Glutathione S Transferase theta 1 (GSTT1) (NM_000853) Human Untagged Clone

CAT#: SC319671

GSTT1 (untagged)-Human glutathione S-transferase theta 1 (GSTT1)


  "NM_000853" in other vectors (4)

Reconstitution Protocol

USD 310.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol GSTT1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_000853.1 GACTCCCTCTGGTTTCCGGTCAGGTCGGTCGGTCCCCACTATGGGCCTGGAGCTGTACCT
GGACCTGCTGTCCCAGCCCTGCCGCGCTGTTTACATCTTTGCCAAGAAGAACGACATTCC
CTTCGAGCTGCGCATCGTGGATCTGATTAAAGGTCAGCACTTAAGCGATGCCTGTGCCCA
GGTGAACCCCCTCAAGAAGGTGCCAGCCTTGAAGGACGGGGACTTCACCTTGACGGAGAG
TGTGGCCATCCTGCTCTACCTGACGCGCAAATATAAGGTCCCTGACTACTGGTACCCTCA
GGACCTGCAGGCCCGTGCCCGTGTGGATGAGTACCTGGCATGGCAGCACACGACTCTGCG
GAGAAGCTGCCTCCGGGCCTTGTGGCATAAGGTGATGTTCCCTGTTTTCCTGGGTGAGCC
AGTATCTCCCCAGACACTGGCAGCCACCCTGGCAGAGTTGGATGTGACCCTGCAGTTGCT
CGAGGACAAGTTCCTCCAGAACAAGGCCTTCCTTACTGGTCCTCACATCTCCTTAGCTGA
CCTCGTAGCCATCACGGAGCTGATGCATCCCGTGGGTGCTGGCTGCCAAGTCTTCGAAGG
CCGACCCAAGCTGGCCACATGGCGGCAGCGCGTGGAGGCAGCAGTGGGGGAGGACCTCTT
CCAGGAGGCCCATGAGGTCATTCTGAAGGCCAAGGACTTCCCACCTGCAGACCCCACCAT
AAAACAGAAGCTGATGCCCTGGGTGCTGGCCATGATCCGGTGAGCTGGGAAGCCTCACCC
TTGCACCGTCCTCAGCAGTCCACAAAGCATTTTCATTTCTAATGGCCCATGGGAGCCAGG
CCCAGAAAGCAGGAATGGCTTGCTTAAGACTTGCCCAAGTCCCAGAGCACCTCACCTCCC
GAAGCCACCATCCCCACCCTGTCTTCCACAGCCGCCTGAAAGCCACAATGAGAATGATGC
ACACTGAGGCCTTGTGTCCTTTAATCACTGCATTTCATTTTGATTTTGGATAATAAACCT
GGGCTCAGCCTGAGCCTCTGCTTCGAAAAAAAAAAAAAAAAAAAAAAAAAAA
Restriction Sites Please inquire     
ACCN NM_000853
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000853.1, NP_000844.1
RefSeq Size 1004 bp
RefSeq ORF 723 bp
Locus ID 2952
Cytogenetics 22q11.23
Domains GST_N, GST_C
Protein Pathways Drug metabolism - cytochrome P450, Glutathione metabolism, Metabolism of xenobiotics by cytochrome P450
Gene Summary 'The protein encoded by this gene, glutathione S-transferase (GST) theta 1 (GSTT1), is a member of a superfamily of proteins that catalyze the conjugation of reduced glutathione to a variety of electrophilic and hydrophobic compounds. Human GSTs can be divided into five main classes: alpha, mu, pi, theta, and zeta. The theta class includes GSTT1, GSTT2, and GSTT2B. GSTT1 and GSTT2/GSTT2B share 55% amino acid sequence identity and may play a role in human carcinogenesis. The GSTT1 gene is haplotype-specific and is absent from 38% of the population. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2015]'
Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).

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