TAZ (WWTR1) (NM_015472) Human Untagged Clone

CAT#: SC319897

WWTR1 (untagged)-Human WW domain containing transcription regulator 1 (WWTR1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: WWTR1
• Synonyms: TAZ
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_015472.3 GCTTCTCCTGCGCCTCCTTTTCGCCCAGCACTAGCGCCTTAGGCCAGCTCGGGGGATGTG
  AGAGCCGAAGCCCTTAGACTGCCAGGCACAGAGTCGGGTCGGGATTTGTCAGCCAAGCCT
  CGGCTCCAGCTCCGCAATCTCGGGACTCACCCGAGCGACCCAGGCCCGACGGCAAGTTCG
  GGCGGGACGGCGGCCGCCGCGCGCTCAGGCTCAGCTTCGCTGCCCGCCCAGAAGATGAAT
  CCGGCCTCGGCGCCCCCTCCGCTCCCGCCGCCTGGGCAGCAAGTGATCCACGTCACGCAG
  GACCTAGACACAGACCTCGAAGCCCTCTTCAACTCTGTCATGAATCCGAAGCCTAGCTCG
  TGGCGGAAGAAGATCCTGCCGGAGTCTTTCTTTAAGGAGCCTGATTCGGGCTCGCACTCG
  CGCCAGTCCAGCACCGACTCGTCGGGCGGCCACCCGGGGCCTCGACTGGCTGGGGGTGCC
  CAGCATGTCCGCTCGCACTCGTCGCCCGCGTCCCTGCAGCTGGGCACCGGCGCGGGTGCT
  GCGGGTAGCCCCGCGCAGCAGCACGCGCACCTCCGCCAGCAGTCCTACGACGTGACCGAC
  GAGCTGCCACTGCCCCCGGGCTGGGAGATGACCTTCACGGCCACTGGCCAGAGGTACTTC
  CTCAATCACATAGAAAAAATCACCACATGGCAAGACCCTAGGAAGGCGATGAATCAGCCT
  CTGAATCATATGAACCTCCACCCTGCCGTCAGTTCCACACCAGTGCCTCAGAGGTCCATG
  GCAGTATCCCAGCCAAATCTCGTGATGAATCACCAACACCAGCAGCAGATGGCCCCCAGT
  ACCCTGAGCCAGCAGAACCACCCCACTCAGAACCCACCCGCAGGGCTCATGAGTATGCCC
  AATGCGCTGACCACTCAGCAGCAGCAGCAGCAGAAACTGCGGCTTCAGAGAATCCAGATG
  GAGAGAGAAAGGATTCGAATGCGCCAAGAGGAGCTCATGAGGCAGGAAGCTGCCCTCTGT
  CGACAGCTCCCCATGGAAGCTGAGACTCTTGCCCCAGTTCAGGCTGCTGTCAACCCACCC
  ACGATGACCCCAGACATGAGATCCATCACTAATAATAGCTCAGATCCTTTCCTCAATGGA
  GGGCCATATCATTCGAGGGAGCAGAGCACTGACAGTGGCCTGGGGTTAGGGTGCTACAGT
  GTCCCCACAACTCCGGAGGACTTCCTCAGCAATGTGGATGAGATGGATACAGGAGAAAAC
  GCAGGACAAACACCCATGAACATCAATCCCCAACAGACCCGTTTCCCTGATTTCCTTGAC
  TGTCTTCCAGGAACAAACGTTGACTTAGGAACTTTGGAATCTGAAGACCTGATCCCCCTC
  TTCAATGATGTAGAGTCTGCTCTGAACAAAAGTGAGCCCTTTCTAACCTGGCTGTAATCA
  CTACCATTGTAACTTGGATGTAGCCATGACCTTACATTTCCTGGGCCTCTTGGAAAAAGT
  GATGGAGCAGAGCAAGTCTGCAGGTGCACCACTTCCCGCCTCCATGACTCGTGCTCCCTC
  CTTTTTATGTTGCCAGTTTAATCATTGCCTGGTTTTGATTGAGAGTAACTTAAGTTAAAC
  ATAAATAAATATTCTATTTTCATTTTCTGCAAGCCTGCGTTCTTGTGACAGATTATACAG
  AATTGTGTCTGCAGGATTGATTATGCAGAATACTTTTCTCTTTCTTCTCTGCTGCCCCAT
  GGCTAAGCTTTATGGGTGTTAATTGAAATTTATACACCAATTGATTTTAAACCATAAAAA
  GCTGACCACAGGCAGTTACTTCTGAGGGCATCTTGGTCCAGGAAATGTGCACAAAATTCG
  ACCTGATTTACAGTTTCAAAAACTGTATTGATGACAGTAGTACCAAATGCTTTAAAAACT
  ATTTAACTTGAGCTTTAAAAATCATTGTATGGATAGTAAAATTCTACTGTATGGAATACA
  ATGTAATTTTGAATCCAAAAAAAAAAAAAAAAAAA
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015472.3 unedited
  TGCTTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGGCACGAGGGCTTCTCCTGCGC
  CTCCTTTTCGCCCAGCACTAGCGCCTTAGGCCAGCTCGGGGGATGTGAGAGCCGAAGCCC
  TTAGACTGCCAGGCACAGAGTCGGGTCGGGATTTGTCAGCCAAGCCTCGGCTCCAGCTCC
  GCAATCTCGGGACTCACCCGAGCGACCCAGGCCCGACGGCAAGTTCGGGCGGGACGGCGG
  CCGCCGCGCGCTCAGGCTCAGCTTCGCTGCCCGCCCAGAAGATGAATCCGGCCTCGGCGC
  CCCCTCCGCTCCCGCCGCCTGGGCAGCAAGTGATCCACGTCACGCAGGACCTAGACACAG
  ACCTCGAAGCCCTCTTCAACTCTGTCATGAATCCGAAGCCTAGCTCGTGGCGGAAGAAGA
  TCCTGCCGGAGTCTTTCTTTAAGGAGCCTGATTCGGGCTCGCACTCGCGCCAGTCCAGCA
  CCGACTCGTCGGGCGGCCACCCGGGGCCTCGACTGGCTGGGGGTGCCCAGCATGTCCGCT
  CGCACTCGTCGCCCGCGTCCCTGCAGCTGGGCACCGGCGCGGGTGCTGCGGGTAGCCCCG
  CGCAGCAGCACGCGCACCTCCGCCAGCAGTCCTACGACGTGACCGACGAGCTGCCACTGC
  CCCCGGGCTGGGAGATGACCTTCACGGCCACTGGCCAGAGGTACTTCCTCAATCACATAG
  AAAAAATCACCACATGGCAAGACCCTAGAAGGCGATGAATCAGCCTCTGAATCATATGAA
  CCTCCACCCTGCCGTCAGTTCCACACCAGTGCCTCAGAGTCCATGCAGTTATCCCAGCCA
  AATCTCGTGATGATCACCACACCAGCAGCAGATGCCCCAGTACCCTGAGCCAGCAGACCC
  CCCCACTCAGACCACCCGCAGGCTCATGATATGCCCATGGCCTGACATCACAGCAGCAGC
  AGCAAACTGCGCTTCGAGAATCAGAATGGGAGAGAGAAAGATTCGAATGCCCC
• Restriction Sites: Please inquire
• ACCN: NM_015472
• ORF Size: 1203 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_015472.3, NP_056287.1
• RefSeq Size: 2015
• RefSeq ORF: 1203
• Locus ID: 25937
• Domains: WW
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: Transcriptional coactivator which acts as a downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. WWTR1 enhances PAX8 and NKX2-1/TTF1-dependent gene activation. Regulates the nuclear accumulation of SMADS and has a key role in coupling them to the transcriptional machinery such as the mediator complex. Regulates embryonic stem-cell self-renewal, promotes cell proliferation and epithelial-mesenchymal transition. [UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant 12) differs in the 5' UTR compared to variant 4. Variants 1 through 4 all encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.