CD18 (ITGB2) (NM_000211) Human Untagged Clone

CAT#: SC320165

ITGB2 (untagged)-Human integrin, beta 2 (complement component 3 receptor 3 and 4 subunit) (ITGB2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ITGB2
• Synonyms: CD18; LAD; LCAMB; LFA-1; MAC-1; MF17; MFI7
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000211.2 GTTGGGCCTGAGACCGTCACCAAGACCCCTTCCCTCCACAGGACATGCTGGGCCTGCGCC
  CCCCACTTCTCGCCCTGGTGGGGCTGCTCTCCCTCGGGTGCGTCCTCTCTCAGGAGTGCA
  CGAAGTTCAAGGTCAGCAGCTGCCGGGAATGCATCGAGTCGGGGCCCGGCTGCACCTGGT
  GCCAGAAGCTGAACTTCACAGGGCCGGGGGATCCTGACTCCATTCGCTGCGACACCCGGC
  CACAGCTGCTCATGAGGGGCTGTGCGGCTGACGACATCATGGACCCCACAAGCCTCGCTG
  AAACCCAGGAAGACCACAATGGGGGCCAGAAGCAGCTGTCCCCACAAAAAGTGACGCTTT
  ACCTGCGACCAGGCCAGGCAGCAGCGTTCAACGTGACCTTCCGGCGGGCCAAGGGCTACC
  CCATCGACCTGTACTATCTGATGGACCTCTCCTACTCCATGCTTGATGACCTCAGGAATG
  TCAAGAAGCTAGGTGGCGACCTGCTCCGGGCCCTCAACGAGATCACCGAGTCCGGCCGCA
  TTGGCTTCGGGTCCTTCGTGGACAAGACCGTGCTGCCGTTCGTGAACACGCACCCTGATA
  AGCTGCGAAACCCATGCCCCAACAAGGAGAAAGAGTGCCAGCCCCCGTTTGCCTTCAGGC
  ACGTGCTGAAGCTGACCAACAACTCCAACCAGTTTCAGACCGAGGTCGGGAAGCAGCTGA
  TTTCCGGAAACCTGGATGCACCCGAGGGTGGGCTGGACGCCATGATGCAGGTCGCCGCCT
  GCCCGGAGGAAATCGGCTGGCGCAACGTCACGCGGCTGCTGGTGTTTGCCACTGATGACG
  GCTTCCATTTCGCGGGCGACGGGAAGCTGGGCGCCATCCTGACCCCCAACGACGGCCGCT
  GTCACCTGGAGGACAACTTGTACAAGAGGAGCAACGAATTCGACTACCCATCGGTGGGCC
  AGCTGGCGCACAAGCTGGCTGAAAACAACATCCAGCCCATCTTCGCGGTGACCAGTAGGA
  TGGTGAAGACCTACGAGAAACTCACCGAGATCATCCCCAAGTCAGCCGTGGGGGAGCTGT
  CTGAGGACTCCAGCAATGTGGTCCATCTCATTAAGAATGCTTACAATAAACTCTCCTCCA
  GGGTATTCCTGGATCACAACGCCCTCCCCGACACCCTGAAAGTCACCTACGACTCCTTCT
  GCAGCAATGGAGTGACGCACAGGAACCAGCCCAGAGGTGACTGTGATGGCGTGCAGATCA
  ATGTCCCGATCACCTTCCAGGTGAAGGTCACGGCCACAGAGTGCATCCAGGAGCAGTCGT
  TTGTCATCCGGGCGCTGGGCTTCACGGACATAGTGACCGTGCAGGTCCTTCCCCAGTGTG
  AGTGCCGGTGCCGGGACCAGAGCAGAGACCGCAGCCTCTGCCATGGCAAGGGCTTCTTGG
  AGTGCGGCATCTGCAGGTGTGACACTGGCTACATTGGGAAAAACTGTGAGTGCCAGACAC
  AGGGCCGGAGCAGCCAGGAGCTGGAAGGAAGCTGCCGGAAGGACAACAACTCCATCATCT
  GCTCAGGGCTGGGGGACTGTGTCTGCGGGCAGTGCCTGTGCCACACCAGCGACGTCCCCG
  GCAAGCTGATATACGGGCAGTACTGCGAGTGTGACACCATCAACTGTGAGCGCTACAACG
  GCCAGGTCTGCGGCGGCCCGGGGAGGGGGCTCTGCTTCTGCGGGAAGTGCCGCTGCCACC
  CGGGCTTTGAGGGCTCAGCGTGCCAGTGCGAGAGGACCACTGAGGGCTGCCTGAACCCGC
  GGCGTGTTGAGTGTAGTGGTCGTGGCCGGTGCCGCTGCAACGTATGCGAGTGCCATTCAG
  GCTACCAGCTGCCTCTGTGCCAGGAGTGCCCCGGCTGCCCCTCACCCTGTGGCAAGTACA
  TCTCCTGCGCCGAGTGCCTGAAGTTCGAAAAGGGCCCCTTTGGGAAGAACTGCAGCGCGG
  CGTGTCCGGGCCTGCAGCTGTCGAACAACCCCGTGAAGGGCAGGACCTGCAAGGAGAGGG
  ACTCAGAGGGCTGCTGGGTGGCCTACACGCTGGAGCAGCAGGACGGGATGGACCGCTACC
  TCATCTATGTGGATGAGAGCCGAGAGTGTGTGGCAGGCCCCAACATCGCCGCCATCGTCG
  GGGGCACCGTGGCAGGCATCGTGCTGATCGGCATTCTCCTGCTGGTCATCTGGAAGGCTC
  TGATCCACCTGAGCGACCTCCGGGAGTACAGGCGCTTTGAGAAGGAGAAGCTCAAGTCCC
  AGTGGAACAATGATAATCCCCTTTTCAAGAGCGCCACCACGACGGTCATGAACCCCAAGT
  TTGCTGAGAGTTAGGAGCACTTGGTGAAGACAAGGCCGTCAGGACCCACCATGTCTGCCC
  CATCACGCGGCCGAGACATGGCTTGCCACAGCTCTTGAGGATGTCACCAATTAACCAGAA
  ATCCAGTTATTTTCCACCCTCAAAATGACAGCCATGGCCGGCCGGGTGCTTCTGGGGGCT
  CGTCGGGGGGACAGCTCCACTCTGACTGGCACAGTCTTTGCATGGAGACTTGAGGAGGGA
  GGGCTTGAGGTTGGTGAGGTTAGGTGCGTGTTTCCTGTGCAAGTCAGGACATCAGTCTGA
  TTAAAGGTGGTGCCAATTTATTTACATTTAAACTTGTCAGGGTATAAAATGACATCCCAT
  TAATTATATTGTTAATCAATCACGTGTATAGAAAAAAAATAAAACTTCAATACAGGCTGT
  CCATGGAAAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_000211
• Insert Size: 2800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000211.2, NP_000202.2
• RefSeq Size: 2887 bp
• RefSeq ORF: 2310 bp
• Locus ID: 3689
• Cytogenetics: 21q22.3
• Domains: INB
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Leukocyte transendothelial migration, Natural killer cell mediated cytotoxicity, Regulation of actin cytoskeleton, Viral myocarditis
• Gene Summary: 'This gene encodes an integrin beta chain, which combines with multiple different alpha chains to form different integrin heterodimers. Integrins are integral cell-surface proteins that participate in cell adhesion as well as cell-surface mediated signalling. The encoded protein plays an important role in immune response and defects in this gene cause leukocyte adhesion deficiency. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Variants 1 and 2 encode the same isoform (1).