ENO3 (NM_053013) Human Untagged Clone

CAT#: SC319614

ENO3 (untagged)-Human enolase 3 (beta, muscle) (ENO3), transcript variant 2


  "NM_053013" in other vectors (6)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol ENO3
Synonyms GSD13; MSE
Vector pCMV6-AC
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>OriGene sequence for NM_053013.1 CTGCCACCTAGACTCGGAGCTCCATCCAAACCTCCAGCGAAGACATCCCAGCCATGGCCA
TGCAGAAAATCTTTGCCCGGGAAATCTTGGACTCCAGGGGCAACCCCACGGTGGAGGTGG
ACCTGCACACGGCCAAGGGCCGATTCCGAGCAGCTGTGCCCAGTGGGGCTTCCACGGGTA
TCTATGAGGCTCTGGAACTAAGAGACGGAGACAAAGGCCGCTACCTGGGGAAAGGAGTCC
TGAAGGCTGTGGAGAACATCAACAGTACTCTGGGCCCTGCTCTGCTGCAAAAGAAACTAA
GCGTTGCGGATCAAGAAAAAGTTGACAAATTTATGATTGAGCTAGATGGGACCGAGAATA
AGTCCAAGTTTGGGGCCAATGCCATCCTGGGCGTGTCCTTGGCCGTGTGTAAGGCGGGAG
CAGCTGAGAAGGGGGTCCCCCTGTACCGCCACATCGCAGATCTCGCTGGGAACCCTGACC
TCATACTCCCAGTGCCAGCCTTCAATGTGATCAACGGGGGCTCCCATGCTGGAAACAAGC
TGGCCATGCAGGAGTTCATGATTCTGCCTGTGGGAGCCAGCTCCTTCAAGGAAGCCATGC
GCATTGGCGCCGAGGTCTACCACCACCTCAAGGGGGTCATCAAGGCCAAGTATGGGAAGG
ATGCCACCAATGTGGGTGATGAAGGTGGCTTCGCACCCAACATCCTGGAGAACAATGAGG
CCCTGGAGCTGCTGAAGACGGCCATCCAGGCGGCTGGTTACCCAGACAAGGTGGTGATCG
GCATGGATGTGGCAGCATCTGAGTTCTATCGCAATGGGAAGTACGATCTTGACTTCAAGT
CGCCTGATGATCCCGCACGGCACATCACTGGGGAGAAGCTCGGAGAGCTGTATAAGAGCT
TTATCAAGAACTATCCTGTGGTCTCCATCGAAGACCCCTTTGACCAGGATGACTGGGCCA
CTTGGACCTCCTTCCTCTCGGGGGTGAACATCCAGATTGTGGGGGATGACTTGACAGTCA
CCAACCCCAAGAGGATTGCCCAGGCCGTTGAGAAGAAGGCCTGCAACTGTCTGCTGCTGA
AGGTCAACCAGATCGGCTCGGTGACCGAATCGATCCAGGCGTGCAAACTGGCTCAGTCTA
ATGGCTGGGGGGTGATGGTGAGCCACCGCTCTGGGGAGACTGAGGACACATTCATTGCTG
ACCTTGTGGTGGGGCTCTGCACAGGACAGATCAAGACTGGCGCCCCCTGCCGCTCGGAGC
GTCTGGCCAAATACAACCAACTCATGAGGATCGAGGAGGCTCTTGGGGACAAGGCAATCT
TTGCTGGACGCAAGTTCCGTAACCCGAAGGCCAAGTGAGAAGCTGGAGGCTCCAGGACTC
CACTGGACAGACCCAGGTCTTCCAGACCTGCTTCCTGAAATAAACACTGGTGCCAACCAA
AAAAAAAAAAAAAAAAA
Restriction Sites Please inquire     
ACCN NM_053013
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_053013.1, NP_443739.1
RefSeq Size 1452 bp
RefSeq ORF 1305 bp
Locus ID 2027
Cytogenetics 17p13.2
Domains enolase
Protein Pathways Glycolysis / Gluconeogenesis, Metabolic pathways, RNA degradation
Gene Summary 'This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme is found in skeletal muscle cells in the adult where it may play a role in muscle development and regeneration. A switch from alpha enolase to beta enolase occurs in muscle tissue during development in rodents. Mutations in this gene have be associated glycogen storage disease. Alternatively spliced transcript variants encoding different isoforms have been described.[provided by RefSeq, Jul 2010]'
Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same protein (isoform 1).

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