Seladin 1 (DHCR24) (NM_014762) Human Untagged Clone

CAT#: SC320135

DHCR24 (untagged)-Human 24-dehydrocholesterol reductase (DHCR24)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DHCR24
• Synonyms: DCE; Nbla03646; seladin-1; SELADIN1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_014762.3 GCGGAGCTGGCGGCAGTGACAGGAGGCGCGAACCCGCAGCGCTTACCGCGCGGCGCCGCA
  CCATGGAGCCCGCCGTGTCGCTGGCCGTGTGCGCGCTGCTCTTCCTGCTGTGGGTGCGCC
  TGAAGGGGCTGGAGTTCGTGCTCATCCACCAGCGCTGGGTGTTCGTGTGCCTCTTCCTCC
  TGCCGCTCTCGCTTATCTTCGATATCTACTACTACGTGCGCGCCTGGGTGGTGTTCAAGC
  TCAGCAGCGCTCCGCGCCTGCACGAGCAGCGCGTGCGGGACATCCAGAAGCAGGTGCGGG
  AATGGAAGGAGCAGGGTAGCAAGACCTTCATGTGCACGGGGCGCCCTGGCTGGCTCACTG
  TCTCACTACGTGTCGGGAAGTACAAGAAGACACACAAAAACATCATGATCAACCTGATGG
  ACATTCTGGAAGTGGACACCAAGAAACAGATTGTCCGTGTGGAGCCCTTGGTGACCATGG
  GCCAGGTGACTGCCCTGCTGACCTCCATTGGCTGGACTCTCCCCGTGTTGCCTGAGCTTG
  ATGACCTCACAGTGGGGGGCTTGATCATGGGCACAGGCATCGAGTCATCATCCCACAAGT
  ACGGCCTGTTCCAACACATCTGCACTGCTTACGAGCTGGTCCTGGCTGATGGCAGCTTTG
  TGCGATGCACTCCGTCCGAAAACTCAGACCTGTTCTATGCCGTACCCTGGTCCTGTGGGA
  CGCTGGGTTTCCTGGTGGCCGCTGAGATCCGCATCATCCCTGCCAAGAAGTACGTCAAGC
  TGCGTTTCGAGCCAGTGCGGGGCCTGGAGGCTATCTGTGCCAAGTTCACCCACGAGTCCC
  AGCGGCAGGAGAACCACTTCGTGGAAGGGCTGCTCTACTCCCTGGATGAGGCTGTCATTA
  TGACAGGGGTCATGACAGATGAGGCAGAGCCCAGCAAGCTGAATAGCATTGGCAATTACT
  ACAAGCCGTGGTTCTTTAAGCATGTGGAGAACTATCTGAAGACAAACCGAGAGGGCCTGG
  AGTACATTCCCTTGAGACACTACTACCACCGCCACACGCGCAGCATCTTCTGGGAGCTCC
  AGGACATCATCCCCTTTGGCAACAACCCCATCTTCCGCTACCTCTTTGGCTGGATGGTGC
  CTCCCAAGATCTCCCTCCTGAAGCTGACCCAGGGTGAGACCCTGCGCAAGCTGTACGAGC
  AGCACCACGTGGTGCAGGACATGCTGGTGCCCATGAAGTGCCTGCAGCAGGCCCTGCACA
  CCTTCCAAAACGACATCCACGTCTACCCCATCTGGCTGTGTCCGTTCATCCTGCCCAGCC
  AGCCAGGCCTAGTGCACCCCAAAGGAAATGAGGCAGAGCTCTACATCGACATTGGAGCAT
  ATGGGGAGCCGCGTGTGAAACACTTTGAAGCCAGGTCCTGCATGAGGCAGCTGGAGAAGT
  TTGTCCGCAGCGTGCATGGCTTCCAGATGCTGTATGCCGACTGCTACATGAACCGGGAGG
  AGTTCTGGGAGATGTTTGATGGCTCCTTGTACCACAAGCTGCGAGAGAAGCTGGGTTGCC
  AGGACGCCTTCCCCGAGGTGTACGACAAGATCTGCAAGGCCGCCAGGCACTGAGCTGGAG
  CCCGCCTGGAGAGACAGACACGTGTGAGTGGTCAGGCATCTTCCCTTCACTCAAGCTTGG
  CTGCTTTCCTAGATCCACACTTTCAAAGAGAAACCCCTCCAGAACTCCCACCCTGACAGC
  CCAACACCACCTTCCTCCTGGCTTCCAGGGGGCAGCCCAGTGGAATGGAAAGAATGTGGG
  ATTTGGAGTCAGACAAGCCTGAGTCCAGTTCCCCGTTTAGAACTCATTAGCTGTGTGACT
  CTGGGTGAGTCCCTTAACCCCTCTGAGCCCGGGTCTCTTCATTAGTTGAAAGGGATAGTA
  ATACCTACTTGCAGGTTGTTGTCATCTGAGTTGAGCACTGGTCACATTGAAGGTGCTGGG
  TAAGTGGTAGCTCTTGTTGCTTCCCGTTCAGCGTCACATCTGCAGTGGAGCCTGAAAAGG
  CTCCACATTAGGTCACCTGTGCACAGCCATGGCTGGAATGATGAAGGGGATACGCTGGAG
  TTGCCCTGCCATCGCCTCCATCAGCCAGACGAGGTCCTCACAGGAGAAGGACAGCTCTTC
  CCCACCCTGGGATCTCAGGAGGGCAGCCACGGAGTGGGGAGGCCCCAGATGCGCTGTGCC
  AAAGCCAGGTCCGAGGCCAAAGTTCTCCCTGCCATCCTTGGTGCCGTCCTGCCCCTTCCT
  CCTTCATGCCTGGGCCTGCAGGCCCACCCCAGCCACCACTGAGTCCACTCGGAGTGCCCT
  GTGTTCCTGGAGAAGGCATTCCAGGGTTGAATCTTGTCCCAGCCTCAGCCTGGGACACCT
  AGGTGGAGAGAGTGGTCTCCGCTCTGAATTGGATCCAGGGGACCTGGGCTCATTCTTCTT
  GGCTCACCAACCCTGCAGGCCTCATCTTTCCCAAAACCCACTTTGTCTTGGTGGGAGTGG
  GTCCGCGCTGCTCTGCAGCAGGGGCTGGGGAGTGGGCAGCATCAGGTGGGAAAGTGGAGT
  CCACCCTCATGTTTCTGTAGGATTCTCACCGTGGGGCTGGAAGAAAAGAGCATCGACTTG
  ATTTCTCCAACCACTCATCCCTCTTTTTCTTTCTTCCACCACTCCCCACCCCAGCTGTAG
  TTAATTTCAGTGCCTTACAAATCCTAAGCTCAGAGAAAGTTCCATTTCCGTTCCAGAGGG
  AAGGGAACCTCCCTAGGTCCTTCCCTGGCTTGTTATAACGCAAAGCTTGGTTGTTTATGC
  AACTCTATCTTAAGAACTGCCCAGCCTCAGCTGAAAACCCGAATCTGAGAAGGAATTGCG
  TCATGTAAGGGAAGCTGGAATTAAGGGAGCTGAGCCAGTCATGGTTGTGGCGTGTGAGTC
  AGGAGACCTAGGTTTCAGCCCCTCTCTACTGTCAGCGAGCTGTGCAACGTGGGCAAGTCA
  TTGTCCTCTGAGCTGCAGTTTCCTCATCTGTCACATCGCTACAGACAAGACCTCCCTGGA
  ACCCTTCTGATTGTCTTAGACACTGTGGTTGCAAAACCCACGGAAAGCCTCATTTGTGTG
  GAAAGTCAGAGGAAAAATGATCCAGTGGACACTTGGGGATTATCTGTCATTCAAGATCCT
  TCCTTCAACCCCAAGGTCAGCTCCCATCTCATTTCCAGAAAGGCTCATACCTGGCTTGCA
  GGGAAGCATCTGTCTTGTCATTCCAGGTGCCAGAATCCTCTCAGAGTCATTGAAGGGTGT
  TCACCCATCCCACCCAAGGCTTGGCACACTGCCAGTGTCTTAGCAGGGTCTTGTGAGGGC
  TGGGGGCATCCAGGCACTCAGAAGGCAAAGGAACCACCCTACCCATTTGGCCTCTGGAGG
  GGGCAGAAGAAAGAAAGAAACCTCATCCTATATTTTACAAAGCATGTGAATTCTGGCATT
  AGCTCTCATAGGAGACCCATGTGCTTCCTTGCTCAGTGCAAAACTGATGATTCTACTTGC
  TGTAGATGAATGGTTAACACGAGCTAGTTAAACAGTGCCATTGTTTTGCCAGTGAAGCCT
  CCAACCCTAAGCCACTGGGACGGTGGCCAGAGATGCCAGCAGCCTCTGTCGCCCTTAGTC
  ATATAACCAAAATCCAGACCTTATCCACAACCCGGGGCTTGGAAAGGAAGGTATTTTGGA
  ATCACACCCTCCGGTTATGTTGCTCCAGTAAAATCTTGCCTGGAAAGAGGCAGTCTTCTT
  AGCATGGTGAGCTGAGTTCATGGCTTTTTTTTGTAGCCAGTCCTGTCCCTGGCCATCCAT
  GTGATGGTTTTGGATGGAGTTAAACTTGATGCCAGTGGGCAGTGCATGTGGAAAGTATCA
  GAGTAAGGCTCTCCCCTCCAGAGCCCTGAGTTTCTTGGCTGCATGAAGGTTTTCTTTAGA
  ATCAGAATTGTAGCCAGTTTCTTTGGCCAGAAGGATGAATACTTGGATATTACTGAAAGG
  GAGGGGTGGAGATGGGTGTGGCAGTGTATGGTGTGTGATTTTTATTTTCTTCTTTGGTCA
  TGGGGGCCAAGGAGAAAGGCATGAATCTTCCCTGTCAGGCTCTTACAGCCACAGGCACTG
  TGTCTACTGTCTGGAAGACATGTCCCCGTGGCTGTGGGGCCGCTGCTTCTGTTTAAATAA
  AAGTGGCCTGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_014762
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014762.3, NP_055577.1
• RefSeq Size: 4286 bp
• RefSeq ORF: 1551 bp
• Locus ID: 1718
• Cytogenetics: 1p32.3
• Domains: FAD_binding_4
• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transmembrane
• Protein Pathways: Metabolic pathways, Steroid biosynthesis
• Gene Summary: 'This gene encodes a flavin adenine dinucleotide (FAD)-dependent oxidoreductase which catalyzes the reduction of the delta-24 double bond of sterol intermediates during cholesterol biosynthesis. The protein contains a leader sequence that directs it to the endoplasmic reticulum membrane. Missense mutations in this gene have been associated with desmosterolosis. Also, reduced expression of the gene occurs in the temporal cortex of Alzheimer disease patients and overexpression has been observed in adrenal gland cancer cells. [provided by RefSeq, Jul 2008]'