MGAT2 (NM_001015883) Human Untagged Clone

CAT#: SC320161

MGAT2 (untagged)-Human mannosyl (alpha-1,6-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase (MGAT2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MGAT2
• Synonyms: GNT2, CDGS2, GNT-II, GLCNACTII
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001015883.1 CGGGGCAGTTGCGGGTTGTCATAACGGTCCCCGCCGGAGTGAGGCGAGGCCGCGTCGCTC
  AGTTCTGGCCGTCTAGGGCCCCTGTAAGGATGAGAGCGCAGAGGACGCAGGGCCGCTGGA
  GGCGCAGGTAACGAAGCTAGGGTGCGGTTGGGACCGCGGCTGAGCTTTTTCCGGGACCCG
  TGGTGCTGAATGGAGAGGACGGAGACGAAGCCGAGCCGCGGCTCCTAGCGGCGGCGCCGA
  CGCTCGAGCTGTAGCTGCCAGGCGAGGATGTGTGGAGCGCAGGCGGCGCGGGGTAAATGA
  GAGGTCTCGGGCCCCAGGACCCCCGGGGCCCGGGATGAGTTAGCGAGGGCAGCCGCGGGG
  GCCAGTTCCGACCGTGACAGGCCAAGGCGACGGCCGCCGCCCGCCCGCCCCTTCCGTGCA
  GAAGCAGCTGCTCCTTTCCGCGCCCGCCCGCCTGCGCTCCCGGCCCTGGAGACCATGAGG
  TTCCGCATCTACAAACGGAAGGTGCTAATCCTGACGCTCGTGGTGGCCGCCTGCGGCTTC
  GTCCTCTGGAGCAGCAATGGGCGACAAAGGAAGAACGAGGCCCTCGCCCCACCGTTGCTG
  GACGCCGAACCCGCGCGGGGTGCCGGCGGCCGCGGTGGGGACCACCCCTCTGTGGCTGTG
  GGCATCCGCAGGGTCTCCAACGTGTCGGCGGCTTCCCTGGTCCCGGCGGTCCCCCAGCCC
  GAGGCGGACAACCTGACGCTGCGGTACCGGTCCCTGGTGTACCAGCTGAACTTTGATCAG
  ACCCTGAGGAATGTAGATAAGGCTGGCACCTGGGCCCCCCGGGAGCTGGTGCTGGTGGTC
  CAGGTGCATAACCGGCCCGAATACCTCAGACTGCTGCTGGACTCACTTCGAAAAGCCCAG
  GGAATTGACAACGTCCTCGTCATCTTTAGCCATGACTTCTGGTCGACCGAGATCAATCAG
  CTGATCGCCGGGGTGAATTTCTGTCCGGTTCTGCAGGTGTTCTTTCCTTTCAGCATTCAG
  TTGTACCCTAACGAGTTTCCAGGTAGTGACCCTAGAGATTGTCCCAGAGACCTGCCGAAG
  AATGCCGCTTTGAAATTGGGGTGCATCAATGCTGAGTATCCCGACTCCTTCGGCCATTAT
  AGAGAGGCCAAATTCTCCCAGACCAAACATCACTGGTGGTGGAAGCTGCATTTTGTGTGG
  GAAAGAGTGAAAATTCTTCGAGATTATGCTGGCCTTATACTTTTCCTAGAAGAGGATCAC
  TACTTAGCCCCAGACTTTTACCATGTCTTCAAAAAGATGTGGAAACTGAAGCAGCAAGAG
  TGCCCTGAATGTGATGTTCTCTCCCTGGGGACCTATAGTGCCAGTCGCAGTTTCTATGGC
  ATGGCTGACAAGGTAGATGTGAAAACTTGGAAATCCACAGAGCACAATATGGGTCTAGCC
  TTGACCCGGAATGCCTATCAGAAGCTGATCGAGTGCACAGACACTTTCTGTACTTATGAT
  GATTATAACTGGGACTGGACTCTTCAATACTTGACTGTATCTTGTCTTCCAAAATTCTGG
  AAAGTGCTGGTTCCTCAAATTCCTAGGATCTTTCATGCTGGAGACTGTGGTATGCATCAC
  AAGAAAACCTGTAGACCATCCACTCAGAGTGCCCAAATTGAGTCACTCTTAAATAATAAC
  AAACAATACATGTTTCCAGAAACTCTAACTATCAGTGAAAAGTTTACTGTGGTAGCCATT
  TCCCCACCTAGAAAAAATGGAGGGTGGGGAGATATTAGGGACCATGAACTCTGTAAAAGT
  TATAGAAGACTGCAGTGAAAATCACAGTTACAAAAGCGACAGTCTTCTATTTTTGATATT
  TGTCCAAACAGGACATACAATTGAATAAAAGAGTTTAGGAACTGGAAAAAAAAAAAAAAA
  AAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_001015883
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001015883.1, NP_001015883.1
• RefSeq Size: 2531 bp
• RefSeq ORF: 1344 bp
• Locus ID: 4247
• Cytogenetics: 14q21.3
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis
• Gene Summary: 'The product of this gene is a Golgi enzyme catalyzing an essential step in the conversion of oligomannose to complex N-glycans. The enzyme has the typical glycosyltransferase domains: a short N-terminal cytoplasmic domain, a hydrophobic non-cleavable signal-anchor domain, and a C-terminal catalytic domain. Mutations in this gene may lead to carbohydrate-deficient glycoprotein syndrome, type II. The coding region of this gene is intronless. Transcript variants with a spliced 5' UTR may exist, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Both variants 1 and 2 encode the same protein.