PSMB8 (NM_004159) Human Untagged Clone

CAT#: SC320228

PSMB8 (untagged)-Human proteasome (prosome, macropain) subunit, beta type, 8 (large multifunctional peptidase 7) (PSMB8), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PSMB8
• Synonyms: ALDD; D6S216; D6S216E; JMP; LMP7; NKJO; PRAAS1; PSMB5i; RING10
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004159.4 AGCAACGCTAGGAAGGGCGGGCAGAAAGGGCACGCTCTTGTGGGTGACTACAGGTTAGGA
  GACCGTTGAACCTGGAGGGGCCCTAGGATGGACCCCGTGGAAAGATTCAGAGACTGCGCC
  CTCTCCCTGGCGCCGCCTTCCCCTACACGCGGCGGGTATATTCTGTTGCAGTTGGCCCAG
  GACCTGTTTCCAAGACTCTGCCCCCTCGCACTTCCGTCCCTCCTGGTTTTGTAAAGTGAT
  GCTCATAGGAACCCCCACCCCGCGTGACACTACTCCCAGCTCCTGGCTGACTTCTAGTCT
  TCTGGTTGAAGCTGCGCCTTTAGATGACACGACCCTACCCACCCCTGTTTCCAGCGGATG
  CCCGGGCCTGGAGCCCACAGAATTCTTCCAGTCCCTGGGTGGGGACGGAGAAAGGAACGT
  TCAGATTGAGATGGCCCATGGCACCACCACGCTCGCCTTCAAGTTCCAGCATGGAGTGAT
  TGCAGCAGTGGATTCTCGGGCCTCAGCTGGGTCCTACATTAGTGCCTTACGGGTGAACAA
  GGTGATTGAGATTAACCCTTACCTGCTTGGCACCATGTCTGGCTGTGCAGCAGACTGTCA
  GTACTGGGAGCGCCTGCTGGCCAAGGAATGCAGGCTGTACTATCTGCGAAATGGAGAACG
  TATTTCAGTGTCGGCAGCCTCCAAGCTGCTGTCCAACATGATGTGCCAGTACCGGGGCAT
  GGGCCTCTCTATGGGCAGTATGATCTGTGGCTGGGATAAGAAGGGTCCTGGACTCTACTA
  CGTGGATGAACATGGGACTCGGCTCTCAGGAAATATGTTCTCCACGGGTAGTGGGAACAC
  TTATGCCTACGGGGTCATGGACAGTGGCTATCGGCCTAATCTTAGCCCTGAAGAGGCCTA
  TGACCTTGGCCGCAGGGCTATTGCTTATGCCACTCACAGAGACAGCTATTCTGGAGGCGT
  TGTCAATATGTACCACATGAAGGAAGATGGTTGGGTGAAAGTAGAAAGTACAGATGTCAG
  TGACCTGCTGCACCAGTACCGGGAAGCCAATCAATAATGGTGGTGGTGGCAGCTGGGCAG
  GTCTCCTCTGGGAGGTCTTGGCCGACTCAGGGACCTAAGCCACGTTAAGTCCAAGGAGAA
  GAAGAGGCCTAGCCTGAGCCAAAGAGAGAGTACGGGCTCAGCAGCCAGAGGAGGCCGGTG
  AAGTGCATCTTCTGCGTGTTCTCTATTTGAACAAGCATTTCCCCCAGGGAAGTTTCTGGG
  TGCCCCACTAAGTAGAATAAAGAAAAACGGTTATAAATAAAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_004159
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004159.4, NP_004150.1
• RefSeq Size: 1602 bp
• RefSeq ORF: 819 bp
• Locus ID: 5696
• Cytogenetics: 6p21.32
• Protein Families: Druggable Genome, Protease
• Protein Pathways: Proteasome
• Gene Summary: 'The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. This gene is located in the class II region of the MHC (major histocompatibility complex). Expression of this gene is induced by gamma interferon and this gene product replaces catalytic subunit 3 (proteasome beta 5 subunit) in the immunoproteasome. Proteolytic processing is required to generate a mature subunit. Two alternative transcripts encoding two isoforms have been identified; both isoforms are processed to yield the same mature subunit. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (1) represents the longer transcript but encodes the shorter isoform (E1).