PLGF (PGF) (NM_002632) Human Untagged Clone

CAT#: SC320206

PGF (untagged)-Human placental growth factor (PGF), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PGF
• Synonyms: D12S1900; PGFL; PIGF; PLGF; PlGF-2; SHGC-10760
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002632.4 CTGCTGTCTGCGGAGGAAACTGCATCGACGGACGGCCGCCCAGCTACGGGAGGACCTGGA
  GTGGCACTGGGCGCCCGACGGACCATCCCCGGGACCCGCCTGCCCCTCGGCGCCCCGCCC
  CGCCGGGCCGCTCCCCGTCGGGTTCCCCAGCCACAGCCTTACCTACGGGCTCCTGACTCC
  GCAAGGCTTCCAGAAGATGCTCGAACCACCGGCCGGGGCCTCGGGGCAGCAGTGAGGGAG
  GCGTCCAGCCCCCCACTCAGCTCTTCTCCTCCTGTGCCAGGGGCTCCCCGGGGGATGAGC
  ATGGTGGTTTTCCCTCGGAGCCCCCTGGCTCGGGACGTCTGAGAAGATGCCGGTCATGAG
  GCTGTTCCCTTGCTTCCTGCAGCTCCTGGCCGGGCTGGCGCTGCCTGCTGTGCCCCCCCA
  GCAGTGGGCCTTGTCTGCTGGGAACGGCTCGTCAGAGGTGGAAGTGGTACCCTTCCAGGA
  AGTGTGGGGCCGCAGCTACTGCCGGGCGCTGGAGAGGCTGGTGGACGTCGTGTCCGAGTA
  CCCCAGCGAGGTGGAGCACATGTTCAGCCCATCCTGTGTCTCCCTGCTGCGCTGCACCGG
  CTGCTGCGGCGATGAGAATCTGCACTGTGTGCCGGTGGAGACGGCCAATGTCACCATGCA
  GCTCCTAAAGATCCGTTCTGGGGACCGGCCCTCCTACGTGGAGCTGACGTTCTCTCAGCA
  CGTTCGCTGCGAATGCCGGCCTCTGCGGGAGAAGATGAAGCCGGAAAGGAGGAGACCCAA
  GGGCAGGGGGAAGAGGAGGAGAGAGAAGCAGAGACCCACAGACTGCCACCTGTGCGGCGA
  TGCTGTTCCCCGGAGGTAACCCACCCCTTGGAGGAGAGAGACCCCGCACCCGGCTCGTGT
  ATTTATTACCGTCACACTCTTCAGTGACTCCTGCTGGTACCTGCCCTCTATTTATTAGCC
  AACTGTTTCCCTGCTGAATGCCTCGCTCCCTTCAAGACGAGGGGCAGGGAAGGACAGGAC
  CCTCAGGAATTCAGTGCCTTCAACAACGTGAGAGAAAGAGAGAAGCCAGCCACAGACCCC
  TGGGAGCTTCCGCTTTGAAAGAAGCAAGACACGTGGCCTCGTGAGGGGCAAGCTAGGCCC
  CAGAGGCCCTGGAGGTCTCCAGGGGCCTGCAGAAGGAAAGAAGGGGGCCCTGCTACCTGT
  TCTTGGGCCTCAGGCTCTGCACAGTCAAGCAGCCCTTGCTTTCGGAGCTCCTGTCCAAAA
  GTAGGGATGCGGATCCTGCTGGGGCCGCCACGGCCTGGCTGGTGGGAAGGCCGGCAGCGG
  GCGGAGGGGATCCAGCCACTTCCCCCTCTTCTTCTGAAGATCAGAACATTCAGCTCTGGA
  GAACAGTGGTTGCCTGGGGGCTTTTGCCACTCCTTGTCCCCCGTGATCTCCCCTCACACT
  TTGCCATTTGCTTGTACTGGGACATTGTTCTTTCCGGCCAAGGTGCCACCACCCTGCCCC
  CCCTAAGAGACACATACAGAGTGGGCCCCGGGCTGGAGAAAGAGCTGCCTGGATGAGAAA
  CAGCTCAGCCAGTGGGGATGAGGTCACCAGGGGAGGAGCCTGTGCGTCCCAGCTGAAGGC
  AGTGGCAGGGGAGCAGGTTCCCCAAGGGCCCTGGCACCCCCACAAGCTGTCCCTGCAGGG
  CCATCTGACTGCCAAGCCAGATTCTCTTGAATAAAGTATTCTAGTGTGGAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_002632
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002632.4, NP_002623.2
• RefSeq Size: 1758 bp
• RefSeq ORF: 513 bp
• Locus ID: 5228
• Cytogenetics: 14q24.3
• Domains: PDGF
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Bladder cancer, Focal adhesion, mTOR signaling pathway, Pancreatic cancer, Pathways in cancer, Renal cell carcinoma
• Gene Summary: 'This gene encodes a growth factor found in placenta which is homologous to vascular endothelial growth factor. Alternatively spliced transcripts encoding different isoforms have been found for this gene.[provided by RefSeq, Jun 2011]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).