ECM1 (NM_004425) Human Untagged Clone

CAT#: SC319537

ECM1 (untagged)-Human extracellular matrix protein 1 (ECM1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ECM1
• Synonyms: URBWD
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004425.2 AGAAGAGCTGGTCCTGAAGCTCACAACCGTAACAGCCACCAGACAAGCTTCAGTGGCCGG
  CCCTTCACATCCAGACTTGCCTGAGAGGACCCACCTCTGAGTGTCCAGTGGTCAGTTGCC
  CCAGGATGGGGACCACAGCCAGAGCAGCCTTGGTCTTGACCTATTTGGCTGTTGCTTCTG
  CTGCCTCTGAGGGAGGCTTCACGGCTACAGGACAGAGGCAGCTGAGGCCAGAGCACTTTC
  AAGAAGTTGGCTACGCAGCTCCCCCCTCCCCACCCCTATCCCGAAGCCTCCCCATGGATC
  ACCCTGACTCCTCTCAGCATGGCCCTCCCTTTGAGGGACAGAGTCAAGTGCAGCCCCCTC
  CCTCTCAGGAGGCCACCCCTCTCCAACAGGAAAAGCTGCTACCTGCCCAACTCCCTGCTG
  AAAAGGAAGTGGGTCCCCCTCTCCCTCAGGAAGCTGTCCCCCTCCAAAAAGAGCTGCCCT
  CTCTCCAGCACCCCAATGAACAGAAGGAAGGAATGCCAGCTCCATTTGGGGACCAGAGCC
  ATCCAGAACCTGAGTCCTGGAATGCAGCCCAGCACTGCCAACAGGACCGGTCCCAAGGGG
  GCTGGGGCCACCGGCTGGATGGCTTCCCCCCTGGGCGGCCTTCTCCAGACAATCTGAACC
  AAATCTGCCTTCCTAACCGTCAGCATGTGGTATATGGTCCCTGGAACCTACCACAGTCCA
  GCTACTCCCACCTCACTCGCCAGGGTGAGACCCTCAATTTCCTGGAGATTGGATATTCCC
  GCTGCTGCCACTGCCGCAGCCACACAAACCGCCTAGAGTGTGCCAAACTTGTGTGGGAGG
  AAGCAATGAGCCGATTCTGTGAGGCCGAGTTCTCGGTCAAGACCCGACCCCACTGGTGCT
  GCACGCGGCAGGGGGAGGCTCGGTTCTCCTGCTTCCAGGAGGAAGCTCCCCAGCCACACT
  ACCAGCTCCGGGCCTGCCCCAGCCATCAGCCTGATATTTCCTCGGGTCTTGAGCTGCCTT
  TCCCTCCTGGGGTGCCCACATTGGACAATATCAAGAACATCTGCCACCTGAGGCGCTTCC
  GCTCTGTGCCACGCAACCTGCCAGCTACTGACCCCCTACAAAGGGAGCTGCTGGCACTGA
  TCCAGCTGGAGAGGGAGTTCCAGCGCTGCTGCCGCCAGGGGAACAATCACACCTGTACAT
  GGAAGGCCTGGGAGGATACCCTTGACAAATACTGTGACCGGGAGTATGCTGTGAAGACCC
  ACCACCACTTGTGTTGCCGCCACCCTCCCAGCCCTACTCGGGATGAGTGCTTTGCCCGTC
  GGGCTCCTTACCCCAACTATGACCGGGACATCTTGACCATTGACATCAGTCGAGTCACCC
  CCAACCTCATGGGCCACCTCTGTGGAAACCAAAGAGTTCTCACCAAGCATAAACATATTC
  CTGGGCTGATCCACAACATGACTGCCCGCTGCTGTGACCTGCCATTTCCAGAACAGGCCT
  GCTGTGCAGAGGAGGAGAAATTAACCTTCATCAATGATCTGTGTGGTCCCCGACGTAACA
  TCTGGCGAGACCCTGCCCTCTGCTGTTACCTGAGTCCTGGGGATGAACAGGTCAACTGCT
  TCAACATCAATTATCTGAGGAACGTGGCTCTAGTGTCTGGAGACACTGAGAACGCCAAGG
  GCCAGGGGGAGCAGGGCTCAACTGGAGGAACAAATATCAGCTCCACCTCTGAGCCCAAGG
  AAGAATGAGTCACCCCAGAGCCCTAGAGGGTCAGATGGGGGGAACCCCACCCTGCCCCAC
  CCATCTGAACACTCATTACACTAAACACCTCTTGGAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_004425
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004425.2, NP_004416.1
• RefSeq Size: 1837 bp
• RefSeq ORF: 1623 bp
• Locus ID: 1893
• Cytogenetics: 1q21.2
• Protein Families: Secreted Protein, Transmembrane
• Gene Summary: 'This gene encodes a soluble protein that is involved in endochondral bone formation, angiogenesis, and tumor biology. It also interacts with a variety of extracellular and structural proteins, contributing to the maintenance of skin integrity and homeostasis. Mutations in this gene are associated with lipoid proteinosis disorder (also known as hyalinosis cutis et mucosae or Urbach-Wiethe disease) that is characterized by generalized thickening of skin, mucosae and certain viscera. Alternatively spliced transcript variants encoding distinct isoforms have been described for this gene. [provided by RefSeq, Feb 2011]'
  Transcript Variant: This variant (1) represents the predominant transcript, and encodes isoform 1.