Tbp7 (PSMC4) (NM_006503) Human Untagged Clone

CAT#: SC319607

PSMC4 (untagged)-Human proteasome (prosome, macropain) 26S subunit, ATPase, 4 (PSMC4), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PSMC4
• Synonyms: MIP224; RPT3; S6; TBP-7; TBP7
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_006503.2 CAGAGGCCGGCTTGGTCACTATGGAGGAGATAGGCATCTTGGTGGAGAAGGCTCAGGATG
  AGATCCCAGCACTGTCCGTGTCCCGGCCCCAGACCGGCCTGTCCTTCCTGGGCCCTGAGC
  CTGAGGACCTGGAGGACCTGTACAGCCGCTACAAGAAGCTGCAGCAAGAGCTGGAGTTCC
  TGGAGGTGCAGGAGGAATACATCAAAGATGAGCAAAAGAACCTGAAAAAGGAATTTCTCC
  ATGCCCAGGAGGAGGTGAAGCGAATCCAAAGCATCCCGCTGGTCATCGGACAATTTCTGG
  AGGCTGTGGATCAGAATACAGCCATCGTGGGCTCTACCACAGGCTCCAACTATTATGTGC
  GCATCCTGAGCACCATCGATCGGGAGCTGCTCAAGCCCAACGCCTCAGTGGCCCTCCACA
  AGCACAGCAATGCACTGGTGGACGTGCTGCCCCCCGAAGCCGACAGCAGCATCATGATGC
  TCACCTCAGACCAGAAGCCAGATGTGATGTACGCGGACATCGGAGGCATGGACATCCAGA
  AGCAGGAGGTGCGGGAGGCCGTGGAGCTCCCGCTCACGCATTTCGAGCTCTACAAGCAGA
  TCGGCATCGATCCCCCCCGAGGCGTCCTCATGTATGGCCCACCTGGCTGTGGGAAGACCA
  TGTTGGCAAAGGCGGTGGCACATCACACAACAGCTGCATTCATCCGGGTCGTGGGCTCGG
  AGTTTGTACAGAAGTATCTGGGTGAGGGCCCCCGCATGGTCCGGGATGTGTTCCGCCTGG
  CCAAGGAGAATGCACCTGCCATCATCTTCATAGACGAGATTGATGCCATCGCCACCAAGA
  GATTCGATGCTCAGACAGGGGCCGACAGGGAGGTTCAGAGGATCCTGCTGGAGCTGCTGA
  ATCAGATGGATGGATTTGATCAGAATGTCAATGTCAAGGTAATCATGGCCACAAACAGAG
  CAGACACCCTGGATCCGGCCCTGCTACGGCCAGGACGGCTGGACCGTAAAATTGAATTTC
  CACTTCCTGACCGCCGCCAGAAGAGATTGATTTTCTCCACTATCACTAGCAAGATGAACC
  TCTCTGAGGAGGTTGACTTGGAAGACTATGTGGCCCGGCCAGATAAGATTTCAGGAGCTG
  ATATTAACTCCATCTGTCAGGAGAGTGGAATGTTGGCTGTCCGTGAAAACCGCTACATTG
  TCCTGGCCAAGGACTTCGAGAAAGCATACAAGACTGTCATCAAGAAGGACGAGCAGGAGC
  ATGAGTTTTACAAGTGACCCTTCCCTTCCCTCCACCACACCACTCAGGGGCTGGGGCTTC
  TCTCGCACCCCCAGCACCTCTGTCCCAAAACCTCATTCCCTTTTTTCTTTACCCAGGATT
  GGTTTCTTCAATAAATAGATAAGATCGAATCCAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_006503
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006503.2, NP_006494.1
• RefSeq Size: 1450 bp
• RefSeq ORF: 1257 bp
• Locus ID: 5704
• Cytogenetics: 19q13.2
• Domains: AAA, AAA
• Protein Families: Druggable Genome
• Protein Pathways: Proteasome
• Gene Summary: 'The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a member of the triple-A family of ATPases that is a component of the 19S regulatory subunit and plays a role in 26S proteasome assembly. The encoded protein interacts with gankyrin, a liver oncoprotein, and may also play a role in Parkinson's disease through interactions with synphilin-1. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012]'
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.