RHOH (NM_004310) Human Untagged Clone

CAT#: SC319656

RHOH (untagged)-Human ras homolog gene family, member H (RHOH)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RHOH
• Synonyms: ARHH; TTF
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004310.2 AGCCCAACTGTTGTATTTTCAGTTCTTCCAGTGTGAATCAGTTAATATTCTCGGGAACGA
  GGGAGAGGTTGATCCTATGAGGAAATCAACCACAGTGAAAAGGCTTGGGCCGCTTTTGTT
  TTCACCTGCTTTTGTTGAACAAATTTGATTTCCGGAGTCAGTCATTTTACTGTCAAGACA
  TTTCTTCGGCATTCTGCAACAGTTTCCAACATGGCTAGATCCATCAGAAACTGAAGCCGT
  GGAGAACGCTCTCGGGGCCTTTGCCACTTCTTGGAGTAGAAGCCGACAGAGAGCTGTTTG
  GAAACTTCTCCTTCACACACCAGTTGAAGACTAGGCTTTGGAGGTTTTCAAAGCAGACGG
  TGCTTGGATGGGCAGGGAGAAGTAACATTCTGCAAATCGCCGTCAGAGGTCCTGAGGACA
  CAGACCTACCTGGCTTGCATTCCCCTTGCTGAATGGCGTGTGCTGCAGCTGCCCACTGAG
  GGCTCTTTTCCCTGGGATTCTGGACTTCAGAGTAGGACAGCAGGCTGGGAAGATGCTGAG
  TTCCATCAAGTGCGTGTTGGTGGGCGACTCTGCTGTGGGGAAAACCTCTCTGTTGGTGCG
  CTTCACCTCCGAGACCTTCCCGGAGGCCTACAAGCCCACAGTGTACGAGAACACAGGGGT
  GGACGTCTTCATGGATGGCATCCAGATCAGCCTGGGCCTCTGGGACACAGCCGGCAATGA
  CGCCTTCAGAAGCATCCGGCCCCTGTCCTACCAGCAGGCAGACGTGGTGCTGATGTGCTA
  CTCTGTGGCCAACCATAACTCATTCCTGAACTTGAAGAACAAGTGGATTGGTGAAATTAG
  GAGCAACTTGCCCTGTACCCCTGTGCTGGTGGTGGCCACCCAGACTGACCAGCGGGAGAT
  GGGGCCCCACAGGGCCTCCTGCGTCAATGCCATGGAAGGGAAGAAACTGGCCCAGGATGT
  CAGAGCCAAGGGCTACCTGGAGTGCTCAGCCCTTAGCAATCGGGGAGTACAGCAGGTGTT
  TGAGTGCGCCGTCCGAACTGCCGTCAACCAGGCCAGGAGACGAAACAGAAGGAGGCTCTT
  CTCCATCAATGAGTGCAAGATCTTCTAAACCCCAAGAGACTTCACACAACACTTATGTAT
  GCACCCCAAAGACTAATGGGGAGAGGGAGGGCCGGGAAGCCAGGAAAGCTTGGTGTTTTC
  TCTGGGTACACCCCAAGCAGCGTCTCCTTTTGGATACAGTTATTGATGAGGCTTGGCCAC
  TGGATGTTTTCACTAACTACACTCTACAAGTGAACTCCTTGCCCAGGCCAGTTAGAAAAT
  CCCTTGGGGAACTGTGATGAATATTCCATCTTTGATTAAAAAAGTGAAATAGTCTCCATA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_004310
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004310.2, NP_004301.1
• RefSeq Size: 1516 bp
• RefSeq ORF: 576 bp
• Locus ID: 399
• Cytogenetics: 4p14
• Domains: ras, RAS, RHO, RAB
• Protein Families: Transcription Factors
• Protein Pathways: Leukocyte transendothelial migration
• Gene Summary: 'The protein encoded by this gene is a member of the Ras superfamily of guanosine triphosphate (GTP)-metabolizing enzymes. The encoded protein is expressed in hematopoietic cells, where it functions as a negative regulator of cell growth and survival. This gene may be hypermutated or misexpressed in leukemias and lymphomas. Chromosomal translocations in non-Hodgkin's lymphoma occur between this locus and B-cell CLL/lymphoma 6 (BCL6) on chromosome 3, leading to the production of fusion transcripts. Alternative splicing in the 5' untranslated region results in multiple transcript variants that encode the same protein. [provided by RefSeq, May 2013]'
  Transcript Variant: This variant (6) lacks an exon in the 5' UTR, compared to variant 1. Variants 1 through 12 encode the same protein.