACAGGCACCGTTATCCTGCTCTGCA
  AGGGCCGAGTGCCCGAGATGGCAGACCTGCACCGCATGATGATGGGGAAGCCGTCCCAGC
  TGCAGTCCCAGTTCCGCCTCACGTACACTATGATCCTCAACTTGCTGCGAGTGGATGCCC
  TCAGGGTGGAGGACATGATGAAGAGGAGCTTCTCTGAGTTTCCCTCCCGCAAAGACAGCA
  AGGCCCATGAACAGGCCCTGGCTGAACTGACCAAGAGGCTGGGAGCTTTGGAGGAGCCTG
  ACATGACTGGCCAACTGGTCGACCTGCCTGAATATTACAGCTGGGGGGAGGAACTGACAG
  AGACCCAGCACATGATCCAGCGACGCATCATGGAGTCTGTGAACGGGCTGAAGTCTCTCT
  CAGCAGGAAGGGTGGTGGTTGTGAAGAATCAGGAGCATCACAACGCATTGGGAGTGATCC
  TACAGGTCTCCTCGAACTCCACCAGCAGAGTATTCACAACCCTGGTCTTGTGTGATAAGC
  CCTTGTCCCAGGACCCACAGGACAGGGGGCCAGCCACTGCAGAGGTGCCCTATCCAGATG
  ACCTCGTGGGATTCAAGCTGTTCCTGCCTGAAGGGCCTTGTGACCACACCGTGGTCAAGC
  TCCAGCCAGGAGATATGGCTGCCATCACCACCAAGGTGCTCCGGGTGAATGGGGAGAAGA
  TCTTGGAGGACTTCAGCAAGAGGCAGCAGCCAAAATTCAAGAAGGATCCTCCCCTTGCAG
  CCGTGACCACTGCTGTCCAGGAACTGCTGCGTCTGGCTCAGGCCCACCCAGCCGGACCTC
  CCACCCTCGACCCTGTCAATGACCTGCAGCTCAAAGATATGTCAGTTGTAGAGGGTGGGC
  TCCGGGCCCGGAAGCTGGAGGAGCTGATCCAGGGGGCTCAGTGTGTACACAGCCCCCGTT
  TTCCTGCCCAGTACCTGAAGCTGCGGGAGCGAATGCAGATACAGAAGGAGATGGAGCGGC
  TGCGCTTCCTACTGTCGGATCAGTCATTGCTGCTGCTTCCTGAGTACCATCAGCGAGTAG
  AGGTGCTCCGAACCCTGGGTTACGTGGACGAGGTGGGCACTGTGAAGCTGGCAGGGCGGG
  TGGCTTGTGCCATGAGCAGCCATGAGTTGCTCCTCACTGAGCTCATGTTTGACAATGCAC
  TGAGCACCCTGCGGCCTGAGGAGATTGCTGCCTTGCTCTCTGGCCTGGTCTGCCAGAGCC
  CTGGGGACGCTGGGGATCAGCTCCCAAACACCCTCAAGCAGGGAATAGAACGTGTCCGGG
  CTGTGGCCAAGCGGATTGGTGAGGTCCAGGTGGCTTGTGGCCTGAACCAGACGGTGGAGG
  AATTTGTGGGGGAGCTGAATTTTGGGCTGGTTGAGGTTGTATATGAGTGGGCCCGGGGCA
  TGCCCTTCTCCGAGTTGGCAGGGCTCTCAGGGACCCCTGAGGGCCTGGTGGTCCGCTGCA
  TTCAGCGCCTGGCTGAGATGTGTCGCTCACTGCGGGGGGCAGCCCGCCTGGTAGGAGAGC
  CTGTGCTGGGTGCCAAGATGGAGACAGCGGCTACCTTGCTACGGCGGGACATCGTATTTG
  CGGCCAGCCTCTACACCCAGTGAATGCCCCATGTAAAAACATGATGATAAAACAGCAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_006929
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006929.4, NP_008860.4
• RefSeq Size: 4187 bp
• RefSeq ORF: 3741 bp
• Locus ID: 6499
• Cytogenetics: 6p21.33
• Domains: DEAD, helicase_C
• Protein Pathways: RNA degradation
• Gene Summary: 'DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which is a human homologue of yeast SKI2 and may be involved in antiviral activity by blocking translation of poly(A) deficient mRNAs. This gene is located in the class III region of the major histocompatibility complex. [provided by RefSeq, Jul 2008]'