HPD (NM_002150) Human Untagged Clone

CAT#: SC319575

HPD (untagged)-Human 4-hydroxyphenylpyruvate dioxygenase (HPD), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HPD
• Synonyms: 4-HPPD; 4HPPD; GLOD3; HPPDASE; PPD
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002150.2 GGAGGTTTGACTAAGATCAATCATGACGACTTACAGTGACAAAGGGGCAAAGCCTGAGAG
  AGGCCGATTCCTCCACTTCCACTCTGTGACCTTCTGGGTTGGCAACGCCAAGCAGGCCGC
  GTCATTCTACTGCAGCAAGATGGGCTTTGAACCTCTAGCCTACAGGGGCCTGGAGACCGG
  TTCCCGGGAGGTGGTCAGCCATGTAATCAAACAAGGGAAGATTGTGTTTGTCCTCTCCTC
  AGCGCTCAACCCCTGGAACAAAGAGATGGGCGATCACCTGGTGAAACACGGTGACGGAGT
  GAAGGACATTGCGTTCGAGGTGGAAGATTGTGACTACATCGTGCAGAAAGCACGGGAACG
  GGGCGCCAAAATCATGCGGGAGCCCTGGGTAGAGCAAGACAAGTTTGGGAAGGTGAAGTT
  TGCTGTGCTGCAGACGTATGGGGACACCACACACACCCTGGTGGAGAAGATGAACTACAT
  CGGCCAATTCTTGCCTGGATATGAGGCCCCAGCGTTCATGGACCCCCTACTTCCTAAACT
  GCCCAAATGCAGTCTGGAGATGATCGACCACATTGTGGGAAACCAGCCTGATCAGGAGAT
  GGTGTCCGCCTCCGAATGGTACCTGAAAAACCTGCAGTTCCACCGCTTCTGGTCCGTGGA
  TGACACGCAGGTGCACACGGAATATAGCTCTCTGCGATCCATTGTGGTGGCCAACTATGA
  AGAGTCCATCAAGATGCCCATCAATGAGCCAGCGCCTGGCAAGAAGAAGTCCCAGATCCA
  GGAATATGTGGACTATAACGGGGGCGCTGGGGTCCAGCACATCGCTCTCAAGACCGAAGA
  CATCATCACAGCGATTCGCCACTTGAGAGAGAGAGGCCTGGAGTTCTTATCTGTTCCCTC
  CACGTACTACAAACAACTGCGGGAGAAGCTGAAGACGGCCAAGATCAAGGTGAAGGAGAA
  CATTGATGCCCTGGAGGAGCTGAAAATCCTGGTGGACTACGACGAGAAAGGCTACCTCCT
  GCAGATCTTCACCAAACCGGTGCAGGACCGGCCCACGCTCTTCCTGGAAGTCATCCAGCG
  CCACAACCACCAGGGTTTTGGAGCCGGCAACTTCAACTCACTGTTCAAGGCTTTCGAGGA
  GGAGCAGAACCTGCGGGGTAACCTCACCAACATGGAGACCAATGGGGTGGTGCCCGGCAT
  GTAAGCCCCGCCCACCCCACGGAGGCCACAGCCACACAGCCACGCCCCCTGATTCTGGAA
  CTCGCCCAACTTCCCTACTGGCTGCTCCCCTTGGGTCCCGCCCACCAGCGGACTCGGCCC
  CCAAGGCTCCGCCCACACTGACCACGCCCCTCGGCGGGGCCGCCCTCTGCTCCAGCCCTC
  CCGATTAAAGCGTGCCCCGGTCCAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_002150
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002150.2, NP_002141.1
• RefSeq Size: 1440 bp
• RefSeq ORF: 1182 bp
• Locus ID: 3242
• Cytogenetics: 12q24.31
• Domains: Glyoxalase
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Phenylalanine metabolism, Tyrosine metabolism, Ubiquinone and other terpenoid-quinone biosynthesis
• Gene Summary: 'The protein encoded by this gene is an enzyme in the catabolic pathway of tyrosine. The encoded protein catalyzes the conversion of 4-hydroxyphenylpyruvate to homogentisate. Defects in this gene are a cause of tyrosinemia type 3 (TYRO3) and hawkinsinuria (HAWK). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2010]'
  Transcript Variant: This variant (1) encodes the longer isoform (1).