CRMP1 (NM_001313) Human Untagged Clone

CAT#: SC320180

CRMP1 (untagged)-Human collapsin response mediator protein 1 (CRMP1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CRMP1
• Synonyms: CRMP-1; DPYSL1; DRP-1; DRP1; ULIP-3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001313 edited
  ATGTCGTACCAGGGCAAGAAGAGCATCCCGCACATCACGAGTGACCGACTCCTCATCAAA
  GGTGGACGGATCATCAACGATGACCAATCCCTTTATGCTGACGTCTACCTGGAGGATGGA
  CTTATCAAACAAATAGGAGAGAACTTAATCGTTCCTGGTGGAGTGAAGACCATTGAAGCC
  AACGGGCGGATGGTTATTCCCGGAGGTATTGATGTCAACACGTACCTGCAGAAGCCCTCC
  CAGGGGATGACTGCGGCTGATGACTTCTTCCAAGGGACCAGGGCGGCACTGGTGGGCGGG
  ACCACGATGATCATTGACCATGTTGTTCCTGAACCTGGGTCCAGCCTACTGACCTCTTTC
  GAGAAGTGGCACGAAGCAGCTGACACCAAATCCTGCTGTGATTACTCCCTCCACGTGGAC
  ATCACAAGCTGGTACGATGGCGTTCGGGAGGAGCTGGAGGTGCTGGTGCAGGACAAAGGC
  GTCAATTCCTTCCAAGTCTACATGGCCTATAAGGATGTCTACCAAATGTCCGACAGCCAG
  CTCTATGAAGCCTTTACCTTCCTTAAGGGCCTGGGAGCTGTGATCTTGGTCCATGCAGAA
  AATGGAGATTTGATAGCTCAGGAACAAAAGCGGATCCTGGAGATGGGCATCACGGGTCCC
  GAGGGCCATGCCCTGAGCAGACCTGAAGAGCTGGAGGCCGAGGCGGTGTTCCGGGCCATC
  ACCATTGCGGGCCGGATCAACTGCCCTGTGTACATCACCAAGGTCATGAGCAAGAGTGCA
  GCCGACATCATCGCTCTGGCCAGGAAGAAAGGGCCCCTAGTTTTTGGAGAGCCCATTGCC
  GCCAGCCTGGGGACCGATGGCACCCATTACTGGAGCAAGAACTGGGCCAAGGCTGCGGCG
  TTCGTGACTTCCCCTCCCCTGAGCCCGGACCCTACCACGCCCGACTACTTGACCTCCCTA
  CTGGCCTGTGGGGACTTGCAGGTCACAGGCAGCGGCCACTGTCCCTACAGCACTGCCCAG
  AAGGCGGTGGGCAAGGACAACTTTACCCTGATCCCCGAGGGTGTCAACGGGATAGAGGAG
  CGGATGACCGTCGTCTGGGACAAGGCGGTGGCTACTGGCAAAATGGATGAGAACCAGTTT
  GTCGCTGTCACCAGCACCAATGCAGCCAAGATCTTTAACCTGTACCCAAGGAAAGGGCGG
  ATTGCCGTGGGCTCGGATGCCGACGTGGTCATCTGGGACCCCGACAAGTTGAAGACCATA
  ACAGCCAAAAGTCACAAGTCGGCGGTGGAGTACAACATCTTCGAGGGTATGGAGTGCCAC
  GGCTCCCCACTAGTGGTCATCAGCCAGGGCAAGATCGTCTTTGAAGACGGAAACATCAAC
  GTCAACAAGGGCATGGGCCGCTTCATTCCGCGGAAGGCGTTCCCGGAGCACCTGTACCAG
  CGCGTCAAAATCAGGAATAAGGTTTTTGGATTGCAAGGGGTTTCCAGGGGCATGTATGAC
  GGTCCTGTGTACGAGGTACCAGCTACACCCAAATATGCAACTCCCGCTCCTTCAGCCAAA
  TCTTCGCCTTCTAAACACCAGCCCCCACCCATCAGAAACCTCCACCAGTCCAACTTCAGC
  TTATCAGGTGCCCAGATAGATGACAACAATCCCAGGCGCACCGGCCACCGCATCGTGGCG
  CCCCCTGGTGGCCGCTCCAACATCACCAGCCTCGGTTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001313 unedited
  GATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTCGTGCCGA
  ATTCGGCACGAGGCATCCACGGGCGCCGAGCCTCCGTCCGTGTCTCTATCCCTCCCGGGC
  CTTTGTCAGCGCGCCCGCTGGGAGCGGGGCCGAGAGCGCCGGTTCCAGTCAGACAGCCCC
  GCAGGTCAGCGGCCGGGCCGAGGGCGCCAGAGGGGGCCATGTCGTACCAGGGCAAGAAGA
  GCATCCCGCACATCACGAGTGACCGACTCCTCATCAAAGGTGGACGGATCATCAACGATG
  ACCAATCCCTTTATGCTGACGTCTACCTGGAGGATGGACTTATCAAACAAATAGGAGAGA
  ACTTAATCGTTCCTGGTGGAGTGAAGACCATTGAAGCCAACGGGCGGATGGTTATTCCCG
  GAGGTATTGATGTCAACACGTACCTGCAGAAGCCCTCCCAGGGGATGACTGCGGCTGATG
  ACTTCTTCCAAGGGACCAGGGCGGCACTGGTGGGCGGGACCACGATGATCATTGACCATG
  TTGTTCCTGAACCTGNGTCCAGCCTACTGACCTCTTTCGAGAAGTGGCACGAAGCAGCTG
  ACACCAAATCCTGCTGTGATTACTCCCTCCACGTGGACATCACAAGCTGGTACGATGGCG
  TTCGGGAGGAGCTGGAGGTGCTGGTGCANGACAAAGGCGTCAATTCCTTCCAAGTCTACA
  TGGCCTATAAGGATGTCTACCAAATGTCCGACAGCCAGCTCTATGAAGCCCTTACCTTTC
  TTANGGGCCTGGAGCTGTGATCTTGGTCCATGCAGAAAATGGAGATTTGATAGCTCAGGA
  ACAAAGCGGATCTGGAGATGGCATCACGGTCCCGAGGGCATGCCCTGACAAACCTGAAAC
  CTGGAGCCCAGCGGTGGTTCCGGCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001313 unedited
  TGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGGCAGTTCATTTTCT
  TTATTTTTTCACAAGCTTTGAATTCAGAAATAAGAGCCACAACAAGTTGGTTGTTAAGTA
  AAAATGGGAGTGATTACAAAGGAAAACTTTGTACAAAACTTTAAAAATCTGACTGCCCCG
  TCAACTGCCTCAGAGGGACCAGGACTCAGGCCACGCAGGGGCAGCCATCAGCCCCCCATC
  GTCAGCCCCGAATGGGGATGGAGAGACCTCTTTCTGAGTGTGAACCTGGCTCGGCCTGAA
  GCCTAAAGCTGGCTTGAAGAACACACACAGACCAGAAGACAGCACGGTGAGTTACAAGTG
  CCACGGAGACCAGGGTTTCTGACACAGGATGTGGAGGATGCTTCTCTGCAACCTAGTGCA
  AGGCTTCCCCTGCCATTCCGAGAATTCACGGATCTCACAGGTGGAGGTGGGGCGAGCTTC
  TTTCTAGTGTGGACCAGAGGTGGGGGCAATGAGTAAACATCTTCACTGTAAGGGTTTTGG
  GACTGCCCCTCAGGGCTTGAGGTCTCGGCATCGTGTCTCAGTCAGTCCTTGGCGATGTCC
  CCTCAGACGCACTCACAGACTTGCATACGTAATTTAGTAACATGCATCAACTATCCTAGA
  ACATCTACAGGGTGCAAAGGAACCACCACTCAGAGAATCATGGAGCCAGTGGAGTTAGAA
  GTGGAAGGGACAGAACCAGACAATGCTAAAACCTGTGGTTCACTANGAANGGGGAATGAA
  AACACCATGCTCCGAGGTGGATTCAGCATGAACACAACTGTGGGGCAAGGGATTTCCAAG
  CAAACACCCACACTAGTTACACTTCTTTCTAAACAGAAAGGAAAGAGCATCCTTGACTTN
  CCCCTCCTNCATAANACCAACTAAACGTGGGGTTC
  
• Restriction Sites: Please inquire
• ACCN: NM_001313
• Insert Size: 3100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001313.3, NP_001304.1
• RefSeq Size: 3046 bp
• RefSeq ORF: 1719 bp
• Locus ID: 1400
• Cytogenetics: 4p16.2
• Domains: Amidohydro_1
• Gene Summary: 'This gene encodes a member of a family of cytosolic phosphoproteins expressed exclusively in the nervous system. The encoded protein is thought to be a part of the semaphorin signal transduction pathway implicated in semaphorin-induced growth cone collapse during neural development. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) contains an alternate 5' exon, compared to variant 1. It has a distinct 5' UTR and initiates translation at an alternate downstream AUG resulting in a protein (isoform 2) with a shorter N-terminus, compared to isoform 1.