Grp75 (HSPA9) (NM_004134) Human Untagged Clone

CAT#: SC320242

HSPA9 (untagged)-Human heat shock 70kDa protein 9 (mortalin) (HSPA9), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HSPA9
• Synonyms: CRP40; CSA; EVPLS; GRP-75; GRP75; HEL-S-124m; HSPA9B; MOT; MOT2; MTHSP75; PBP74; SAAN; SIDBA4
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004134.4 GGAGCGCTTGTTTGCTGCCTCGTACTCCTCCATTTATCCGCCATGATAAGTGCCAGCCGA
  GCTGCAGCAGCCCGTCTCGTGGGCGCCGCAGCCTCCCGGGGCCCTACGGCCGCCCGCCAC
  CAGGATAGCTGGAATGGCCTTAGTCATGAGGCTTTTAGACTTGTTTCAAGGCGGGATTAT
  GCATCAGAAGCAATCAAGGGAGCAGTTGTTGGTATTGATTTGGGTACTACCAACTCCTGC
  GTGGCAGTTATGGAAGGTAAACGAGCAAAGGTGCTGGAGAATGCCGAAGGTGCCAGAACC
  ACCCCTTCAGTTGTGGCCTTTACAGCAGATGGTGAGCGACTTGTTGGAATGCCGGCCAAG
  CGACAGGCTGTCACCAACCCAAACAATACATTTTATGCTACCAAGCGTCTCATTGGCCGG
  CGATATGATGATCCTGAAGTACAGAAAGACATTAAAAATGTTCCCTTTAAAATTGTCCGT
  GCCTCCAATGGTGATGCCTGGGTTGAGGCTCATGGGAAATTGTATTCTCCGAGTCAGATT
  GGAGCATTTGTGTTGATGAAGATGAAAGAGACTGCAGAAAATTACTTGGGGCGCACAGCA
  AAAAATGCTGTGATCACAGTCCCAGCTTATTTCAATGACTCGCAGAGACAGGCCACTAAA
  GATGCTGGCCAGATATCTGGACTGAATGTGCTTCGGGTGATTAATGAGCCCACAGCTGCT
  GCTCTTGCCTATGGTCTAGACAAATCAGAAGACAAAGTCATTGCTGTATATGATTTAGGT
  GGTGGAACTTTTGATATTTCTATCCTGGAAATTCAGAAAGGAGTATTTGAGGTGAAATCC
  ACAAATGGGGATACCTTCTTAGGTGGGGAAGACTTTGACCAGGCCTTGCTACGGCACATT
  GTGAAGGAGTTCAAGAGAGAGACAGGGGTTGATTTGACTAAAGACAACATGGCACTTCAG
  AGGGTACGGGAAGCTGCTGAAAAGGCTAAGTGTGAACTCTCCTCATCTGTGCAGACTGAC
  ATCAATTTGCCCTATCTTACAATGGATTCTTCTGGACCCAAGCATTTGAATATGAAGTTG
  ACCCGTGCTCAATTTGAAGGGATTGTCACTGATCTAATCAGAAGGACTATCGCTCCATGC
  CAAAAAGCTATGCAAGATGCAGAAGTCAGCAAGAGTGACATAGGAGAAGTGATTCTTGTG
  GGTGGCATGACTAGGATGCCCAAGGTTCAGCAGACTGTACAGGATCTTTTTGGCAGAGCC
  CCAAGTAAAGCTGTCAATCCTGATGAGGCTGTGGCCATTGGAGCTGCCATTCAGGGAGGT
  GTGTTGGCCGGCGATGTCACGGATGTGCTGCTCCTTGATGTCACTCCCCTGTCTCTGGGT
  ATTGAAACTCTAGGAGGTGTCTTTACCAAACTTATTAATAGGAATACCACTATTCCAACC
  AAGAAGAGCCAGGTATTCTCTACTGCCGCTGATGGTCAAACGCAAGTGGAAATTAAAGTG
  TGTCAGGGTGAAAGAGAGATGGCTGGAGACAACAAACTCCTTGGACAGTTTACTTTGATT
  GGAATTCCACCAGCCCCTCGTGGAGTTCCTCAGATTGAAGTTACATTTGACATTGATGCC
  AATGGGATAGTACATGTTTCTGCTAAAGATAAAGGCACAGGACGTGAGCAGCAGATTGTA
  ATCCAGTCTTCTGGTGGATTAAGCAAAGATGATATTGAAAATATGGTTAAAAATGCAGAG
  AAATATGCTGAAGAAGACCGGCGAAAGAAGGAACGAGTTGAAGCAGTTAATATGGCTGAA
  GGAATCATTCACGACACAGAAACCAAGATGGAAGAATTCAAGGACCAATTACCTGCTGAT
  GAGTGCAACAAGCTGAAAGAAGAGATTTCCAAAATGAGGGAGCTCCTGGCTAGAAAAGAC
  AGCGAAACAGGAGAAAATATTAGACAGGCAGCATCCTCTCTTCAGCAGGCATCATTGAAG
  CTGTTCGAAATGGCATACAAAAAGATGGCATCTGAGCGAGAAGGCTCTGGAAGTTCTGGC
  ACTGGGGAACAAAAGGAAGATCAAAAGGAGGAAAAACAGTAATAATAGCAGAAATTTTGA
  AGCCAGAAGGACAACATATGAAGCTTAGGAGTGAAGAGACTTCCTGAGCAGAAATGGGCG
  AACTTCAGTCTTTTTACTGTGTTTTTGCAGTATTCTATATATAATTTCCTTAATTTGTAA
  ATTTAGTGACCATTAGCTAGTGATCATTTAATGGACAGTGATTCTAACAGTATAAAGTTC
  ACAATATTCTATGTCCCTAGCCTGTCATTTTTCAGCTGCATGTAAAAGGAGGTAGGATGA
  ATTGATCATTATAAAGATTTAACTATTTTATGCTGAAGTGACCATATTTTCAAGGGGTGA
  AACCATCTCGCACACAGCAATGAAGGTAGTCATCCATAGACTTGAAATGAGACCACATAT
  GGGGATGAGATCCTTCTAGTTAGCCTAGTACTGCTGTACTGGCCTGTATGTACATGGGGT
  CCTTCAACTGAGGCCTTGCAAGTCAAGCTGGCTGTGCCATGTTTGTAGATGGGGCAGAGG
  AATCTAGAACAATGGGAAACTTAGCTATTTATATTAGGTACAGCTATTAAAACAAGGTAG
  GAATGAGGCTAGACCTTTAACTTCCCTAAGGCATACTTTTCTAGCTACCTTCTGCCCTGT
  GTCTGGCACCTACATCCTTGATGATTGTTCTCTTACCCATTCTGGAATTTTTTTTTTTTT
  TAAATAAATACAGAAAGCATCTTGAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_004134
• Insert Size: 2800 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004134.4, NP_004125.3
• RefSeq Size: 2883 bp
• RefSeq ORF: 2040 bp
• Locus ID: 3313
• Cytogenetics: 5q31.2
• Domains: HSP70
• Protein Families: Stem cell - Pluripotency
• Protein Pathways: RNA degradation
• Gene Summary: 'This gene encodes a member of the heat shock protein 70 gene family. The encoded protein is primarily localized to the mitochondria but is also found in the endoplasmic reticulum, plasma membrane and cytoplasmic vesicles. This protein is a heat-shock cognate protein. This protein plays a role in cell proliferation, stress response and maintenance of the mitochondria. A pseudogene of this gene is found on chromosome 2.[provided by RefSeq, May 2010]'