CPSF30 (CPSF4) (NM_001081559) Human Untagged Clone

CAT#: SC320181

CPSF4 (untagged)-Human cleavage and polyadenylation specific factor 4, 30kDa (CPSF4), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CPSF4
• Synonyms: CPSF30; NAR; NEB-1; NEB1
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001081559.1 GCGAGAAGGCTGCAGGAGACCGAGGGGGAGCCGGGCCGGTGGGGCCGCCGCCGCCGCCAT
  GCAGGAAATCATCGCCAGCGTGGACCACATCAAGTTTGACTTGGAGATCGCGGTGGAGCA
  GCAGCTGGGGGCGCAGCCGCTGCCCTTCCCCGGCATGGACAAGTCGGGCGCTGCTGTCTG
  TGAATTCTTTTTGAAAGCTGCCTGCGGCAAAGGGGGCATGTGTCCGTTTCGCCACATCAG
  TGGTGAGAAGACAGTTGTGTGCAAACACTGGCTGCGTGGCCTATGCAAGAAAGGGGACCA
  GTGTGAGTTCCTGCATGAGTATGACATGACCAAGATGCCCGAGTGCTACTTCTACTCCAA
  GTTCGGGGAGTGCAGCAACAAGGAATGTCCCTTCCTGCACATCGACCCCGAGTCCAAGAT
  CAAGGACTGTCCTTGGTATGACCGTGGCTTCTGCAAGCACGGTCCCCTCTGCAGGCACCG
  GCACACACGGAGAGTCATCTGTGTGAATTACCTCGTGGGATTCTGCCCGGAGGGGCCCTC
  GTGTAAATTCATGCACCCTCGATTTGAACTGCCCATGGGAACCACCGAGCAGCCCCCACT
  GCCGCAGCAGACACAGCCTCCAGCAAAGCAGAGAACCCCGCAGGTCATCGGGGTCATGCA
  GAGTCAAAACAGCAGCGCGGGCAACCGGGGACCCCGGCCACTGGAGCAGGTCACCTGTTA
  CAAGTGTGGCGAGAAAGGACACTACGCCAACAGATGCACCAAAGGGCACTTGGCCTTTCT
  CAGTGGACAGTGACAGCAGCTGGAGCCAGCTCCGAGCAGCCCGGGGGCCCCGCTGTTGGG
  AGTGTGCATTTAACTGTTTCATGCGCTTGTTGGCGCGACTGTGGCTCGAGCTGGCCCGCA
  GACACGTGGGTTTCATCACTCTGAGGGGCCACGTCTGTTAGTTTCCTATCATTTTGCCTT
  AGTATTTTTTGAAAAAGGGACATGTGTCCTGTGGGTCCCTGCAGTCGACATCATGTTTGG
  CTGGGCATCGATGCCTCCTTTCTGGGACTCCCGGCACAACTCCCCTCATCCAGGGAGGGA
  GGCAGCTGCTGGGGAGGGGCTTGGCTAGGTAGTTCTGTGTGGCGGTGGTCATTCCCCTCA
  TTAAACACCAGTTCTTGGTGACGCCAGGGGCTGGTAGGTCATTCAAAGCTGTGGCCAGCT
  CACGCCTGCTTCCTCCCTCCCTGCCCTGCTGAATCCTAAAGCTGTGCCTATATCTGTGAT
  TTGAATGAGGGAGCCCTTTGGGGCAAATTCAGGTGCCCCCATTGCCTCAGGCTGGCCCTG
  GTCCCAGGTGGCAGCGGTTGAGGAGGGGTACAGGGCTCTCAAGCCTGAGGTTTTCTTCTC
  TGGGCTTAATTTTCTCTTGGGGTACGTGCCTGACAGTGTTTAAGGTGTCCGTTGAACTGG
  AGTTGCAGACTTTTAAATAGATGACCCCTTCAGATCATCTGTGCCTACCTCCTGCCCATC
  AGGCGTCTACACTGTCACTCAGACACCTGTGGCATGTGGAGGAGACTGCCCTGTCCTGAG
  CCTGGAAAATGTGAAACTGTCTCCTGCAACCTGCTGGGCATGTGGGCCTGGCTGTGTTCA
  ATTGCAAGAACAATTTTTATGAAATGGATTAAAGCTTGTTTTTTAAAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACCTCGGG
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001081559.1 unedited
  GGGACATTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACC
  GAGGAGATCTGCCGCCGCGATCGCGGCGCGCCAGATCTCAAGCTTAACTAGTTAGCGGAC
  CGACGCGTTAAGCGGCCGCGAATTCAGATCCACAAGTTTGTACAAAAAAGCAGGCTTGTA
  AAACGACGGCCAGTAACTATAACGGTCCTAAGGTAGCGAGGCCTGGGTGGCGAATTCGGC
  ACGAGGGCGAGAAGGCTGCAGGAGACCGAGGGGGAGCCGGGCCGGTGGGGCCGCCGCCGC
  CGCCATGCAGGAAATCATCGCCAGCGTGGACCACATCAAGTTTGACTTGGAGATCGCGGT
  GGAGCAGCAGCTGGGGGCGCAGCCGCTGCCCTTCCCCGGCATGGACAAGTCGGGCGCTGC
  TGTCTGTGAATTCTTTTTGAAAGCTGCCTGCGGCAAAGGGGGCATGTGTCCGTTTCGCCA
  CATCAGTGGTGAGAAGACAGTTGTGTGCAAACACTGGCTGCGTGGCCTATGCAAGAAGGG
  GACCAGTGTGAGTTCCTGCATGAGTATGACATGACCAAGATGCCCGAGTGCTACTTCTAC
  TCCAAGTTCGGGGAGTGCAGCAACAAGGAATGTCCCTTCCTGCACATCGACCCCGAGTCC
  AAGATCAAGGACTGTCCTTGGTATGACCGTGGCTTCTGCAAGCACGGTCCCCTCTGCAGG
  CACCGGCACACACGGAGAGTCATCTGTGTGAATTACCTCGTGGGATTCTGCCCGGAGGGC
  CCTCGTGTAAATTCATGCACCCCTCGATTTGAACTGCCCATGGGAACCACCGAGCAGCCC
  CCCACTGCCGCAGCAGACAAAGCCTCCAGCAAGCAGAGACCCCCGCAGTCATCGGGGTCA
  TGCAGAGTCAAAAACAGCAGCGCGCGGGCACCGGGGACCCCGGGCCACTGGGAGCAGGTC
  CACCCTGG
• Restriction Sites: Please inquire
• ACCN: NM_001081559
• ORF Size: 735 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_001081559.1, NP_001075028.1
• RefSeq Size: 1754
• RefSeq ORF: 735
• Locus ID: 10898
• Gene Summary: Inhibition of the nuclear export of poly(A)-containing mRNAs caused by the influenza A virus NS1 protein requires its effector domain. The NS1 effector domain functionally interacts with the cellular 30 kDa subunit of cleavage and polyadenylation specific factor 4, an essential component of the 3' end processing machinery of cellular pre-mRNAs. In influenza virus-infected cells, the NS1 protein is physically associated with cleavage and polyadenylation specific factor 4, 30kD subunit. Binding of the NS1 protein to the 30 kDa protein in vitro prevents CPSF binding to the RNA substrate and inhibits 3' end cleavage and polyadenylation of host pre-mRNAs. Thus the NS1 protein selectively inhibits the nuclear export of cellular, and not viral, mRNAs. Multiple alternatively spliced transcript variants that encode different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.